Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   MNU33_RS00270 Genome accession   NZ_CP093208
Coordinates   55153..56502 (-) Length   449 a.a.
NCBI ID   WP_000815652.1    Uniprot ID   Q8VUV4
Organism   Staphylococcus epidermidis strain TSM-47     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 46738..78403 55153..56502 within 0


Gene organization within MGE regions


Location: 46738..78403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNU33_RS00230 (MNU33_00230) mecA 46738..48747 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  MNU33_RS00235 (MNU33_00235) - 48844..49830 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  MNU33_RS00240 (MNU33_00240) - 49802..50062 (+) 261 WP_001549961.1 type I restriction enzyme endonuclease domain-containing protein -
  MNU33_RS00245 (MNU33_00245) - 50053..51660 (-) 1608 Protein_46 IS1182 family transposase -
  MNU33_RS00250 (MNU33_00250) - 51712..52218 (-) 507 WP_001092189.1 DUF1643 domain-containing protein -
  MNU33_RS00255 (MNU33_00255) - 52233..52544 (-) 312 WP_000700851.1 DUF960 family protein -
  MNU33_RS00260 (MNU33_00260) - 52631..52981 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  MNU33_RS00265 (MNU33_00265) ccrB 53503..55131 (-) 1629 WP_001186604.1 recombinase family protein Machinery gene
  MNU33_RS00270 (MNU33_00270) ccrA 55153..56502 (-) 1350 WP_000815652.1 recombinase family protein Machinery gene
  MNU33_RS00275 (MNU33_00275) - 56736..58523 (-) 1788 WP_000122621.1 DUF927 domain-containing protein -
  MNU33_RS00280 (MNU33_00280) - 58523..58813 (-) 291 WP_001036437.1 hypothetical protein -
  MNU33_RS00285 (MNU33_00285) - 58952..60001 (+) 1050 WP_000613248.1 hypothetical protein -
  MNU33_RS00290 (MNU33_00290) - 60674..62164 (+) 1491 WP_000554504.1 RNA-binding domain-containing protein -
  MNU33_RS00295 (MNU33_00295) cstB 62537..63855 (-) 1319 Protein_56 persulfide dioxygenase-sulfurtransferase CstB -
  MNU33_RS00300 (MNU33_00300) - 63911..64048 (+) 138 WP_001791883.1 hypothetical protein -
  MNU33_RS00305 (MNU33_00305) - 64045..64416 (+) 372 WP_001549968.1 HNH endonuclease -
  MNU33_RS00310 (MNU33_00310) - 64545..65165 (+) 621 WP_000673114.1 hypothetical protein -
  MNU33_RS00315 (MNU33_00315) - 65589..65732 (+) 144 WP_001631028.1 restriction endonuclease subunit R -
  MNU33_RS00320 (MNU33_00320) - 65725..66927 (+) 1203 WP_001631029.1 restriction endonuclease subunit S -
  MNU33_RS00325 (MNU33_00325) - 66972..67188 (-) 217 Protein_62 IS5/IS1182 family transposase -
  MNU33_RS00330 (MNU33_00330) - 67250..67747 (-) 498 WP_001631030.1 GNAT family N-acetyltransferase -
  MNU33_RS00335 (MNU33_00335) - 68419..68829 (-) 411 WP_001631031.1 DUF2871 domain-containing protein -
  MNU33_RS00340 (MNU33_00340) - 68992..69212 (-) 221 Protein_65 hypothetical protein -
  MNU33_RS00345 (MNU33_00345) - 69227..69730 (-) 504 WP_001631032.1 DUF1643 domain-containing protein -
  MNU33_RS00350 (MNU33_00350) - 69746..70057 (-) 312 WP_001631033.1 DUF960 domain-containing protein -
  MNU33_RS00355 (MNU33_00355) - 70144..70494 (-) 351 WP_001631034.1 SAUGI family uracil-DNA glycosylase inhibitor -
  MNU33_RS00360 (MNU33_00360) ccrB 70995..72623 (-) 1629 WP_001631035.1 recombinase family protein Machinery gene
  MNU33_RS00365 (MNU33_00365) ccrA 72620..73981 (-) 1362 WP_001631036.1 recombinase family protein Machinery gene
  MNU33_RS00370 (MNU33_00370) - 74168..74797 (-) 630 Protein_71 hypothetical protein -
  MNU33_RS00375 (MNU33_00375) - 74806..75144 (+) 339 Protein_72 recombinase family protein -
  MNU33_RS00380 (MNU33_00380) - 75256..75615 (-) 360 WP_001631039.1 hypothetical protein -
  MNU33_RS00385 (MNU33_00385) - 75822..76148 (+) 327 WP_000391495.1 metalloregulator ArsR/SmtB family transcription factor -
  MNU33_RS00390 (MNU33_00390) - 76478..78403 (+) 1926 WP_002485723.1 heavy metal translocating P-type ATPase -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52616.30 Da        Isoelectric Point: 10.0062

>NTDB_id=662486 MNU33_RS00270 WP_000815652.1 55153..56502(-) (ccrA) [Staphylococcus epidermidis strain TSM-47]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYRVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYDYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRLQKQTKQTSSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKGAIDKRIKRQRNIEKHHILTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHTIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQAMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=662486 MNU33_RS00270 WP_000815652.1 55153..56502(-) (ccrA) [Staphylococcus epidermidis strain TSM-47]
ATGAAACAAGTTATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATGACACAATTAATTCAACAAGGACAATGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
TTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATATCGTGTTCATATCTTAAGTGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGATTATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGTCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACATCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAGGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACATATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATAAATGGGCATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAGCCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(169-248)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VUV4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

97.55

100

0.976
  ccrA Staphylococcus aureus COL

74.833

100

0.748