Detailed information    

insolico Bioinformatically predicted

Overview

Name   uvrC   Type   Machinery gene
Locus tag   MPF99_RS00170 Genome accession   NZ_CP094130
Coordinates   29572..31356 (+) Length   594 a.a.
NCBI ID   WP_245058828.1    Uniprot ID   -
Organism   Helicobacter pylori strain Hpfe053     
Function   integration of short DNA fragment (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 24572..36356
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPF99_RS00135 (MPF99_00135) motA 24642..25415 (+) 774 WP_245058822.1 flagellar motor stator protein MotA -
  MPF99_RS00140 (MPF99_00140) motB 25418..26185 (+) 768 WP_245058824.1 flagellar motor protein MotB -
  MPF99_RS00145 (MPF99_00145) - 26189..26626 (+) 438 WP_001031792.1 hypothetical protein -
  MPF99_RS00150 (MPF99_00150) - 26726..28282 (+) 1557 WP_245058826.1 ABC transporter permease/substrate-binding protein -
  MPF99_RS00155 (MPF99_00155) - 28286..28927 (+) 642 WP_220886241.1 ATP-binding cassette domain-containing protein -
  MPF99_RS00160 (MPF99_00160) - 28939..29118 (-) 180 WP_096524500.1 hypothetical protein -
  MPF99_RS00165 (MPF99_00165) - 29110..29571 (+) 462 WP_000437191.1 hypothetical protein -
  MPF99_RS00170 (MPF99_00170) uvrC 29572..31356 (+) 1785 WP_245058828.1 excinuclease ABC subunit UvrC Machinery gene
  MPF99_RS00175 (MPF99_00175) - 31367..32632 (+) 1266 WP_245058830.1 homoserine dehydrogenase -
  MPF99_RS00180 (MPF99_00180) - 32633..32977 (+) 345 WP_001211650.1 YraN family protein -
  MPF99_RS00185 (MPF99_00185) trxA 33066..33386 (+) 321 WP_079367791.1 thioredoxin -
  MPF99_RS00190 (MPF99_00190) trxB 33392..34327 (+) 936 WP_245058832.1 thioredoxin-disulfide reductase -
  MPF99_RS00195 (MPF99_00195) - 34490..35311 (+) 822 WP_245058834.1 glycosyltransferase family 25 protein -
  MPF99_RS00200 (MPF99_00200) - 35515..35763 (-) 249 WP_000790557.1 RNA-binding protein -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 68858.16 Da        Isoelectric Point: 9.6559

>NTDB_id=667273 MPF99_RS00170 WP_245058828.1 29572..31356(+) (uvrC) [Helicobacter pylori strain Hpfe053]
MADLLSSLKNLSHSSGVYQYFDKNRQLLYIGKAKNLKKRIKSYFSIHNNEITPNYRASLRIQMMVKQIAFLETILVENEQ
DALILENSLIKQLKPKYNILLRDDKTYPYIYMDFSTDFPIPLITRKILKQPGVKYFGPFTSGAKDILDSLYELLPLVQKK
NCIKDKKACMFYQIERCKAPCENKITKEEYLKIAKECLEMIENKDKLIKELELKMERLSNNLRFEEALIYRDRIAKIQKI
APFTCMDLAKLYDLDIFAFYSGSNKAVLVKMFMRGGKIISSAFEKIHSLNGFDTDEAMKQAIINHYQSHLPLMPEQILLN
ACSNETLKELQEFIFHQYSKKIALSIPKKGDKLALIEIAMKNAQEIFSQEKTSNEDLILEEARSLFKLECMPYRVEIFDT
SHHANSQCVGGMVVYENHEFQKNSYRRYHLKGSNEYTQMSELLTRRALDFAKEPPPNLWVIDGGRAQLNIALEILKSSGS
FVEVIAISKEKRDSKAYRSKGGAKDIIHTANDTFKLLPSDKRLQWVQKLRDESHRYAINFHRSTKLKNMKQIALLKEKGI
GEASVKKLLDYFGSFEAIEKASEQEKNAVLKKRI

