Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MNY32_RS10315 Genome accession   NZ_CP093535
Coordinates   2254920..2256290 (-) Length   456 a.a.
NCBI ID   WP_016722028.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain SL1931     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2249920..2261290
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNY32_RS10295 (MNY32_10295) - 2250763..2252694 (+) 1932 WP_043886002.1 ATP-binding cassette domain-containing protein -
  MNY32_RS10300 (MNY32_10300) - 2252726..2253832 (+) 1107 WP_021154814.1 CaiB/BaiF CoA transferase family protein -
  MNY32_RS10305 (MNY32_10305) - 2253815..2254336 (-) 522 WP_021154815.1 DUF523 domain-containing protein -
  MNY32_RS10310 (MNY32_10310) - 2254347..2254826 (-) 480 WP_011001322.1 disulfide bond formation protein B -
  MNY32_RS10315 (MNY32_10315) radA 2254920..2256290 (-) 1371 WP_016722028.1 DNA repair protein RadA Machinery gene
  MNY32_RS10320 (MNY32_10320) alr 2256370..2257497 (-) 1128 WP_020831897.1 alanine racemase -
  MNY32_RS10325 (MNY32_10325) lplT 2258128..2259444 (+) 1317 WP_011001319.1 lysophospholipid transporter LplT -
  MNY32_RS10330 (MNY32_10330) - 2259565..2260545 (-) 981 WP_165858477.1 uracil-DNA glycosylase -
  MNY32_RS10335 (MNY32_10335) rimI 2260616..2261149 (-) 534 WP_021154817.1 ribosomal protein S18-alanine N-acetyltransferase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 48397.86 Da        Isoelectric Point: 7.6668

>NTDB_id=665058 MNY32_RS10315 WP_016722028.1 2254920..2256290(-) (radA) [Ralstonia pseudosolanacearum strain SL1931]
MAKSKTVYTCTECGGTSPKWAGQCPHCQQWNTLVETVAQPVSGAGARFQSLAASAVVRKLADIDAVDVPRFSSGIDEFDR
VLGGGLVGGGVVLIGGDPGIGKSTLLLQALSNLAASRRVLYVSGEESGAQIALRARRLGVESPNLALLAEIQLERIQATI
EAEKPEVVVIDSIQTLYSEALTSAPGSVAQVRECAAQLTRIAKRLDVTTILVGHVTKEGALAGPRVLEHIVDTVLYFEGD
THSAYRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHEQTVPGSCVLVTQEGTRPLLVEIQALVDAAGVPNP
RRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAIHSSMRNKPLPRGLVVFGEIGLAGEI
RPTPRGQERLKEAAKLGFSIAVIPKSNAPKQPIDGLQVIAVERIEQAIDRVRGLEA

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=665058 MNY32_RS10315 WP_016722028.1 2254920..2256290(-) (radA) [Ralstonia pseudosolanacearum strain SL1931]
TTGGCCAAGAGCAAGACGGTCTATACGTGCACCGAGTGCGGCGGCACGTCGCCGAAATGGGCGGGGCAGTGCCCGCATTG
CCAGCAGTGGAACACGCTGGTGGAAACCGTGGCGCAGCCGGTGTCCGGCGCCGGCGCGCGCTTCCAGTCGCTGGCGGCGA
GCGCCGTGGTGCGCAAGCTGGCGGACATCGACGCCGTGGACGTGCCGCGCTTCTCCAGCGGCATCGACGAATTCGACCGC
GTGCTGGGCGGCGGCCTGGTCGGCGGCGGCGTGGTGCTGATCGGCGGCGATCCGGGCATCGGCAAGTCGACGCTGCTGCT
GCAGGCGCTGTCCAACCTGGCGGCCAGCCGGCGCGTGCTGTATGTGAGCGGCGAGGAGTCCGGCGCGCAGATCGCGCTGC
GGGCACGGCGCCTGGGCGTCGAAAGCCCGAACCTGGCGCTGCTGGCCGAGATCCAGCTCGAGCGCATCCAGGCCACCATC
GAGGCGGAGAAGCCCGAGGTGGTCGTCATCGACTCCATCCAGACGCTGTACTCGGAGGCGCTGACCTCGGCGCCGGGCTC
GGTGGCCCAGGTGCGCGAATGCGCGGCGCAGCTCACGCGCATCGCCAAGCGGCTGGACGTGACCACCATCCTGGTCGGCC
ACGTGACCAAGGAGGGCGCACTGGCGGGCCCGCGCGTGCTGGAGCACATCGTCGATACGGTGCTGTATTTCGAGGGCGAC
ACGCATTCGGCCTACCGGCTGGTGCGCGCGTTCAAGAACCGCTTCGGCGCGGTCAACGAGCTGGGCGTGTTCGCCATGAC
CGAGCGCGGCCTGCGTGGCGTGGCGAATCCGTCGGCGCTGTTCCTGTCGCAGCACGAGCAGACCGTGCCGGGTTCGTGCG
TGCTGGTGACGCAGGAGGGCACGCGTCCGCTGCTGGTGGAGATCCAGGCGCTGGTGGATGCGGCGGGCGTGCCGAACCCG
CGGCGGCTGGCGGTGGGCCTGGAGCAGAACCGGCTGGCGATGCTGCTGGCGGTGCTGCACCGGCACGCGGGCATTGCCTG
CTTTGACCAGGACGTGTTCCTCAATGCCGTGGGCGGGGTCAAGATCACCGAGCCGGCGGCGGACCTGGCGGTGCTGCTGG
CGATCCATTCGTCGATGCGCAACAAGCCGCTGCCGCGCGGGCTGGTGGTTTTCGGCGAGATCGGGCTGGCCGGCGAGATC
CGTCCGACGCCGCGCGGGCAGGAGCGCCTGAAGGAAGCGGCCAAGCTGGGCTTCTCGATTGCCGTGATCCCCAAGTCGAA
CGCGCCCAAGCAGCCGATCGACGGCCTGCAGGTGATCGCCGTCGAGCGCATCGAGCAGGCGATCGACCGGGTGCGCGGGC
TGGAGGCTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(362-448)

AAA domain (AAA_25)

(65-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.684

100

0.487
  radA Streptococcus pneumoniae Rx1

46.93

100

0.469
  radA Streptococcus pneumoniae D39

46.93

100

0.469
  radA Streptococcus pneumoniae R6

46.93

100

0.469
  radA Streptococcus pneumoniae TIGR4

46.93

100

0.469
  radA Streptococcus mitis SK321

47.124

99.123

0.467
  radA Streptococcus mitis NCTC 12261

46.903

99.123

0.465