Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   MNU55_RS00300 Genome accession   NZ_CP093196
Coordinates   64320..65666 (-) Length   448 a.a.
NCBI ID   WP_000816499.1    Uniprot ID   Q9R3W9
Organism   Staphylococcus epidermidis strain TMDU-2014-62     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 42811..106184 64320..65666 within 0


Gene organization within MGE regions


Location: 42811..106184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNU55_RS00185 (MNU55_00185) mecA 42811..44820 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  MNU55_RS00190 (MNU55_00190) mecR1 44917..46674 (+) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  MNU55_RS00195 (MNU55_00195) mecI 46674..47045 (+) 372 WP_000369214.1 mecA-type methicillin resistance repressor MecI -
  MNU55_RS00200 (MNU55_00200) psm-mec 47130..47198 (-) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  MNU55_RS00205 (MNU55_00205) - 47532..48662 (+) 1131 WP_010922808.1 ROK family transcriptional regulator -
  MNU55_RS00210 (MNU55_00210) cstB 48776..50110 (-) 1335 Protein_39 persulfide dioxygenase-sulfurtransferase CstB -
  MNU55_RS00215 (MNU55_00215) - 50143..51207 (-) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  MNU55_RS00220 (MNU55_00220) cstR 51343..51603 (+) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  MNU55_RS00225 (MNU55_00225) - 51603..52241 (+) 639 Protein_42 sulfite exporter TauE/SafE family protein -
  MNU55_RS00230 (MNU55_00235) - 52515..53131 (-) 617 Protein_43 CadD family cadmium resistance transporter -
  MNU55_RS00235 (MNU55_00240) - 53212..55626 (-) 2415 WP_000378485.1 cadmium-translocating P-type ATPase CadA -
  MNU55_RS00240 (MNU55_00245) - 55619..55984 (-) 366 WP_000159127.1 metalloregulator ArsR/SmtB family transcription factor -
  MNU55_RS00245 (MNU55_00250) - 56364..56810 (-) 447 WP_000199401.1 GNAT family N-acetyltransferase -
  MNU55_RS00250 (MNU55_00255) - 56822..57241 (-) 420 WP_000616116.1 fosfomycin resistance bacillithiol transferase FosD -
  MNU55_RS00255 (MNU55_00260) - 57529..58122 (+) 594 WP_000690638.1 recombinase family protein -
  MNU55_RS00260 (MNU55_00265) - 58094..59536 (+) 1443 WP_002451367.1 Mu transposase C-terminal domain-containing protein -
  MNU55_RS00265 (MNU55_00270) - 59529..60344 (+) 816 WP_000164963.1 AAA family ATPase -
  MNU55_RS00270 (MNU55_00275) - 60408..60713 (-) 306 WP_254105957.1 JAB domain-containing protein -
  MNU55_RS00275 (MNU55_00280) - 60706..60912 (-) 207 WP_001077283.1 hypothetical protein -
  MNU55_RS00280 (MNU55_00285) - 60914..61435 (-) 522 WP_000836008.1 DUF1643 domain-containing protein -
  MNU55_RS00285 (MNU55_00290) - 61454..61765 (-) 312 WP_000833212.1 DUF960 family protein -
  MNU55_RS00290 (MNU55_00295) - 61850..62200 (-) 351 WP_000171449.1 SAUGI family uracil-DNA glycosylase inhibitor -
  MNU55_RS00295 (MNU55_00300) ccrB 62671..64299 (-) 1629 WP_001186596.1 recombinase family protein Machinery gene
  MNU55_RS00300 (MNU55_00305) ccrA 64320..65666 (-) 1347 WP_000816499.1 recombinase family protein Machinery gene
  MNU55_RS00305 (MNU55_00310) - 65859..66134 (-) 276 WP_001791132.1 hypothetical protein -
  MNU55_RS00310 (MNU55_00315) - 66224..68011 (-) 1788 WP_000181743.1 DUF927 domain-containing protein -
  MNU55_RS00315 (MNU55_00320) - 68011..68295 (-) 285 WP_000659554.1 DUF1413 domain-containing protein -
  MNU55_RS00320 (MNU55_00325) - 68433..69482 (+) 1050 WP_000613217.1 hypothetical protein -
  MNU55_RS00325 (MNU55_00330) - 69535..70107 (+) 573 WP_000489177.1 ADP-ribosyltransferase -
  MNU55_RS00330 (MNU55_00335) - 70439..70765 (+) 327 WP_000624048.1 helix-turn-helix domain-containing protein -
  MNU55_RS00335 (MNU55_00340) - 70802..71635 (+) 834 WP_000694291.1 CPBP family intramembrane glutamic endopeptidase -
