Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MNU32_RS12245 Genome accession   NZ_CP093212
Coordinates   2519751..2521124 (-) Length   457 a.a.
NCBI ID   WP_002445730.1    Uniprot ID   A0A4Y7VQ23
Organism   Staphylococcus epidermidis strain TSM-51     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2514751..2526124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNU32_RS12230 (MNU32_12230) cysE 2515772..2516401 (-) 630 WP_002438669.1 serine O-acetyltransferase -
  MNU32_RS12235 (MNU32_12235) gltX 2516786..2518240 (-) 1455 WP_002438667.1 glutamate--tRNA ligase -
  MNU32_RS12240 (MNU32_12240) - 2518639..2519697 (-) 1059 WP_001832313.1 PIN/TRAM domain-containing protein -
  MNU32_RS12245 (MNU32_12245) radA 2519751..2521124 (-) 1374 WP_002445730.1 DNA repair protein RadA Machinery gene
  MNU32_RS12250 (MNU32_12250) - 2521615..2524068 (-) 2454 WP_002445729.1 ATP-dependent Clp protease ATP-binding subunit -
  MNU32_RS12255 (MNU32_12255) - 2524082..2525089 (-) 1008 WP_002438663.1 protein arginine kinase -
  MNU32_RS12260 (MNU32_12260) - 2525079..2525642 (-) 564 WP_002469112.1 UvrB/UvrC motif-containing protein -
  MNU32_RS12265 (MNU32_12265) - 2525663..2526124 (-) 462 WP_001832288.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50303.88 Da        Isoelectric Point: 7.5278

>NTDB_id=662591 MNU32_RS12245 WP_002445730.1 2519751..2521124(-) (radA) [Staphylococcus epidermidis strain TSM-51]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKVAHPKHGVKTKETAGKVQKLNSVKHEATPRLLTESKEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICAALSQNKNVLYITGEESINQTKLRADRLEEDSSHLNVLAETDLEVIHQTVK
EERPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVLNPSEMFLEERSTNVPGSTIVPTMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSIIVATASSFKDQAVDGLDCFVGEVGLTGEVR
RVSRIEQRVQEATKLGFKRAIIPQTNIGGWTFPEGIQVVGVSSVHEALKYALHSKQR

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=662591 MNU32_RS12245 WP_002445730.1 2519751..2521124(-) (radA) [Staphylococcus epidermidis strain TSM-51]
ATGGCCAAGAAAAAAGTAATATTCGAATGTATGGCATGTGGCTATCAATCTCCTAAGTGGATGGGCAAGTGCCCTAACTG
CGGTGCGTGGAATCAAATGGAAGAAATAGTAGAAAAGGTTGCTCATCCTAAACATGGTGTAAAAACAAAAGAAACAGCAG
GTAAGGTACAAAAGTTAAATAGTGTTAAGCATGAAGCTACCCCTAGATTACTTACAGAATCAAAGGAATTTAACCGTGTT
TTAGGTGGAGGTATTGTAAGTGGTTCGTTAGTACTAATAGGTGGAGACCCAGGTATTGGTAAGTCGACTTTATTGCTACA
AATTTGTGCTGCGCTGTCACAAAATAAGAATGTTTTATATATTACTGGTGAGGAATCTATTAATCAAACTAAGTTGCGCG
CTGATCGTTTAGAAGAAGATTCTAGTCATCTTAATGTTTTAGCTGAAACTGATTTAGAAGTGATACATCAAACTGTAAAA
GAAGAGAGACCTGACTTACTTGTTGTTGATTCGATTCAAACAATCTATCATCCGGAAATTAGTTCCGCACCTGGATCGGT
ATCACAAGTAAGAGAGAGTACGCAGAGTTTAATGAACATTGCTAAACAAATGAATATTGCCACATTTATTGTGGGACACG
TAACAAAAGAAGGACAAATCGCCGGACCAAGATTATTGGAACATATGGTTGATACAGTTCTTTATTTTGAAGGAGATGAG
CATCACGCATATCGTATCCTTAGAGCAGTAAAAAATAGATTTGGTTCTACAAATGAGATGGGGATTTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTACTTAATCCTTCTGAAATGTTTTTAGAAGAACGTTCTACAAATGTTCCGGGCTCTACAATCG
TCCCCACTATGGAAGGAACAAGACCACTACTCATTGAAGTCCAAGCGCTTGTTACACCAACAACATTTAATAATCCTAGA
CGAATGGCTACAGGTATAGATCATAATCGATTAAGTTTACTTATGGCGGTTCTAGAAAAAAAGGAAAACTATTTACTCCA
ACAACAAGATGCCTATATTAAAGTAGCAGGTGGCGTCAAATTAACAGAACCTGCTGTTGATTTAAGCATTATTGTTGCGA
CAGCTTCAAGTTTTAAAGATCAAGCTGTTGATGGATTAGATTGTTTTGTGGGTGAAGTTGGATTAACAGGTGAAGTACGC
AGAGTATCTCGCATAGAGCAACGTGTTCAAGAAGCGACCAAACTAGGGTTTAAAAGAGCTATTATTCCACAGACAAATAT
TGGAGGTTGGACATTCCCAGAAGGCATCCAAGTCGTTGGTGTTTCATCAGTACATGAAGCTTTGAAATATGCATTACATT
CAAAACAGCGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(71-218)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y7VQ23

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.013

100

0.663
  radA Streptococcus pneumoniae Rx1

60.579

98.249

0.595
  radA Streptococcus pneumoniae D39

60.579

98.249

0.595
  radA Streptococcus pneumoniae R6

60.579

98.249

0.595
  radA Streptococcus pneumoniae TIGR4

60.579

98.249

0.595
  radA Streptococcus mitis NCTC 12261

60.579

98.249

0.595
  radA Streptococcus mitis SK321

60.579

98.249

0.595