Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MN200_RS13540 Genome accession   NZ_CP093968
Coordinates   2788015..2788815 (-) Length   266 a.a.
NCBI ID   WP_058870302.1    Uniprot ID   -
Organism   Acinetobacter sp. LUNF3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2783015..2793815
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MN200_RS13520 (MN200_13520) - 2784096..2784794 (-) 699 WP_234304490.1 tetratricopeptide repeat protein -
  MN200_RS13525 (MN200_13525) hisS 2784822..2786117 (-) 1296 WP_242759652.1 histidine--tRNA ligase -
  MN200_RS13530 (MN200_13530) ispG 2786114..2787229 (-) 1116 WP_004697677.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  MN200_RS13535 (MN200_13535) - 2787248..2788024 (-) 777 WP_058870301.1 helix-turn-helix domain-containing protein -
  MN200_RS13540 (MN200_13540) pilF 2788015..2788815 (-) 801 WP_058870302.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  MN200_RS13545 (MN200_13545) rlmN 2788832..2790067 (-) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  MN200_RS13550 (MN200_13550) ndk 2790190..2790621 (-) 432 WP_004983508.1 nucleoside-diphosphate kinase -
  MN200_RS13555 (MN200_13555) iscX 2790732..2790932 (-) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  MN200_RS13560 (MN200_13560) - 2791022..2791573 (-) 552 WP_058870303.1 phosphatase PAP2 family protein -
  MN200_RS13565 (MN200_13565) - 2791766..2793046 (-) 1281 WP_058870304.1 glycosyltransferase family 1 protein -
  MN200_RS13570 (MN200_13570) - 2793187..2793603 (-) 417 WP_004983505.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30044.07 Da        Isoelectric Point: 9.1790

>NTDB_id=666095 MN200_RS13540 WP_058870302.1 2788015..2788815(-) (pilF) [Acinetobacter sp. LUNF3]
MKKPVSKLCLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLRVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=666095 MN200_RS13540 WP_058870302.1 2788015..2788815(-) (pilF) [Acinetobacter sp. LUNF3]
TTGAAAAAACCCGTTTCTAAACTTTGCCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACTGT
GAATACGAAAGGTGATCCAAGTAAAGCAGTACAAGTTAGAACGCAGTTGGCGGCTGAATATATTAAGTCGAATGATTATG
ATGCTGCGAAACGTACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTGTTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCAGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCGAGTTG
CAGGTTCAACTTTAGGATATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCAGCTTTTAAGCAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCGGTTGGCGAGAAAAACTTAG
ATGCACGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAATCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
CTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_19)

(89-147)

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

69.03

100

0.695
  pilF Acinetobacter baylyi ADP1

64.662

100

0.647