Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MNY35_RS10795 Genome accession   NZ_CP093298
Coordinates   2289101..2290480 (-) Length   459 a.a.
NCBI ID   WP_014304212.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain MN-13     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2284101..2295480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNY35_RS10775 (MNY35_10775) ispF 2285621..2286097 (-) 477 WP_003156407.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  MNY35_RS10780 (MNY35_10780) ispD 2286090..2286788 (-) 699 WP_015239038.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MNY35_RS10785 (MNY35_10785) - 2286801..2287901 (-) 1101 WP_003156403.1 PIN/TRAM domain-containing protein -
  MNY35_RS10790 (MNY35_10790) disA 2288015..2289097 (-) 1083 WP_033575238.1 DNA integrity scanning diadenylate cyclase DisA -
  MNY35_RS10795 (MNY35_10795) radA 2289101..2290480 (-) 1380 WP_014304212.1 DNA repair protein RadA Machinery gene
  MNY35_RS10800 (MNY35_10800) clpC 2290574..2293006 (-) 2433 WP_007410388.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  MNY35_RS10805 (MNY35_10805) - 2293003..2294094 (-) 1092 WP_015416670.1 protein arginine kinase -
  MNY35_RS10810 (MNY35_10810) - 2294094..2294651 (-) 558 WP_007410387.1 UvrB/UvrC motif-containing protein -
  MNY35_RS10815 (MNY35_10815) ctsR 2294665..2295129 (-) 465 WP_003156396.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49538.97 Da        Isoelectric Point: 7.1316

>NTDB_id=663180 MNY35_RS10795 WP_014304212.1 2289101..2290480(-) (radA) [Bacillus amyloliquefaciens strain MN-13]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVQTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQMLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPEANADGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=663180 MNY35_RS10795 WP_014304212.1 2289101..2290480(-) (radA) [Bacillus amyloliquefaciens strain MN-13]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAATGGATGGGAAAGTGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCAGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCAGAAGAACCCCGCGTCCAAACAAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTGAAAGGCTCGTTAGTATTAATCGGCGGCGATCCCGGGATCGGAAAATCAACGCT
CCTTCTCCAAGTATCCGCACAATTAGCAGATACGGCCGGCAGTGTGCTTTACATTTCAGGGGAAGAATCCGTGAAGCAAA
CGAAGCTGCGCGCCGACAGACTTGGAATCAACAGCCAAATGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTTTATCAAAGTGATATCACCTCGGC
TCCCGGCAGTGTATCACAAGTAAGAGAATGTACGGCTGAATTGATGAAAATCGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGGCACGTCACAAAAGAAGGATCGATCGCAGGGCCGAGATTGCTTGAGCACATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCACCATACATTCCGTATTTTGCGGGCCGTAAAAAACCGTTTCGGCTCCACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAGGGGCTTACGGAAGTGCTGAACCCTTCTGAAATTTTCTTGGAAGAGCGTTCTGCCGGGGCATCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGAACGAGACCGATTCTGGTTGAAATCCAGGCGCTCATTTCACCGACCAGTTTT
GGAAACCCGCGCCGCATGGCTACAGGCATTGACCACAACAGGGTATCGTTAATTATGGCTGTGCTTGAGAAGCGGGTGGG
TCTTTTGCTTCAAAATCAAGATGCGTATTTAAAGGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCCTCAAGCTTCAGAGATACACCGCCGAATCCCGCAGATTGCTTTATCGGTGAAGTGGGTCTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAGGAAGCGGCGAAACTCGGCTTCAAGCGCATGATCATACC
TGAGGCGAATGCAGACGGGTGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(71-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

93.45

99.782

0.932
  radA Streptococcus pneumoniae Rx1

63.797

98.693

0.63
  radA Streptococcus pneumoniae D39

63.797

98.693

0.63
  radA Streptococcus pneumoniae R6

63.797

98.693

0.63
  radA Streptococcus pneumoniae TIGR4

63.797

98.693

0.63
  radA Streptococcus mitis NCTC 12261

63.797

98.693

0.63
  radA Streptococcus mitis SK321

63.576

98.693

0.627