Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MN093_RS08000 Genome accession   NZ_CP093291
Coordinates   1493130..1494479 (-) Length   449 a.a.
NCBI ID   WP_215576676.1    Uniprot ID   -
Organism   Bacillus mycoides strain DSM 2048     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1488130..1499479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MN093_RS07970 (MN093_07970) - 1488773..1489468 (-) 696 WP_060749253.1 hypothetical protein -
  MN093_RS07975 (MN093_07975) - 1489455..1490243 (-) 789 WP_215576674.1 hypothetical protein -
  MN093_RS07980 (MN093_07980) - 1490275..1490859 (-) 585 WP_002068272.1 hypothetical protein -
  MN093_RS07985 (MN093_07985) hpf 1491238..1491780 (-) 543 WP_002016135.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  MN093_RS07990 (MN093_07990) cspC 1492102..1492299 (-) 198 WP_001990088.1 cold shock protein CspC -
  MN093_RS07995 (MN093_07995) - 1492426..1493130 (-) 705 WP_215576675.1 ComF family protein -
  MN093_RS08000 (MN093_08000) comFA 1493130..1494479 (-) 1350 WP_215576676.1 ATP-dependent helicase ComFA Machinery gene
  MN093_RS08005 (MN093_08005) - 1494606..1495922 (-) 1317 WP_215576677.1 NlpC/P60 family protein -
  MN093_RS08010 (MN093_08010) - 1496187..1496501 (-) 315 WP_002089608.1 winged helix-turn-helix transcriptional regulator -
  MN093_RS08015 (MN093_08015) - 1496664..1497506 (-) 843 WP_002016143.1 DegV family protein -
  MN093_RS08020 (MN093_08020) - 1497898..1498533 (+) 636 WP_131246517.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 50807.16 Da        Isoelectric Point: 9.5255

>NTDB_id=663117 MN093_RS08000 WP_215576676.1 1493130..1494479(-) (comFA) [Bacillus mycoides strain DSM 2048]
MLAGKQLLLEELSSDLQDKLDHLKKNREVVCVQGVIKKSSKYMCQRCGNIEQRLFASFLCKRCNKVCTYCRKCITMGRVS
ECVVLVRGIAEISGENYLNPLQWEGVLSAGQELAAQSVVDAVKQKESFFIWAVCGAGKTEMLFRGIAEALQKGERVCIAT
PRTDVVLELAPRLQEVFPNINVAALYGGSLDREKDAALIVATTHQLLRYYRAFHVMIVDEIDAFPYHADKMLHYAVNEAT
KEEAARIYLTATPDEKWKRKLRNGKQKGVIISGRYHRHPLPVPAFRWCGNWKKGLIRKKIPRALLQWLNLYLSKRYPIFL
FVPHVRYVEEISQLLKSLNNQIDGVHAEDPARKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSSDEPHGDVIYFHYGKTEAMVRAKKHIQSMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=663117 MN093_RS08000 WP_215576676.1 1493130..1494479(-) (comFA) [Bacillus mycoides strain DSM 2048]
ATGTTAGCCGGGAAACAGTTACTATTAGAAGAACTTTCTTCAGACTTGCAGGATAAATTGGATCATTTGAAAAAGAATCG
AGAAGTCGTTTGTGTACAAGGGGTAATTAAGAAATCTTCAAAATATATGTGCCAGCGTTGCGGAAATATAGAGCAGCGAC
TGTTTGCATCATTTCTATGTAAAAGGTGCAATAAAGTATGTACGTATTGCCGGAAATGTATAACGATGGGCAGGGTAAGT
GAATGTGTTGTACTTGTTCGCGGGATTGCTGAAATAAGCGGAGAAAATTATTTAAATCCGTTACAGTGGGAAGGCGTTTT
GTCTGCTGGTCAGGAGTTAGCTGCGCAAAGTGTCGTTGACGCTGTTAAGCAGAAAGAATCATTTTTTATTTGGGCGGTGT
GCGGGGCTGGAAAAACAGAAATGTTGTTTCGCGGAATTGCAGAGGCACTACAAAAGGGAGAGAGAGTTTGTATTGCAACG
CCGAGAACGGACGTTGTACTTGAATTAGCACCAAGATTACAAGAAGTGTTTCCAAATATAAATGTAGCTGCTTTATACGG
AGGGAGTTTAGACCGAGAAAAAGATGCGGCGTTAATCGTTGCAACTACACATCAATTGTTACGTTATTATAGGGCGTTCC
ATGTCATGATTGTAGATGAGATAGATGCTTTTCCATATCATGCGGATAAGATGTTACATTATGCAGTAAACGAAGCGACG
AAAGAGGAGGCAGCACGTATTTATTTAACTGCAACTCCAGATGAAAAGTGGAAGCGTAAATTGCGGAATGGTAAGCAAAA
AGGGGTTATTATTTCAGGACGTTATCATCGTCATCCGTTGCCAGTTCCAGCATTTCGATGGTGCGGAAATTGGAAAAAGG
GTCTTATACGTAAAAAAATTCCTCGTGCTTTATTACAATGGTTAAATCTGTACTTATCGAAACGATACCCCATTTTTCTA
TTTGTTCCTCATGTGCGATATGTAGAAGAAATTAGCCAGTTATTAAAATCATTAAACAATCAAATTGACGGTGTGCATGC
AGAAGATCCGGCGAGAAAAGAGAAGGTAGCAGCTTTCAGAAAAGGAGAAATCCCTTTATTAGTTACAACGACGATTTTGG
AACGAGGAGTAACTGTTAAAAATTTACAAGTTGCAGTTTTAGGAGCTGAGGAAGAGATATTTTCAGAAAGTGCTCTCGTT
CAAATTGCAGGGCGTGCTGGCAGAAGCTCTGATGAACCGCATGGAGATGTTATCTATTTTCATTATGGAAAGACAGAGGC
GATGGTGCGTGCGAAAAAACATATTCAAAGTATGAATAAGAATGCTAAAGAACAAGGATTGATAGATTGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(107-254)

Helicase conserved C-terminal domain (Helicase_C)

(313-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

50.905

98.441

0.501
  comFA Latilactobacillus sakei subsp. sakei 23K

41.206

88.641

0.365