Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   AS4_RS19745 Genome accession   NZ_AP014630
Coordinates   4222597..4224822 (+) Length   741 a.a.
NCBI ID   WP_034617817.1    Uniprot ID   A0AAX3PLU7
Organism   Acinetobacter guillouiae strain NBRC 110550     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4217597..4229822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS4_RS19720 (AS4_39790) - 4219103..4219441 (-) 339 WP_004816760.1 tRNA-binding protein -
  AS4_RS19725 (AS4_39800) - 4219792..4220055 (+) 264 WP_034617824.1 DUF2798 domain-containing protein -
  AS4_RS19730 (AS4_39810) - 4220129..4220464 (-) 336 WP_004725552.1 hypothetical protein -
  AS4_RS19735 (AS4_39820) gspG 4220746..4221183 (-) 438 WP_004816754.1 type II secretion system major pseudopilin GspG -
  AS4_RS19740 (AS4_39830) gspF 4221223..4222428 (-) 1206 WP_096735249.1 type II secretion system inner membrane protein GspF -
  AS4_RS19745 (AS4_39840) priA 4222597..4224822 (+) 2226 WP_034617817.1 primosomal protein N' Machinery gene
  AS4_RS19750 (AS4_39850) - 4224926..4225426 (-) 501 WP_126606946.1 hypothetical protein -
  AS4_RS19755 (AS4_39860) - 4225613..4225954 (-) 342 WP_227548102.1 hypothetical protein -

Sequence

Protein


Download         Length: 741 a.a.        Molecular weight: 84288.66 Da        Isoelectric Point: 8.8802

>NTDB_id=66379 AS4_RS19745 WP_034617817.1 4222597..4224822(+) (priA) [Acinetobacter guillouiae strain NBRC 110550]
MSNTLATPQTIYRIQVAVPVYLNDCFDYQVTAEQYAQAEVGARVAVSFGRQNLIGVIVKKVPIDEPVDSHFKLKAITELL
DEQAILDSKVLSLLTWSSQYYQFPIGEVIQTALPTLLRQGKPYNLLARIWKLLDLHAEDKVKRSEKQQEAYKILKLHPKG
TQENILNLSGVETATLKVLEKKDIVRCDLEPQNVTPEPVQLAQMPLTPNDDQKKAIQAILKAQKKYQAFLLDGLTGSGKT
EVYLQVMHEVLKQGKQVLVLVPEIGLTPQTISRFQSRFHCNIALLHSGLNDTKRLQAWQHAQTGKASIILGTRSAIYTPL
PHLGLIILDEEHDLSFKQQEGFRYHARDVAMYRAHLENCPIILGSATPSIDSYYLARQEKLTRIELNQRAGAAIMPKMHL
IDLKVAKKQHGLSDVLIAEIKKRLEKKEQVLIFLNRRGYAPVLICESCGWQANCPNCDAHFTLHLKPYQHLHCHHCGTIN
RMPDACPQCNQQQLKPIGMGTAKVEETLNELFPHFPVLRVDRDSTSRVGSWQKIYDQIQKSEPTILLGTQMLAKGHHFPY
VTLVAILDIDAGLLSVDFRATERTAQLIIQVAGRAGRGEMKGEVYLQTLRPDHPLLNTLIHNDYRTFAKQTLKDRQAALL
PPFRYTALIRCDSKHQEENLKFLTEMTQLLRQTAEENLIDIWGPIPAPMERKAGRYQAHVVLLSQDRAKMHFYIRQWWQQ
LLHSKPSTMKVTIDVDPQELS

