Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MM326_RS00650 Genome accession   NZ_CP093302
Coordinates   129411..130784 (+) Length   457 a.a.
NCBI ID   WP_255224351.1    Uniprot ID   -
Organism   Alkalihalobacillus sp. LMS6     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 124411..135784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MM326_RS00630 (MM326_00630) - 124719..125189 (+) 471 WP_099305521.1 CtsR family transcriptional regulator -
  MM326_RS00635 (MM326_00635) - 125231..125752 (+) 522 WP_306345964.1 UvrB/UvrC motif-containing protein -
  MM326_RS00640 (MM326_00640) - 125749..126822 (+) 1074 WP_255224350.1 protein arginine kinase -
  MM326_RS00645 (MM326_00645) clpC 126851..129304 (+) 2454 WP_099305515.1 ATP-dependent protease ATP-binding subunit ClpC -
  MM326_RS00650 (MM326_00650) radA 129411..130784 (+) 1374 WP_255224351.1 DNA repair protein RadA Machinery gene
  MM326_RS00655 (MM326_00655) disA 130791..131864 (+) 1074 WP_099305511.1 DNA integrity scanning diadenylate cyclase DisA -
  MM326_RS00660 (MM326_00660) - 132017..133123 (+) 1107 WP_099305509.1 PIN/TRAM domain-containing protein -
  MM326_RS00665 (MM326_00665) ispD 133222..133935 (+) 714 WP_099305507.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MM326_RS00670 (MM326_00670) ispF 133910..134386 (+) 477 WP_141556832.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49760.46 Da        Isoelectric Point: 7.0295

>NTDB_id=663355 MM326_RS00650 WP_255224351.1 129411..130784(+) (radA) [Alkalihalobacillus sp. LMS6]
MAKAKTKFVCQECGYESAKWMGKCPGCQSWNTLVEEKAVQEKQRSRSFVTSGATIHKAQPITSVVSEKEPRVSTTMTELN
RVLGGGIVPGSLVLVGGDPGIGKSTLLLQLSAKLAKTKERVLYISGEESVKQTKLRADRLELSSEDLYVLAETDVSLIEQ
AIDQVQPSLVIIDSIQTVYQEELTSAPGSVAQVRECTATFMRIAKTRGTAVFIVGHVTKQGSIAGPKMLEHMVDSVLYFE
GERHHTYRILRAVKNRFGSTNEMGIFEMKELGLEEVLNPSEIFLEERSQGAAGSVVVASMEGTRPVLVELQALISPMGFG
NPRRMATGLDHNRVSLLMAVLEKRIGMLLQNQDAYLKVAGGVKLDEPAVDLAIALSIASSFRNQPTEPMDVAIGEVGLTG
EIRRVSRIEQRVNEAAKLGFKRVIIPEKNLGGWTTPEGIEVVSVPTLDKALDVALGR

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=663355 MM326_RS00650 WP_255224351.1 129411..130784(+) (radA) [Alkalihalobacillus sp. LMS6]
ATGGCAAAAGCAAAAACGAAATTTGTCTGTCAAGAGTGCGGATATGAATCAGCGAAATGGATGGGCAAGTGTCCAGGGTG
CCAAAGCTGGAATACATTAGTGGAAGAAAAAGCTGTTCAAGAAAAGCAACGATCAAGAAGTTTTGTCACATCAGGTGCAA
CCATTCATAAAGCCCAACCGATAACAAGCGTAGTAAGTGAGAAGGAACCACGTGTAAGTACGACGATGACGGAGTTGAAT
CGTGTGCTAGGTGGGGGAATTGTCCCAGGTTCACTTGTCTTGGTAGGAGGCGATCCGGGAATCGGAAAATCAACACTTTT
ATTGCAATTATCTGCGAAGTTAGCAAAAACAAAAGAGCGGGTACTTTATATTTCAGGAGAAGAGTCTGTAAAGCAGACGA
AGCTGAGAGCGGATCGACTAGAGCTCTCGTCAGAAGATCTTTACGTACTTGCGGAAACAGATGTATCGCTCATTGAACAA
GCGATCGATCAAGTACAGCCTTCTTTAGTAATCATTGATTCTATTCAAACCGTCTATCAAGAAGAGTTGACTTCAGCACC
TGGAAGTGTTGCTCAAGTTCGAGAGTGCACAGCTACGTTCATGCGCATTGCAAAAACGAGAGGTACGGCTGTTTTTATTG
TTGGTCACGTAACGAAGCAAGGCTCCATTGCAGGGCCTAAAATGCTCGAACATATGGTTGATTCCGTATTGTACTTTGAA
GGGGAGCGCCATCATACGTATAGAATTCTTCGAGCGGTAAAAAACCGTTTTGGTTCAACGAATGAAATGGGTATTTTTGA
AATGAAGGAATTAGGTCTAGAAGAAGTGCTAAATCCATCAGAAATCTTTCTGGAGGAGCGTTCACAAGGTGCTGCAGGTT
CAGTGGTTGTGGCTTCAATGGAAGGGACAAGACCTGTTCTCGTGGAGTTACAGGCATTAATTTCACCTATGGGATTCGGA
AATCCGAGACGAATGGCTACTGGATTGGATCATAATCGTGTGTCACTGTTAATGGCGGTCTTAGAAAAAAGAATTGGCAT
GCTGTTACAAAATCAAGATGCGTATTTAAAAGTGGCTGGAGGTGTGAAGCTGGATGAGCCGGCCGTTGATTTAGCCATTG
CACTAAGTATTGCCTCTAGCTTTCGGAATCAACCAACAGAGCCAATGGATGTTGCAATTGGAGAAGTAGGTTTAACAGGT
GAAATTAGACGGGTATCTAGAATAGAACAGAGAGTAAATGAAGCGGCGAAGCTAGGCTTTAAGCGCGTTATTATACCAGA
GAAGAACTTAGGTGGTTGGACGACGCCTGAGGGTATTGAAGTAGTAAGCGTGCCGACGCTTGATAAAGCACTCGATGTTG
CTTTAGGGAGGTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-221)

Subunit ChlI of Mg-chelatase (ChlI)

(363-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

72.21

100

0.722
  radA Streptococcus pneumoniae Rx1

60.31

98.687

0.595
  radA Streptococcus pneumoniae D39

60.31

98.687

0.595
  radA Streptococcus pneumoniae R6

60.31

98.687

0.595
  radA Streptococcus pneumoniae TIGR4

60.31

98.687

0.595
  radA Streptococcus mitis NCTC 12261

60.31

98.687

0.595
  radA Streptococcus mitis SK321

60.31

98.687

0.595