Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MNO09_RS09785 Genome accession   NZ_CP093325
Coordinates   1400425..1400859 (+) Length   144 a.a.
NCBI ID   WP_000811502.1    Uniprot ID   A0A5C5ACH0
Organism   Bacillus sp. N5-665     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1395425..1405859
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNO09_RS09765 (MNO09_09685) - 1396328..1397491 (-) 1164 WP_000434593.1 aminotransferase A -
  MNO09_RS09770 (MNO09_09690) - 1397615..1397815 (-) 201 WP_000929285.1 hypothetical protein -
  MNO09_RS09775 (MNO09_09695) kinB 1398125..1399399 (+) 1275 WP_000420381.1 sporulation sensor histidine kinase KinB -
  MNO09_RS09780 (MNO09_09700) - 1399438..1400187 (-) 750 WP_000388458.1 DUF3967 domain-containing protein -
  MNO09_RS09785 (MNO09_09705) nucA/comI 1400425..1400859 (+) 435 WP_000811502.1 DNA-entry nuclease Machinery gene
  MNO09_RS09790 (MNO09_09710) comJ 1400895..1401284 (+) 390 WP_000424013.1 competence protein ComJ -
  MNO09_RS09795 (MNO09_09715) metE 1401322..1403610 (-) 2289 WP_001007589.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  MNO09_RS09800 (MNO09_09720) - 1404262..1404693 (+) 432 WP_088107350.1 NUDIX domain-containing protein -
  MNO09_RS09805 (MNO09_09725) - 1404885..1405442 (-) 558 WP_000062071.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16010.38 Da        Isoelectric Point: 8.4331

>NTDB_id=663606 MNO09_RS09785 WP_000811502.1 1400425..1400859(+) (nucA/comI) [Bacillus sp. N5-665]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=663606 MNO09_RS09785 WP_000811502.1 1400425..1400859(+) (nucA/comI) [Bacillus sp. N5-665]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTACTTGTTCC
AGAAGAAACAAGTATCAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGGA
AACATATTACCGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCGCATATTGAATATATAAGTCCAGCCGATAATCGCGGGGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACACGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C5ACH0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549