Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   MPL63_RS12430 Genome accession   NZ_CP093947
Coordinates   2508740..2510548 (-) Length   602 a.a.
NCBI ID   WP_002288947.1    Uniprot ID   A0A132ZEU6
Organism   Enterococcus faecium strain NY11066     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2503740..2515548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPL63_RS12405 (MPL63_12405) - 2504780..2505679 (-) 900 WP_002294562.1 RluA family pseudouridine synthase -
  MPL63_RS12410 (MPL63_12410) - 2505681..2506478 (-) 798 WP_002289847.1 NAD kinase -
  MPL63_RS12415 (MPL63_12415) - 2506456..2507160 (-) 705 WP_002289848.1 GTP pyrophosphokinase family protein -
  MPL63_RS12420 (MPL63_12420) - 2507291..2507866 (+) 576 WP_002289849.1 CYTH domain-containing protein -
  MPL63_RS12425 (MPL63_12425) - 2507984..2508661 (+) 678 WP_002289850.1 ClpXP adapter SpxH family protein -
  MPL63_RS12430 (MPL63_12430) pepF 2508740..2510548 (-) 1809 WP_002288947.1 oligoendopeptidase F Regulator
  MPL63_RS12435 (MPL63_12435) - 2510724..2511722 (-) 999 WP_002288958.1 competence protein CoiA family protein -
  MPL63_RS12440 (MPL63_12440) - 2511948..2512601 (-) 654 WP_002288948.1 adaptor protein MecA -
  MPL63_RS12445 (MPL63_12445) - 2513035..2514330 (+) 1296 WP_002296623.1 ISL3 family transposase -
  MPL63_RS12450 (MPL63_12450) spxA 2514444..2514842 (-) 399 WP_002296384.1 transcriptional regulator SpxA -

Sequence

Protein


Download         Length: 602 a.a.        Molecular weight: 69702.99 Da        Isoelectric Point: 4.6502

>NTDB_id=665831 MPL63_RS12430 WP_002288947.1 2508740..2510548(-) (pepF) [Enterococcus faecium strain NY11066]
MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQSEKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAH
LKNDQDTGNTDYQALYARASSLFSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAAFKGLYSVYEQFRNTFASTLGTH
IKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVDVVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTY
EEAKEKALEALKPMGEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHEMGHSVHSY
FTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHYLDGFKGTVFRQTQFAEFEHFMHTEDEKGVP
LTSEYLSDSYGKLNAKYYGPAVEEDPEIKFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGN
SDYPVEVMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL

Nucleotide


Download         Length: 1809 bp        

>NTDB_id=665831 MPL63_RS12430 WP_002288947.1 2508740..2510548(-) (pepF) [Enterococcus faecium strain NY11066]
ATGGAAGTAAAGCAGTTGCCAAAACGAGAAGAATTGCCTGAAAATTTAACTTGGGACTTGACCAAGATCTTTTCAAGCGA
CCAAGAGTTTGATGAGAAATATTTGGAATTATCAGAAGAGTTAAAACAATCTGAAAAACACAAAGGAACACTTGATCAAG
GCGCTTCTCAATTTTTAAATGCGATTGAATTCGTATTGAGGGTTTATCGCCAAACTGAAGTCATTTATGTATATGCGCAC
CTTAAAAACGATCAAGACACTGGAAATACAGATTACCAAGCGCTTTATGCAAGAGCAAGCAGTCTGTTTTCGAAAGTTAG
TGAAGCCGTTTCCTGGTTTGAACCAGAAATATTGCAATTGTCAGATGACCAGATTTGGCAATATTTCAAAGAAGAACCAA
AATTGGAAGTCTATCGCCATTATATCCAGCAAATAGTAGATAATCGAGCCCATGTCTTATCTGCTGAGCAGGAATCTCTT
CTTGCTGGAGCAGGTGAAATCTTTGATGCTTCAAGTGATACATTTGCTGTTTTGAATAATGCAGATCTAGTTTTTCCAAC
GATTGAAGGAGAAAATGGTGAAATAGTCCAATTATCTCATGGCGTGTATGGTCAGTTGCTAGAAAGCACGGATCGAAGGG
TGCGCGAAGCAGCATTTAAGGGATTGTACAGTGTTTACGAACAATTTAGAAATACATTTGCTTCTACTTTAGGCACACAT
ATAAAAGGACATAATTTTAAAGCGAAAGTCCGTAATTACAGCTCTGCCAGAGAAGCGTCTTTGAGCAATAATCATATTCC
TGAAAGTGTATACGATACTTTGGTAGACGTGGTAAACAAGCATTTGCCTTTGTTACATCGATACATGGAATTACGGAAAC
GTTTATTAGAAGTGGAAAAACTGCACATGTATGATCTTTATACACCGGTCTTAGGGGAAGCTCCAATTACCTTTACGTAC
GAAGAAGCAAAAGAAAAAGCTTTAGAAGCACTGAAACCAATGGGTGAAGAATACATGGCCATCGTAGAAAAAGCATTCTC
TGAACGTTGGATCGATGTTGTCGAAAATAAAGGGAAACGAAGCGGTGCTTATTCTTCGGGAAGCTATGACACAAATCCAT
ATATTTTATTGAATTGGCATGATACGCTGGATCAGCTATTTACGCTTGTCCACGAAATGGGACATAGTGTTCATAGTTAT
TTCACTCGTTCGAACCAGCCTTATGTGTACGGCGACTACTCCATCTTTTTAGCAGAAATTGCTTCGACAACGAATGAAAA
TATCCTAACGGAGTATTTATTGGAAACAGAAAAAGACCCTCGTGTACGGGCTTATGTACTCAACCATTACTTGGATGGGT
TTAAAGGAACAGTTTTCCGCCAGACACAATTCGCTGAATTCGAGCATTTCATGCATACGGAAGATGAAAAAGGTGTGCCA
TTGACTAGTGAATACCTAAGTGATAGTTATGGTAAATTGAATGCAAAATATTATGGTCCAGCAGTCGAAGAAGACCCAGA
AATTAAATTCGAATGGTCACGGATACCGCATTTTTATTATAATTACTATGTTTTCCAATACTCGACTGGCTTTTCTGCTG
CTTCAGCACTGGCGAAGAAAATACTAAACCAGGAACCAGAGGCACTAGAGAACTACTTGGCTTACCTAAAAGCAGGTAAC
AGCGATTATCCTGTGGAAGTAATGAAAAAAGCAGGAGTTGATATGACACAGGCTGCATATATTGAAGATGCAATGTCGAT
GTTTGAACAACGTCTAAATGAATTAGAAGAATTGATCGATCGTTTATAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(116-185)

Peptidase family M3 (Peptidase_M3)

(206-586)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A132ZEU6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

55.649

98.505

0.548