Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MM300_RS09540 Genome accession   NZ_CP093301
Coordinates   2056530..2057936 (+) Length   468 a.a.
NCBI ID   WP_255244845.1    Uniprot ID   -
Organism   Evansella sp. LMS18     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2051530..2062936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MM300_RS09520 (MM300_09495) - 2051812..2052288 (+) 477 WP_255244841.1 CtsR family transcriptional regulator -
  MM300_RS09525 (MM300_09500) - 2052302..2052832 (+) 531 WP_255244842.1 UvrB/UvrC motif-containing protein -
  MM300_RS09530 (MM300_09505) - 2052835..2053905 (+) 1071 WP_255244843.1 protein arginine kinase -
  MM300_RS09535 (MM300_09510) clpC 2053930..2056377 (+) 2448 WP_255244844.1 ATP-dependent protease ATP-binding subunit ClpC -
  MM300_RS09540 (MM300_09515) radA 2056530..2057936 (+) 1407 WP_255244845.1 DNA repair protein RadA Machinery gene
  MM300_RS09545 (MM300_09520) disA 2057911..2058993 (+) 1083 WP_255244846.1 DNA integrity scanning diadenylate cyclase DisA -
  MM300_RS09550 (MM300_09525) - 2059193..2060290 (+) 1098 WP_255244847.1 PIN/TRAM domain-containing protein -
  MM300_RS09555 (MM300_09530) ispD 2060478..2061173 (+) 696 WP_255244848.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MM300_RS09560 (MM300_09535) ispF 2061170..2061649 (+) 480 WP_255244849.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 468 a.a.        Molecular weight: 50871.33 Da        Isoelectric Point: 6.5981

>NTDB_id=663329 MM300_RS09540 WP_255244845.1 2056530..2057936(+) (radA) [Evansella sp. LMS18]
MAKRKTKFVCQECGYESTKWMGKCPGCQNWNTLVEEFEAAPAEKRRGFVTSGTTASSKPQPITKIERKDERRFDTHINEL
NRVLGGGIVPGSLVLVGGDPGIGKSTLLLQVSAEIAKHGKRVLYISGEESVKQTKLRADRLNISTDELFVLAETDVEHIE
KVIEEINPTLVVIDSIQTVYLDHVTSAPGSVAQVRESTAAFMRIAKTKGIAIFIVGHVTKQGSIAGPRLLEHMVDSVLYF
EGERHHTYRILRAVKNRFGSTNEMGIFEMKEVGLEEVLNPSEIFLEERSAGAAGSAVVASMEGTRTVLVEIQSLISPTSF
GNPRRMATGIDHNRISLIMAVLEKRVGMMLQNQDAYVKVAGGVRLDEPSIDLAIAVAIASSFRDAPAPPADVIIGEVGLT
GEVRRVSRIEQRVSEASKLGFKRVIVPERNLGGWDIPDGIEVAGVNTVSEALEITIGGASSGSPRFEL

Nucleotide


Download         Length: 1407 bp        

>NTDB_id=663329 MM300_RS09540 WP_255244845.1 2056530..2057936(+) (radA) [Evansella sp. LMS18]
ATGGCAAAGAGAAAAACAAAGTTTGTGTGTCAGGAATGCGGCTATGAATCAACGAAGTGGATGGGGAAATGCCCAGGCTG
CCAGAACTGGAACACCCTGGTAGAAGAATTTGAAGCTGCTCCTGCTGAGAAAAGGCGGGGATTTGTGACCTCAGGGACAA
CAGCTTCTTCCAAACCTCAGCCGATTACAAAGATTGAGCGTAAGGATGAGCGGAGGTTCGACACACATATCAATGAATTA
AACCGTGTTCTGGGCGGCGGGATAGTACCTGGCTCCCTGGTTTTAGTCGGCGGCGATCCGGGAATTGGTAAATCAACCCT
TCTGTTACAGGTTTCTGCGGAAATAGCGAAGCATGGAAAAAGAGTATTGTACATATCAGGCGAGGAATCTGTTAAACAGA
CAAAACTCCGTGCTGACAGATTAAATATATCAACGGACGAACTTTTCGTACTTGCAGAAACAGACGTGGAACATATTGAA
AAAGTCATTGAAGAAATAAACCCAACGCTTGTTGTAATAGATTCAATACAGACAGTCTATCTTGACCATGTCACAAGTGC
GCCAGGCAGCGTCGCCCAGGTAAGGGAGTCAACAGCTGCTTTCATGCGTATCGCCAAAACAAAAGGAATCGCAATCTTTA
TAGTTGGGCATGTTACAAAGCAAGGTTCTATCGCAGGCCCAAGGCTCCTGGAACATATGGTTGATTCCGTCCTGTATTTC
GAGGGAGAAAGACATCATACATATCGCATTCTCCGGGCGGTAAAAAACCGGTTTGGCTCCACAAATGAAATGGGCATTTT
TGAAATGAAGGAAGTGGGCCTGGAAGAAGTGCTGAATCCTTCTGAGATTTTTCTGGAAGAACGTTCTGCCGGAGCGGCTG
GCTCTGCGGTAGTAGCTTCTATGGAGGGAACCAGAACCGTTCTCGTTGAAATTCAGTCGCTCATATCCCCTACCAGCTTT
GGTAATCCCCGGCGTATGGCAACTGGAATTGACCACAATCGTATCTCTCTGATTATGGCGGTTCTTGAAAAACGAGTTGG
AATGATGTTGCAAAATCAGGATGCCTATGTAAAAGTTGCCGGAGGAGTTAGGCTTGATGAGCCTTCAATAGATTTAGCCA
TCGCTGTAGCCATTGCCTCCAGTTTCCGGGACGCTCCTGCTCCTCCTGCAGATGTAATTATCGGGGAAGTAGGACTTACA
GGTGAGGTGCGCCGTGTTTCCAGGATAGAGCAGCGTGTCTCAGAAGCAAGCAAACTTGGTTTTAAGCGGGTGATTGTTCC
GGAGAGAAACCTTGGGGGTTGGGATATTCCGGACGGGATTGAAGTTGCCGGAGTGAATACAGTAAGCGAAGCATTAGAGA
TAACGATAGGAGGGGCATCAAGTGGATCACCGAGATTCGAATTATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(347-430)

AAA domain (AAA_25)

(64-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.074

98.077

0.726
  radA Streptococcus pneumoniae Rx1

59.161

96.795

0.573
  radA Streptococcus pneumoniae D39

59.161

96.795

0.573
  radA Streptococcus pneumoniae R6

59.161

96.795

0.573
  radA Streptococcus pneumoniae TIGR4

59.161

96.795

0.573
  radA Streptococcus mitis NCTC 12261

59.161

96.795

0.573
  radA Streptococcus mitis SK321

59.161

96.795

0.573