Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AS4_RS18870 Genome accession   NZ_AP014630
Coordinates   4058724..4059527 (-) Length   267 a.a.
NCBI ID   WP_096735174.1    Uniprot ID   -
Organism   Acinetobacter guillouiae strain NBRC 110550     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4053724..4064527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS4_RS18850 (AS4_38040) - 4054784..4055485 (-) 702 WP_004816992.1 tetratricopeptide repeat protein -
  AS4_RS18855 (AS4_38050) hisS 4055516..4056811 (-) 1296 WP_034612341.1 histidine--tRNA ligase -
  AS4_RS18860 (AS4_38060) ispG 4056808..4057923 (-) 1116 WP_004725885.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  AS4_RS18865 (AS4_38070) - 4057945..4058733 (-) 789 WP_004725882.1 helix-turn-helix domain-containing protein -
  AS4_RS18870 (AS4_38080) pilF 4058724..4059527 (-) 804 WP_096735174.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  AS4_RS18875 (AS4_38090) rlmN 4059593..4060837 (-) 1245 WP_004725880.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  AS4_RS18880 (AS4_38100) ndk 4061016..4061447 (-) 432 WP_004725878.1 nucleoside-diphosphate kinase -
  AS4_RS18885 (AS4_38110) iscX 4061591..4061791 (-) 201 WP_004725877.1 Fe-S cluster assembly protein IscX -
  AS4_RS18890 (AS4_38120) - 4062010..4062561 (-) 552 WP_096735175.1 phosphatase PAP2 family protein -
  AS4_RS18895 (AS4_38130) - 4062593..4063879 (-) 1287 WP_096735176.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 29932.08 Da        Isoelectric Point: 9.2123

>NTDB_id=66374 AS4_RS18870 WP_096735174.1 4058724..4059527(-) (pilF) [Acinetobacter guillouiae strain NBRC 110550]
MYQKAVVILAIGLSALVVQGCQTTNSDTIIKKDPEKAVKVRTQLAAEYIRTGDLDAAKRALDQALETDSRDSAANMMMGV
LLQQEGSKISMEKADGYFKRAISSDSKNAQARNNYGTYLFVSERYNDAIEQLSIAGAALGYDQRYKALENLGRVYLKVGD
VANAEKAFKQALQVNRDSIISLVELSEIFYLQQKSAQASQLYEQYVRLVGQDKQGARALWIGLRIARANNDQMGMQVLVN
QLRALFPDSKEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=66374 AS4_RS18870 WP_096735174.1 4058724..4059527(-) (pilF) [Acinetobacter guillouiae strain NBRC 110550]
ATGTATCAAAAAGCAGTTGTAATATTGGCAATTGGTTTATCTGCATTGGTCGTACAGGGTTGTCAAACGACAAATTCAGA
CACAATAATTAAAAAAGATCCTGAAAAAGCCGTTAAAGTACGTACTCAGCTTGCTGCTGAATATATTCGTACAGGTGATT
TAGATGCTGCAAAACGCGCACTAGACCAAGCTTTAGAAACTGATTCACGTGATTCTGCGGCAAATATGATGATGGGTGTC
TTACTGCAACAAGAAGGCAGTAAGATCAGTATGGAAAAAGCAGACGGCTATTTTAAGCGTGCAATTTCCAGTGACTCAAA
AAATGCACAAGCTCGTAATAATTATGGAACATATTTATTTGTTTCTGAGCGCTATAATGACGCGATTGAACAGTTGAGTA
TAGCTGGTGCTGCGCTTGGTTATGATCAACGTTACAAGGCTTTAGAAAATTTAGGTCGGGTTTACTTAAAAGTGGGTGAT
GTGGCAAATGCTGAAAAAGCGTTCAAACAAGCTTTACAAGTCAATCGCGATTCGATTATTTCTCTGGTAGAGTTATCAGA
AATTTTCTATTTGCAACAAAAAAGTGCGCAAGCAAGTCAACTCTATGAGCAATATGTTCGTTTAGTAGGTCAGGACAAAC
AGGGTGCGAGAGCGCTTTGGATTGGTTTACGTATTGCACGTGCCAATAATGATCAGATGGGAATGCAAGTGTTGGTCAAC
CAACTGCGTGCATTGTTCCCGGACAGTAAAGAATATCAACGTTATCTGCAATTACAGTACAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(40-69)

Tetratricopeptide repeat (TPR_2)

(146-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

74.717

99.251

0.742
  pilF Acinetobacter baylyi ADP1

67.843

95.506

0.648

Multiple sequence alignment