Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MNY32_RS15435 Genome accession   NZ_CP093535
Coordinates   3302163..3303662 (+) Length   499 a.a.
NCBI ID   WP_064477487.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain SL1931     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3297163..3308662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNY32_RS15410 (MNY32_15410) - 3298356..3299339 (+) 984 WP_016724265.1 KpsF/GutQ family sugar-phosphate isomerase -
  MNY32_RS15415 (MNY32_15415) - 3299378..3299965 (+) 588 WP_011000373.1 KdsC family phosphatase -
  MNY32_RS15420 (MNY32_15420) lptC 3299967..3300599 (+) 633 WP_011000372.1 LPS export ABC transporter periplasmic protein LptC -
  MNY32_RS15425 (MNY32_15425) lptA 3300668..3301243 (+) 576 WP_011000371.1 lipopolysaccharide transport periplasmic protein LptA -
  MNY32_RS15430 (MNY32_15430) lptB 3301277..3302065 (+) 789 WP_016724264.1 LPS export ABC transporter ATP-binding protein -
  MNY32_RS15435 (MNY32_15435) rpoN/rpoN1 3302163..3303662 (+) 1500 WP_064477487.1 RNA polymerase factor sigma-54 Machinery gene
  MNY32_RS15440 (MNY32_15440) hpf 3303714..3304067 (+) 354 WP_011000368.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  MNY32_RS15445 (MNY32_15445) ptsN 3304416..3304871 (+) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  MNY32_RS15450 (MNY32_15450) hprK 3304942..3305916 (+) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  MNY32_RS15455 (MNY32_15455) - 3306107..3306442 (+) 336 WP_020425146.1 PsiF family protein -
  MNY32_RS15460 (MNY32_15460) rapZ 3306536..3307426 (+) 891 WP_058907464.1 RNase adapter RapZ -
  MNY32_RS15465 (MNY32_15465) - 3307504..3308154 (+) 651 WP_016724261.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55257.58 Da        Isoelectric Point: 4.8064

>NTDB_id=665068 MNY32_RS15435 WP_064477487.1 3302163..3303662(+) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain SL1931]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSSSATPLPTE
SPPPAANGADHADTAEASYENGSDFDSDYGTERSDWSLDDFARKPQSDEDDRAPMQLREAEPTQREFLMEQLAPLKLSPR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
TIVNQHLELLAARDYTRLKKALSVDDGELKAAHELIRSLAPFPGHTFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=665068 MNY32_RS15435 WP_064477487.1 3302163..3303662(+) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain SL1931]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCCCAGCATCTGGCGCTCACGCCACAACTGCAGCAGTCGATCCGGCTACTGCA
GTTGTCGACCATCGAGCTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTCGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGACGGCTCGGTCAACGCCCTGCAGAGCAGCAGCGCCACCCCGCTGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGCGCGGACCATGCCGACACGGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTACGGCACCGAGCGGTCCGACTGGAGCCTCGACGACTTCGCGCGCAAACCCCAGTCCGACGAAGACGACCGCGCGC
CGATGCAGCTGCGCGAGGCCGAACCCACGCAGCGCGAATTCCTGATGGAACAACTCGCGCCGCTAAAGCTGTCCCCGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAGCTCGACGAATTGCAGGCCGCGCTGCGGATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGTGCGCGCTCGGCGGCCGAATGCCTGACGCTGCAGCTGCAGCGCCTGGACTCCCCGGCCAAGCCGCTGGCGCTG
ACCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGGCTGAAGAAAGCGTTGTCCGTCGATGATGG
CGAGCTGAAGGCCGCGCATGAGCTGATCCGCTCGCTGGCGCCCTTTCCTGGCCACACCTTCGGGCGGGCCGAGGCCGACT
TCGTCGTGCCCGACGTGGTGGTGCGCAAGACCGCCGCCGGCTGGATGGCACAGCTGAACCCCGACGTGATGCCACGCCTG
CGCATCAACGACATGTATGCGCAGATCCTGCGCAGCAGCCGTGGCGAATCGGGTGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCAATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAATACATGGCAACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAGATCCCAGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

99.8

100

0.998