Nucleotide


Download         Length: 2226 bp        

>NTDB_id=66379 AS4_RS19745 WP_034617817.1 4222597..4224822(+) (priA) [Acinetobacter guillouiae strain NBRC 110550]
ATGTCGAATACCCTTGCTACGCCACAGACGATTTATCGTATTCAAGTTGCTGTTCCAGTCTATTTGAATGACTGCTTTGA
TTACCAAGTCACCGCTGAACAATATGCGCAAGCTGAAGTCGGTGCGCGTGTTGCAGTGTCTTTTGGCAGACAAAATCTGA
TTGGTGTTATTGTCAAGAAAGTACCGATTGATGAACCTGTGGATTCTCATTTTAAACTCAAAGCCATTACTGAACTGTTA
GATGAACAAGCCATTTTAGACAGTAAAGTTTTAAGTTTATTGACATGGTCATCACAATATTACCAATTTCCCATCGGTGA
AGTGATTCAAACGGCGTTACCTACCTTATTACGCCAAGGTAAACCTTATAATTTATTGGCACGTATCTGGAAACTTTTAG
ACTTACATGCCGAAGACAAAGTTAAGCGTTCTGAAAAACAACAAGAAGCCTATAAAATTTTAAAGCTACACCCGAAAGGG
ACACAGGAAAATATTCTTAATTTAAGTGGTGTTGAAACAGCCACACTTAAAGTCCTTGAGAAAAAAGACATTGTTCGTTG
TGATTTGGAACCACAAAATGTCACGCCAGAACCTGTGCAATTGGCACAGATGCCCCTCACGCCAAATGACGATCAGAAGA
AAGCTATTCAAGCGATTTTAAAAGCGCAGAAAAAATATCAAGCATTTCTATTGGATGGTTTAACTGGGAGTGGTAAAACC
GAAGTTTATTTGCAAGTAATGCATGAAGTACTTAAACAAGGCAAACAAGTCTTGGTTTTAGTGCCTGAAATCGGCTTAAC
CCCACAAACCATCAGTCGTTTTCAATCACGTTTTCATTGCAATATTGCCTTACTGCACTCAGGCTTAAATGACACTAAAC
GTTTACAAGCATGGCAGCATGCGCAAACGGGCAAAGCCTCTATTATTTTAGGTACACGTTCTGCAATTTATACGCCCTTG
CCACATTTGGGTTTGATTATTTTAGATGAAGAGCATGATCTTTCGTTTAAACAACAAGAAGGCTTTCGCTATCATGCTCG
CGATGTGGCCATGTATCGTGCACATTTAGAAAATTGCCCGATTATTTTAGGTTCGGCCACACCAAGCATCGACAGTTATT
ATCTTGCTCGCCAAGAAAAGCTGACACGGATTGAGTTAAATCAACGTGCTGGTGCCGCAATCATGCCGAAAATGCATTTG
ATTGATTTAAAAGTGGCAAAAAAACAGCATGGCTTAAGTGATGTTTTAATTGCTGAAATCAAAAAACGTTTAGAGAAAAA
AGAACAAGTTCTGATTTTTTTAAATCGACGTGGTTATGCCCCTGTGCTTATCTGTGAAAGCTGTGGTTGGCAGGCCAATT
GCCCCAACTGCGATGCTCACTTTACCCTGCATTTAAAGCCTTATCAGCATTTACATTGTCACCATTGTGGCACGATTAAT
CGTATGCCTGATGCTTGCCCACAATGCAATCAACAACAATTAAAGCCAATTGGCATGGGAACCGCCAAAGTTGAAGAAAC
TTTGAATGAGCTGTTCCCTCACTTCCCCGTCTTGCGCGTAGACCGTGACTCAACCAGTCGGGTTGGCAGTTGGCAAAAGA
TTTATGATCAAATCCAAAAAAGTGAACCGACCATTTTACTTGGCACTCAAATGCTGGCAAAAGGACATCACTTTCCTTAT
GTCACCTTAGTGGCAATTTTGGATATTGATGCAGGTTTGTTGAGTGTGGATTTCCGCGCCACAGAACGTACTGCGCAATT
GATTATTCAAGTTGCAGGCCGTGCGGGTCGTGGTGAAATGAAGGGTGAAGTCTATTTACAAACCCTAAGACCTGATCATC
CGTTATTAAACACCTTGATTCATAATGACTATCGGACCTTTGCCAAACAAACCTTAAAAGATCGCCAAGCAGCACTACTT
CCACCCTTTCGTTATACAGCTTTGATCCGCTGTGATTCAAAGCATCAGGAAGAAAATTTAAAGTTTCTGACTGAAATGAC
GCAGCTGCTTAGACAAACTGCCGAAGAGAATTTAATTGATATTTGGGGGCCTATTCCAGCGCCAATGGAGCGTAAAGCAG
GGCGTTACCAAGCCCATGTGGTTTTGCTTTCTCAAGATCGGGCAAAAATGCATTTTTATATTCGTCAATGGTGGCAACAG
CTGTTACATAGCAAACCCTCTACGATGAAAGTCACCATAGATGTTGATCCGCAAGAGTTGAGTTGA

Domains

Predicted by InterproScan.

Primosomal protein N C-terminal domain (PriA_C)

(642-737)

3DNA-binding domain (3BD) (PriA_3primeBD)

(15-114)

Type III restriction enzyme, res subunit (ResIII)

(207-369)

Helicase conserved C-terminal domain (Helicase_C)

(532-598)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

72.275

100

0.725

Multiple sequence alignment