Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MN187_RS08595 Genome accession   NZ_CP093384
Coordinates   1780992..1782362 (-) Length   456 a.a.
NCBI ID   WP_117973397.1    Uniprot ID   -
Organism   Vagococcus sp. CY52-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1775992..1787362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MN187_RS08575 (MN187_08575) - 1776229..1776648 (-) 420 WP_117973393.1 Mini-ribonuclease 3 -
  MN187_RS08580 (MN187_08580) cysS 1776645..1778063 (-) 1419 WP_117973394.1 cysteine--tRNA ligase -
  MN187_RS08585 (MN187_08585) gltX 1778310..1779785 (-) 1476 WP_117973395.1 glutamate--tRNA ligase -
  MN187_RS08590 (MN187_08590) - 1779886..1780971 (-) 1086 WP_117973396.1 PIN/TRAM domain-containing protein -
  MN187_RS08595 (MN187_08595) radA 1780992..1782362 (-) 1371 WP_117973397.1 DNA repair protein RadA Machinery gene
  MN187_RS08600 (MN187_08600) - 1782472..1782999 (-) 528 WP_117973398.1 dUTP diphosphatase -
  MN187_RS08605 (MN187_08605) - 1783088..1785070 (-) 1983 WP_241699521.1 KUP/HAK/KT family potassium transporter -
  MN187_RS08610 (MN187_08610) proB 1785316..1786119 (+) 804 WP_241699501.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50047.47 Da        Isoelectric Point: 7.3272

>NTDB_id=664230 MN187_RS08595 WP_117973397.1 1780992..1782362(-) (radA) [Vagococcus sp. CY52-2]
MAKKKRNEFVCQTCGYISPKYLGRCPNCGEWNTLVEEISSDTPTRHSRVSLTGEMSKPQKLKEVATQKERRIKTQLGELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLSLLKGNVLYVSGEESSQQIKMRAERLADGTNDFYVYPETDMGRIKQ
VIEQLTPDYVIIDSIQTMVHPETESAAGSVSQVRANTAELMQIAKTNNIAIFIVGHVTKEGSLAGPRMLEHMVDTVLYFE
GDRHHTFRILRAVKNRFGSTNEIGIFEMRQAGLVEVLNPSEAFLEERIDGATGSAIVCSMEGTRPILAEIQSLVTPSVFG
NAKRTATGLDYNRVSLIMAVLEKRAGLLLQNQDAYLKAVGGVKLDEPAIDLSIAVSIASSYKDKGTSPQDCFIGEIGLTG
EIRRVNRIEQRVGEAQKLGFRRVFIPINNLQGWTPPTGIEVIGVETLSETLFKVFS

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=664230 MN187_RS08595 WP_117973397.1 1780992..1782362(-) (radA) [Vagococcus sp. CY52-2]
GTGGCAAAAAAAAAGAGAAATGAGTTTGTTTGTCAAACGTGTGGTTATATCTCTCCAAAGTATCTGGGTCGTTGTCCTAA
TTGTGGTGAATGGAATACATTAGTGGAAGAAATTTCCTCAGATACCCCCACTAGGCATAGTCGAGTAAGTTTGACGGGTG
AAATGAGTAAACCGCAAAAGTTAAAAGAAGTCGCTACACAAAAAGAACGGCGTATAAAAACACAATTAGGTGAATTAAAT
AGAGTATTAGGCGGGGGGGTTGTTCCAGGCTCAATGGTCTTAATTGGTGGGGATCCTGGTATTGGAAAATCAACACTATT
ACTACAAGTATCTCAACAACTTAGTCTGTTAAAAGGAAATGTATTATACGTCTCTGGTGAAGAGAGTTCGCAACAGATTA
AAATGCGTGCAGAACGGTTGGCTGATGGAACAAATGATTTTTATGTTTATCCAGAAACGGATATGGGTCGCATTAAACAA
GTTATCGAGCAATTGACACCAGACTATGTGATTATTGATTCGATTCAGACAATGGTTCATCCGGAAACGGAAAGTGCTGC
TGGAAGTGTGAGTCAAGTTCGAGCGAATACGGCTGAGTTGATGCAAATTGCTAAAACAAACAATATTGCCATCTTCATTG
TAGGACACGTGACCAAAGAAGGCTCACTTGCAGGGCCACGGATGCTTGAACATATGGTAGATACAGTACTTTATTTTGAA
GGAGACAGACATCATACATTTAGAATATTGCGTGCTGTAAAAAATCGTTTTGGTTCAACCAATGAAATAGGTATTTTTGA
AATGAGACAAGCTGGTTTAGTGGAAGTGTTAAATCCATCTGAAGCTTTCTTGGAAGAGAGAATTGATGGGGCAACTGGAT
CAGCGATTGTCTGTTCAATGGAAGGAACGCGACCTATCTTAGCTGAAATTCAATCACTTGTAACGCCATCTGTTTTTGGA
AATGCGAAACGAACAGCAACAGGTCTTGATTATAACCGTGTATCACTGATTATGGCTGTGTTAGAAAAACGTGCTGGATT
ATTATTACAAAACCAAGATGCTTATTTAAAAGCAGTTGGGGGAGTGAAACTTGATGAACCAGCGATTGATTTATCCATCG
CAGTGAGTATTGCATCAAGTTACAAAGATAAAGGGACTTCTCCTCAAGATTGTTTTATTGGTGAAATTGGGTTAACTGGT
GAAATTAGACGAGTAAATCGTATCGAGCAACGGGTAGGAGAAGCACAGAAATTAGGATTTAGACGAGTCTTTATTCCTAT
TAATAATCTGCAAGGGTGGACACCGCCAACTGGAATTGAAGTAATTGGTGTTGAAACACTATCTGAAACATTATTTAAAG
TATTTTCCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(366-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

73.246

100

0.732
  radA Streptococcus mitis NCTC 12261

73.246

100

0.732
  radA Streptococcus pneumoniae Rx1

73.026

100

0.73
  radA Streptococcus pneumoniae D39

73.026

100

0.73
  radA Streptococcus pneumoniae R6

73.026

100

0.73
  radA Streptococcus pneumoniae TIGR4

73.026

100

0.73
  radA Bacillus subtilis subsp. subtilis str. 168

66.446

99.342

0.66