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=667273 MPF99_RS00170 WP_245058828.1 29572..31356(+) (uvrC) [Helicobacter pylori strain Hpfe053]
ATGGCTGATTTATTGTCCAGTTTAAAAAACCTTTCTCATAGCAGTGGCGTGTATCAATATTTTGATAAAAACCGCCAATT
ACTCTATATCGGTAAGGCGAAAAATTTAAAAAAGCGCATCAAAAGCTATTTTTCTATCCACAATAATGAAATCACGCCCA
ATTATCGCGCAAGCTTACGCATCCAAATGATGGTCAAACAGATCGCTTTTTTAGAAACCATTTTAGTGGAAAACGAACAA
GACGCTTTGATTTTAGAAAATTCTTTAATCAAACAGCTCAAGCCCAAATACAACATTCTTTTAAGAGATGATAAAACTTA
CCCTTATATTTATATGGATTTTTCCACTGATTTCCCTATCCCTTTAATCACACGAAAAATTTTAAAACAGCCTGGCGTTA
AATATTTTGGCCCTTTTACGAGCGGGGCTAAAGATATTTTAGACAGCTTGTATGAATTGCTCCCGTTAGTTCAAAAGAAA
AATTGCATCAAAGACAAAAAGGCATGCATGTTTTATCAAATAGAGCGTTGTAAAGCCCCATGCGAGAATAAAATCACCAA
AGAAGAGTATTTGAAAATCGCTAAAGAATGTTTAGAAATGATTGAAAATAAAGACAAACTCATCAAAGAGCTTGAATTAA
AAATGGAGCGCCTTTCTAATAATTTGCGTTTTGAAGAAGCCCTCATTTATAGGGATAGGATTGCAAAAATCCAAAAAATC
GCCCCTTTCACTTGCATGGATTTAGCCAAACTCTACGATTTGGATATTTTTGCTTTTTATAGTGGGAGCAATAAGGCGGT
GTTAGTGAAAATGTTTATGCGTGGGGGTAAAATCATTTCTTCAGCGTTTGAAAAAATCCACTCTCTTAATGGGTTTGACA
CTGATGAAGCGATGAAACAAGCCATTATCAATCATTACCAATCGCATTTGCCTTTGATGCCTGAACAGATTTTATTGAAC
GCTTGCTCTAATGAAACGCTTAAAGAATTGCAAGAATTTATCTTTCATCAATACTCTAAAAAAATCGCTCTTAGTATTCC
TAAAAAAGGCGATAAGCTCGCTTTAATAGAAATCGCTATGAAAAACGCTCAAGAGATTTTTAGCCAAGAAAAAACCTCTA
ATGAAGATCTGATTTTAGAAGAAGCGCGATCGCTCTTTAAATTAGAGTGCATGCCTTATAGGGTGGAAATCTTTGACACA
AGCCACCATGCCAACAGCCAATGCGTGGGGGGAATGGTCGTGTATGAAAATCATGAATTTCAAAAAAACTCCTATCGGCG
CTACCATTTAAAAGGCTCTAATGAATACACTCAAATGAGCGAATTGCTCACCAGAAGGGCTTTAGATTTTGCCAAAGAGC
CGCCGCCTAATTTGTGGGTGATCGATGGAGGGAGGGCGCAATTAAACATCGCTTTAGAAATTTTAAAAAGCAGCGGGAGT
TTTGTAGAAGTGATCGCTATTTCTAAAGAAAAAAGGGATTCTAAAGCTTATCGCTCCAAAGGGGGCGCTAAAGACATCAT
CCACACCGCTAACGATACTTTTAAATTGCTCCCTAGCGACAAGCGCTTGCAGTGGGTGCAAAAATTGCGCGATGAAAGCC
ACCGGTATGCGATAAACTTCCATAGATCCACTAAACTTAAAAACATGAAACAAATCGCTCTTTTAAAAGAAAAGGGTATA
GGAGAAGCCAGCGTGAAAAAATTGTTGGATTATTTTGGGAGTTTTGAAGCGATAGAAAAAGCGAGCGAACAGGAAAAAAA
CGCCGTTTTAAAAAAACGAATATAA

Domains

Predicted by InterproScan.

UvrB/uvrC motif (UVR)

(205-239)

GIY-YIG catalytic domain (GIY-YIG)

(15-98)

UvrC RNAse H endonuclease domain (UvrC_RNaseH_dom)

(385-538)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  uvrC Helicobacter pylori 26695

97.306

100

0.973

Multiple sequence alignment