  MNU55_RS00340 (MNU55_00345) - 71840..72004 (+) 165 WP_001028954.1 hypothetical protein -
  MNU55_RS00345 (MNU55_00350) - 72321..73094 (+) 774 WP_000998885.1 hypothetical protein -
  MNU55_RS00350 (MNU55_00355) - 73332..73691 (-) 360 WP_000432280.1 DUF4870 domain-containing protein -
  MNU55_RS00355 (MNU55_00360) - 73856..74926 (-) 1071 WP_001054882.1 nitronate monooxygenase family protein -
  MNU55_RS00360 (MNU55_00365) - 74939..75805 (-) 867 WP_001052247.1 LysR family transcriptional regulator -
  MNU55_RS00365 (MNU55_00370) - 75792..76982 (-) 1191 WP_000716034.1 YbfB/YjiJ family MFS transporter -
  MNU55_RS00370 (MNU55_00375) - 77193..77867 (+) 675 Protein_71 IS6 family transposase -
  MNU55_RS00375 (MNU55_00380) - 77927..78355 (-) 429 WP_000671997.1 VOC family protein -
  MNU55_RS00380 (MNU55_00385) - 78436..79365 (+) 930 WP_002504713.1 HTH domain-containing protein -
  MNU55_RS00385 (MNU55_00390) - 79527..81539 (+) 2013 WP_012088687.1 hypothetical protein -
  MNU55_RS00390 (MNU55_00395) - 81709..82818 (+) 1110 WP_254104810.1 DNA polymerase -
  MNU55_RS00395 (MNU55_00400) - 82811..83182 (+) 372 WP_002437511.1 hypothetical protein -
  MNU55_RS00400 (MNU55_00405) - 83179..84822 (+) 1644 WP_002504708.1 DUF5906 domain-containing protein -
  MNU55_RS00405 (MNU55_00410) - 85047..86723 (+) 1677 WP_002504707.1 recombinase family protein -
  MNU55_RS00410 (MNU55_00415) - 86829..87167 (+) 339 WP_000171557.1 SAUGI family uracil-DNA glycosylase inhibitor -
  MNU55_RS00415 (MNU55_00420) - 87263..87574 (+) 312 WP_001083108.1 DUF960 family protein -
  MNU55_RS00420 (MNU55_00425) - 87590..88096 (+) 507 WP_000849085.1 DUF1643 domain-containing protein -
  MNU55_RS00425 (MNU55_00430) - 88117..88434 (+) 318 WP_130825069.1 JAB domain-containing protein -
  MNU55_RS00430 (MNU55_00435) - 88553..89638 (+) 1086 WP_254105933.1 tyrosine-type recombinase/integrase -
  MNU55_RS00435 (MNU55_00440) - 89635..91527 (+) 1893 WP_254105940.1 site-specific integrase -
  MNU55_RS00440 (MNU55_00445) - 91534..91911 (+) 378 WP_000361059.1 DUF6262 family protein -
  MNU55_RS00445 (MNU55_00450) - 92062..92844 (+) 783 WP_000067268.1 aminoglycoside nucleotidyltransferase ANT(9)-Ia -
  MNU55_RS00450 (MNU55_00455) erm(A) 92970..93701 (-) 732 WP_254104725.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) -
  MNU55_RS12875 - 93784..93843 (-) 60 WP_010959190.1 erythromycin resistance leader peptide -
  MNU55_RS00455 (MNU55_00460) - 94235..94897 (+) 663 WP_001092058.1 class I SAM-dependent methyltransferase -
  MNU55_RS12820 - 95126..95275 (+) 150 Protein_90 JAB domain-containing protein -
  MNU55_RS00465 (MNU55_00470) - 95445..96317 (+) 873 WP_000373149.1 HTH domain-containing protein -
  MNU55_RS00470 (MNU55_00475) - 96374..96664 (+) 291 WP_000065261.1 hypothetical protein -
  MNU55_RS00475 (MNU55_00480) - 96680..97168 (+) 489 WP_000393586.1 hypothetical protein -
  MNU55_RS00480 (MNU55_00485) - 97303..97830 (+) 528 WP_002504704.1 hypothetical protein -
  MNU55_RS00485 (MNU55_00490) - 97892..98335 (+) 444 WP_000941333.1 hypothetical protein -
  MNU55_RS00490 (MNU55_00495) - 98284..98505 (+) 222 WP_002504703.1 hypothetical protein -
  MNU55_RS00495 (MNU55_00500) - 98899..99222 (+) 324 WP_001176049.1 PepSY domain-containing protein -
  MNU55_RS00500 (MNU55_00505) - 99300..102419 (-) 3120 WP_254105960.1 type I restriction endonuclease subunit R -
  MNU55_RS00505 (MNU55_00510) - 102403..103677 (-) 1275 WP_002504701.1 restriction endonuclease subunit S -
  MNU55_RS00510 (MNU55_00515) - 103667..105181 (-) 1515 WP_002504700.1 class I SAM-dependent DNA methyltransferase -
  MNU55_RS00515 (MNU55_00520) - 105372..106184 (+) 813 WP_002504699.1 abortive infection system antitoxin AbiGi family protein -

