Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   N023_RS21435 Genome accession   NZ_CP093321
Coordinates   4974986..4976353 (-) Length   455 a.a.
NCBI ID   WP_024664007.1    Uniprot ID   A0AAQ1L5G2
Organism   Pseudomonas syringae CC440     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4969986..4981353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N023_RS21415 (N023_21415) yjiA 4970939..4971916 (-) 978 WP_024664009.1 GTPase -
  N023_RS21420 (N023_21420) - 4972012..4972209 (-) 198 WP_003313802.1 YbdD/YjiX family protein -
  N023_RS21425 (N023_21425) - 4972237..4974312 (-) 2076 WP_004416062.1 carbon starvation CstA family protein -
  N023_RS21430 (N023_21430) - 4974597..4974971 (+) 375 WP_024664008.1 PilZ domain-containing protein -
  N023_RS21435 (N023_21435) radA 4974986..4976353 (-) 1368 WP_024664007.1 DNA repair protein RadA Machinery gene
  N023_RS21440 (N023_21440) mscL 4976550..4976996 (+) 447 WP_003313807.1 large-conductance mechanosensitive channel protein MscL -
  N023_RS21445 (N023_21445) - 4977437..4978213 (-) 777 WP_024664034.1 ferredoxin--NADP reductase -
  N023_RS21450 (N023_21450) - 4978550..4978783 (+) 234 WP_003340758.1 helix-turn-helix transcriptional regulator -
  N023_RS21455 (N023_21455) - 4978914..4980038 (+) 1125 WP_024664035.1 methyltransferase -
  N023_RS21460 (N023_21460) - 4980131..4980298 (-) 168 WP_002555239.1 DUF2474 domain-containing protein -
  N023_RS21465 (N023_21465) cydB 4980320..4981327 (-) 1008 WP_003396217.1 cytochrome d ubiquinol oxidase subunit II -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48883.45 Da        Isoelectric Point: 7.1316

>NTDB_id=663540 N023_RS21435 WP_024664007.1 4974986..4976353(-) (radA) [Pseudomonas syringae CC440]
MAKAKRLYGCTECGATFPKWAGQCGECGAWNTLVETMIESGAAAPPTGRTGWTGSQAQIRTLAEVSVEEIPRFSTNSTEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAQRMPALYVTGEESQQQVAMRARRLGLPQDKLRVMTETCIETIIA
TAKVEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHMS
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=663540 N023_RS21435 WP_024664007.1 4974986..4976353(-) (radA) [Pseudomonas syringae CC440]
ATGGCCAAGGCCAAACGCTTGTACGGCTGCACCGAGTGCGGCGCGACATTTCCCAAGTGGGCGGGCCAGTGTGGCGAATG
CGGTGCCTGGAACACACTGGTCGAGACCATGATTGAAAGCGGTGCCGCCGCGCCGCCCACCGGGCGTACCGGCTGGACCG
GTTCGCAGGCGCAGATCAGGACCCTGGCGGAGGTCAGCGTCGAAGAAATCCCGCGCTTCTCCACCAACTCCACCGAGCTG
GACAGGGTACTCGGCGGCGGTCTGGTTGACGGTTCGGTGGTTCTGATTGGCGGCGACCCCGGCATCGGCAAGTCAACGAT
CCTCTTGCAGACGCTGTGCAACATCGCCCAGCGCATGCCTGCGCTGTATGTCACCGGTGAGGAATCCCAACAGCAGGTTG
CCATGCGTGCCCGGCGCCTGGGTCTGCCGCAGGACAAGCTGCGGGTCATGACCGAAACCTGCATCGAGACCATCATTGCC
ACTGCCAAGGTCGAGAAGCCCAAGGTGATGGTGATCGATTCGATCCAGACGATTTTCACCGAACAGCTGCAGTCGGCCCC
TGGCGGCGTGTCGCAGGTTCGTGAAAGCGCCGCCTTGCTGGTGCGTTACGCCAAGCAGAGCGGCACGGCGATCTTCCTGG
TCGGCCACGTCACCAAGGAAGGCGCCCTGGCCGGTCCGCGTGTGCTTGAGCACATGGTCGACACCGTGCTGTATTTCGAG
GGCGAGTCGGATGGTCGCCTGCGCCTGTTACGAGCGGTGAAGAACCGCTTTGGCGCGGTCAATGAGCTGGGCGTGTTCGG
CATGACCGACAAGGGCCTGAAAGAAGTCTCCAACCCGTCGGCGATCTTTCTCACTCGTGCGCAGGAAGAAGTGCCAGGCA
GTGTGGTCATGGCCACCTGGGAAGGCACGCGGCCGATGCTGGTGGAAGTGCAGGCGCTGGTGGACGACAGTCACATGTCC
AACCCGCGCCGCGTAACGCTGGGACTGGATCAGAATCGTCTGGCGATGCTGCTGGCAGTCCTGCACCGTCACGGTGGCAT
TCCTACTCACGATCAGGACGTGTTCCTCAACGTGGTAGGCGGCGTGAAAGTGCTGGAAACCGCCTCCGACCTGGCCTTGA
TGGCGGCGGTCATGTCCAGCCTGCGCAATCGTCCGCTGCCGCATGACTTGCTGGTGTTCGGTGAGGTGGGGCTTTCCGGT
GAAGTCAGGCCGGTGCCCAGCGGCCAGGAGCGCCTCAAGGAGGCCGCCAAGCACGGCTTCAAGCGCGCCATCGTGCCCAA
GGGCAACGCGCCGAAAGAAGCGCCGCCCGGCTTGCAGATCATTGCCGTTACGCGACTTGAACAGGCCCTCGATGCGCTGT
TCGAGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(73-221)

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.123

100

0.492
  radA Streptococcus pneumoniae Rx1

46.739

100

0.473
  radA Streptococcus pneumoniae R6

46.739

100

0.473
  radA Streptococcus pneumoniae TIGR4

46.739

100

0.473
  radA Streptococcus pneumoniae D39

46.739

100

0.473
  radA Streptococcus mitis SK321

46.93

100

0.47
  radA Streptococcus mitis NCTC 12261

46.711

100

0.468