Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MM271_RS00565 Genome accession   NZ_CP093300
Coordinates   110589..111968 (+) Length   459 a.a.
NCBI ID   WP_243530433.1    Uniprot ID   -
Organism   Alkalihalobacillus sp. LMS39     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 105589..116968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MM271_RS00545 (MM271_00545) - 105900..106382 (+) 483 WP_243530429.1 CtsR family transcriptional regulator -
  MM271_RS00550 (MM271_00550) - 106393..106920 (+) 528 WP_243530430.1 UvrB/UvrC motif-containing protein -
  MM271_RS00555 (MM271_00555) - 106924..107994 (+) 1071 WP_243530431.1 protein arginine kinase -
  MM271_RS00560 (MM271_00560) clpC 108017..110455 (+) 2439 WP_243530432.1 ATP-dependent protease ATP-binding subunit ClpC -
  MM271_RS00565 (MM271_00565) radA 110589..111968 (+) 1380 WP_243530433.1 DNA repair protein RadA Machinery gene
  MM271_RS00570 (MM271_00570) disA 111972..113045 (+) 1074 WP_243530434.1 DNA integrity scanning diadenylate cyclase DisA -
  MM271_RS00575 (MM271_00575) - 113238..114329 (+) 1092 WP_243530435.1 PIN/TRAM domain-containing protein -
  MM271_RS00580 (MM271_00580) ispD 114351..115040 (+) 690 WP_243530436.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MM271_RS00585 (MM271_00585) ispF 115037..115513 (+) 477 WP_243530437.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49935.39 Da        Isoelectric Point: 6.2755

>NTDB_id=663267 MM271_RS00565 WP_243530433.1 110589..111968(+) (radA) [Alkalihalobacillus sp. LMS39]
MAKKKTKFVCQECGYESTKWMGKCPSCSSWNSMVEEWEETKSDSRSRSFVTGNSVAITSPQPITNVKSEEEPRIQTGMKE
LNRVLGGGIVPGSLVLVGGDPGIGKSTILLQTSALLAKMNHKVLYISGEESVKQTKLRADRLQASADLLYVLAETDLDLI
DKAIREITPTLVIIDSIQTVYQAEITSAPGSVSQVRECTAAFMKIAKTTGTAIFIVGHVTKQGSIAGPKLLEHMVDSVLY
FEGERHHTYRILRAVKNRFGSTNEIGIFEMKEEGLEEVLNPSEIFLEERTEGAIGSTVVASMEGTRPVLVEIQALVSPTS
FGNPRRMATGIDHNRVSLLMAVLEKKVGMLLQNQDAYLNVAGGVRLDEPAIDLAITVSIASSFRNQSTNPTDVVIGEIGL
TGEVRRVSRIEQRVNEAAKLGFKRAIIPEKNIGGWSFPKDIKIIGVSTVDDALDVILGG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=663267 MM271_RS00565 WP_243530433.1 110589..111968(+) (radA) [Alkalihalobacillus sp. LMS39]
ATGGCGAAAAAGAAAACAAAATTTGTTTGCCAGGAGTGTGGATATGAATCCACAAAATGGATGGGGAAATGTCCGAGCTG
TTCAAGTTGGAATTCAATGGTTGAAGAATGGGAAGAAACAAAATCAGACTCAAGAAGTCGGTCGTTTGTTACGGGGAACT
CGGTTGCGATTACGAGTCCACAGCCAATTACAAATGTAAAAAGTGAAGAAGAACCACGAATTCAAACAGGAATGAAAGAA
CTAAACCGAGTTCTTGGTGGTGGTATTGTTCCAGGATCTTTAGTTTTAGTTGGAGGAGATCCGGGAATAGGAAAGTCTAC
CATACTTTTGCAAACCTCGGCCTTGTTAGCAAAAATGAATCATAAAGTATTATATATATCAGGTGAAGAGTCCGTAAAAC
AAACGAAGTTACGTGCGGATCGTTTACAAGCCTCAGCTGACTTATTATATGTTTTAGCAGAAACAGACTTAGATTTAATA
GATAAGGCGATAAGAGAGATTACACCTACTCTCGTTATTATTGATTCCATTCAAACTGTATACCAAGCAGAAATTACATC
TGCACCAGGAAGTGTTTCCCAAGTACGAGAGTGTACAGCCGCATTTATGAAAATTGCAAAAACTACAGGGACGGCAATTT
TTATTGTCGGACACGTAACAAAGCAAGGCTCGATTGCTGGTCCAAAGCTACTCGAGCATATGGTGGATTCAGTTTTATAT
TTTGAGGGAGAGCGACATCATACATACCGTATTTTGCGAGCTGTTAAAAACCGCTTCGGTTCCACAAATGAAATCGGAAT
ATTTGAGATGAAAGAGGAAGGATTAGAAGAGGTATTAAACCCTTCAGAGATCTTTTTAGAAGAGCGGACAGAGGGTGCTA
TTGGTTCAACTGTAGTGGCCTCGATGGAAGGAACTCGTCCTGTGCTTGTAGAAATCCAAGCGTTAGTTTCTCCAACAAGC
TTTGGAAACCCGAGAAGAATGGCAACAGGAATTGACCATAACCGTGTTTCTTTATTGATGGCTGTATTAGAAAAAAAAGT
GGGGATGCTATTACAAAATCAAGATGCTTACTTAAATGTTGCAGGGGGAGTCAGGTTAGATGAACCTGCGATCGATTTAG
CTATAACGGTAAGTATCGCTTCGAGTTTTCGAAATCAATCTACAAACCCAACCGATGTGGTTATAGGTGAAATCGGATTA
ACAGGTGAAGTCAGAAGAGTGTCTCGTATTGAACAAAGAGTGAATGAAGCTGCAAAGCTTGGTTTTAAGCGCGCCATTAT
CCCGGAAAAAAACATCGGAGGCTGGTCATTCCCAAAAGATATAAAAATCATCGGTGTATCAACAGTAGATGATGCATTAG
ACGTAATACTAGGAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-223)

Subunit ChlI of Mg-chelatase (ChlI)

(347-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

71.895

100

0.719
  radA Streptococcus pneumoniae Rx1

59.956

99.564

0.597
  radA Streptococcus pneumoniae D39

59.956

99.564

0.597
  radA Streptococcus pneumoniae R6

59.956

99.564

0.597
  radA Streptococcus pneumoniae TIGR4

59.956

99.564

0.597
  radA Streptococcus mitis NCTC 12261

59.956

99.564

0.597
  radA Streptococcus mitis SK321

59.737

99.564

0.595