Sequence

Protein


Download         Length: 448 a.a.        Molecular weight: 52138.19 Da        Isoelectric Point: 10.1760

>NTDB_id=662345 MNU55_RS00300 WP_000816499.1 64320..65666(-) (ccrA) [Staphylococcus epidermidis strain TMDU-2014-62]
MKRAIGYLRQSTTKQQSLPAQKQAIELLAPKHNIQNIQYISDKQSGRTDNRTGYQQVTERIQQRQCDVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSVHDGYFDMDKAFDRLKLNIFMSLAELESDNIGEQVKNGLREKAKQGKLITTHAPFGYHYQ
NGTFIINNDESPTVKAVFNYYLQGYGYKKIAQYLEDDNKLITRKPYQVRNIIMNPNYCGRVINQYGQYNNMVPPIVSATK
YEHAQAIRNKKQLHCIPSENQLKQKIKCPCCDSTLTNMTIRKKHTLRYYICPKNMNESRFVCSFKGINAQKLEVQVLATC
QNFFQNQQLYSKINNAIHQRLKKQRVIEAKSTLTQEQLIDKLAKGMIDAESFRKQTHLMNQKHKTISSISDNQLQTSLQK
VIQKSFTLNMLHPYIDEIRITKNKALVGIYFKNEPLNIVNQTSQSSIA

Nucleotide


Download         Length: 1347 bp        

>NTDB_id=662345 MNU55_RS00300 WP_000816499.1 64320..65666(-) (ccrA) [Staphylococcus epidermidis strain TMDU-2014-62]
ATGAAACGAGCCATTGGTTATTTGCGCCAAAGTACAACGAAACAACAATCACTCCCAGCTCAAAAGCAAGCAATAGAATT
ATTAGCTCCAAAGCACAATATTCAAAATATCCAATACATTAGTGATAAGCAATCAGGCAGAACAGATAATCGAACAGGCT
ATCAACAAGTCACCGAACGCATCCAACAAAGACAATGTGACGTATTATGTTGTTATCGCTTGAATCGACTTCATCGCAAC
TTGAAAAATGCATTAAAACTCATGAAACTCTGTCAAAAATATCATGTTCATATTCTAAGTGTTCATGATGGCTATTTTGA
TATGGATAAAGCGTTTGATCGCCTAAAACTCAATATATTCATGAGTCTGGCTGAACTTGAATCCGATAATATTGGAGAAC
AAGTCAAAAATGGACTTAGAGAAAAGGCAAAACAAGGTAAACTCATAACGACCCATGCGCCTTTCGGTTATCACTATCAA
AATGGTACTTTCATCATTAATAATGATGAATCACCTACCGTCAAAGCTGTATTCAATTATTATCTTCAAGGATATGGCTA
CAAGAAGATTGCACAATATTTAGAAGACGATAATAAACTTATTACCCGCAAGCCTTATCAGGTACGAAATATAATTATGA
ACCCAAATTATTGTGGTCGTGTCATCAATCAATATGGTCAATATAACAATATGGTACCACCTATTGTTTCGGCAACGAAA
TATGAACATGCTCAAGCAATCCGTAATAAGAAGCAACTTCACTGTATACCTTCAGAGAATCAGCTGAAACAAAAGATCAA
ATGTCCTTGTTGTGACTCAACACTGACAAATATGACAATAAGAAAAAAACATACATTGCGATATTATATTTGTCCTAAAA
ATATGAATGAATCTCGCTTTGTCTGTTCATTCAAAGGAATAAATGCACAAAAATTAGAAGTTCAAGTCTTAGCTACATGT
CAGAACTTCTTTCAAAACCAACAGCTCTATTCAAAAATTAATAATGCAATTCATCAACGCCTCAAAAAACAAAGAGTGAT
AGAAGCTAAAAGTACGCTAACTCAAGAACAACTGATAGATAAACTTGCCAAAGGTATGATTGATGCTGAATCATTCAGAA
AACAGACTCATTTGATGAATCAAAAGCACAAAACCATATCCTCCATAAGTGATAATCAGTTACAAACATCACTACAAAAG
GTTATACAGAAAAGTTTCACGTTAAACATGCTGCATCCCTATATTGATGAAATTCGCATTACAAAAAATAAAGCCCTTGT
TGGGATCTATTTCAAAAATGAACCATTGAACATTGTGAACCAAACCTCGCAATCATCGATTGCTTAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(168-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9R3W9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

78.174

100

0.783
  ccrA Staphylococcus aureus N315

72.383

100

0.725