Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MNY35_RS09560 Genome accession   NZ_CP093298
Coordinates   2025969..2026406 (+) Length   145 a.a.
NCBI ID   WP_015416783.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain MN-13     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2020969..2031406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNY35_RS09545 (MNY35_09545) - 2023828..2024190 (-) 363 WP_014304320.1 helix-turn-helix domain-containing protein -
  MNY35_RS09550 (MNY35_09550) hxlA 2024422..2025057 (+) 636 WP_015416785.1 3-hexulose-6-phosphate synthase -
  MNY35_RS09555 (MNY35_09555) hxlB 2025054..2025611 (+) 558 WP_015416784.1 6-phospho-3-hexuloisomerase -
  MNY35_RS09560 (MNY35_09560) nucA/comI 2025969..2026406 (+) 438 WP_015416783.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MNY35_RS09565 (MNY35_09565) nin/comJ 2026427..2026825 (+) 399 WP_015416782.1 competence protein ComJ Regulator
  MNY35_RS09570 (MNY35_09570) - 2026866..2028302 (-) 1437 WP_015416781.1 family 1 glycosylhydrolase -
  MNY35_RS09575 (MNY35_09575) - 2028406..2028729 (-) 324 WP_007409357.1 YckD family protein -
  MNY35_RS09580 (MNY35_09580) - 2028801..2029235 (-) 435 WP_033575151.1 RDD family protein -
  MNY35_RS09585 (MNY35_09585) - 2029250..2030386 (-) 1137 WP_015416779.1 zinc-dependent alcohol dehydrogenase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16038.34 Da        Isoelectric Point: 8.4850

>NTDB_id=663177 MNY35_RS09560 WP_015416783.1 2025969..2026406(+) (nucA/comI) [Bacillus amyloliquefaciens strain MN-13]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLLKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=663177 MNY35_RS09560 WP_015416783.1 2025969..2026406(+) (nucA/comI) [Bacillus amyloliquefaciens strain MN-13]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACATCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTGAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-132)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.016

86.897

0.634