Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MON12_RS03280 Genome accession   NZ_CP093424
Coordinates   644103..645479 (-) Length   458 a.a.
NCBI ID   WP_001085212.1    Uniprot ID   A0A2B3Y2M8
Organism   Bacillus pacificus strain MP6     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 639103..650479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MON12_RS03260 (MON12_03260) ispF 640467..640943 (-) 477 WP_000488396.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  MON12_RS03265 (MON12_03265) ispD 641059..641739 (-) 681 WP_000288294.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MON12_RS03270 (MON12_03270) - 641756..642865 (-) 1110 WP_000919684.1 PIN/TRAM domain-containing protein -
  MON12_RS03275 (MON12_03275) disA 643026..644099 (-) 1074 WP_074567820.1 DNA integrity scanning diadenylate cyclase DisA -
  MON12_RS03280 (MON12_03280) radA 644103..645479 (-) 1377 WP_001085212.1 DNA repair protein RadA Machinery gene
  MON12_RS03285 (MON12_03285) clpC 645576..648011 (-) 2436 WP_000971179.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  MON12_RS03290 (MON12_03290) - 648034..649098 (-) 1065 WP_000050822.1 protein arginine kinase -
  MON12_RS03295 (MON12_03295) - 649103..649651 (-) 549 WP_014893380.1 UvrB/UvrC motif-containing protein -
  MON12_RS03300 (MON12_03300) ctsR 649825..650286 (-) 462 WP_001244563.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49858.44 Da        Isoelectric Point: 7.1421

>NTDB_id=664599 MON12_RS03280 WP_001085212.1 644103..645479(-) (radA) [Bacillus pacificus strain MP6]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
THIEEVNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=664599 MON12_RS03280 WP_001085212.1 644103..645479(-) (radA) [Bacillus pacificus strain MP6]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGGTATCAGTCACCAAAATATATGGGGAAATGCCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCAGTTGTATCATCAAGGCGCCTTAATTATGCCAATGCAATTCAAA
CAGAAGTAACAAAACCAAGACGTCTAACAGAAGTGGAAACAAAGTCTGAGGCGCGTATTGAAACAAAATTTCAAGAATTT
AACCGTGTACTTGGTGGCGGGATTGTAGATGGATCCTTAGTACTGATTGGTGGAGACCCCGGGATTGGAAAATCAACGTT
ACTGTTACAAATATCATCGCAATTAGCAGATTCTTCATATGATGTCCTATATATATCGGGTGAAGAATCAGCAAAACAGA
TTAAACTGCGTGCGGATCGTTTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACAGATTTACAGCGAATTGCA
ACACATATTGAAGAGGTGAACCCAGCCTTTGTTGTTATTGATTCTATTCAAACAATACATCTACCTGAAGTAACATCAGC
GCCGGGAAGTGTGGCACAAGTTCGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACGAAAGGGATCCCTATTTTTA
TCGTTGGACATGTGACAAAAGAGGGAGCGATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGACCGTCATCATACATACCGCATTTTGCGAGCAGTAAAGAATCGTTTTGGTTCTACGAATGAAATGGGTATTTT
TGAAATGAAAGAGCTTGGCCTTGCAGAAGTATTAAACCCTTCTGAAATTTTTCTTGAGGAAAGACCAGTTGGGGTTGCAG
GATCAACAGTGGTTGCCTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCCACGGGAATTGATCATAACCGTGTATCGCTTATTATGGCAGTGCTAGAAAAAAGAACAGG
CTTATTATTGCAAAATCAGGATGCATATTTAAAAGTAGCAGGTGGTTTGAAGTTAGATGAACCAGCAATTGATTTAGCTG
TCGCTTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCAACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGCGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCAGATGGGATTGAGGTAGTTGGTGTATCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B3Y2M8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.946

100

0.751
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.576

98.908

0.629