Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   MNU37_RS00285 Genome accession   NZ_CP093193
Coordinates   66407..67756 (-) Length   449 a.a.
NCBI ID   WP_020368079.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain TMDU-323     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 39729..67756 66407..67756 within 0


Gene organization within MGE regions


Location: 39729..67756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNU37_RS00160 (MNU37_00160) - 39729..41405 (+) 1677 WP_002504707.1 recombinase family protein -
  MNU37_RS00165 (MNU37_00165) - 41511..41849 (+) 339 WP_000171557.1 SAUGI family uracil-DNA glycosylase inhibitor -
  MNU37_RS00170 (MNU37_00170) - 41945..42256 (+) 312 WP_001083108.1 DUF960 family protein -
  MNU37_RS00175 (MNU37_00175) - 42272..42778 (+) 507 WP_000849085.1 DUF1643 domain-containing protein -
  MNU37_RS00180 (MNU37_00180) - 42799..43245 (+) 447 WP_002504705.1 RadC family protein -
  MNU37_RS00185 (MNU37_00185) - 43415..44287 (+) 873 WP_049374097.1 HTH domain-containing protein -
  MNU37_RS00190 (MNU37_00190) - 44339..44629 (+) 291 WP_049374096.1 hypothetical protein -
  MNU37_RS00195 (MNU37_00195) - 44645..45133 (+) 489 WP_000393586.1 hypothetical protein -
  MNU37_RS00200 (MNU37_00200) - 45268..45795 (+) 528 WP_000717928.1 hypothetical protein -
  MNU37_RS00205 (MNU37_00205) - 46458..49010 (-) 2553 WP_053032289.1 DEAD/DEAH box helicase family protein -
  MNU37_RS00210 (MNU37_00210) - 49013..51004 (-) 1992 WP_065428405.1 site-specific DNA-methyltransferase -
  MNU37_RS00215 (MNU37_00215) - 51143..52984 (-) 1842 WP_049374094.1 site-specific DNA-methyltransferase -
  MNU37_RS00220 (MNU37_00220) - 53564..54247 (+) 684 WP_049374093.1 hypothetical protein -
  MNU37_RS00225 (MNU37_00225) - 54704..55378 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  MNU37_RS00230 (MNU37_00230) - 55462..55803 (+) 342 WP_001797647.1 hydroxymethylglutaryl-CoA synthase -
  MNU37_RS00235 (MNU37_00235) - 56680..57423 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  MNU37_RS00240 (MNU37_00240) - 57520..57948 (+) 429 WP_000872606.1 MaoC family dehydratase -
  MNU37_RS00245 (MNU37_00245) mecA 57994..60003 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  MNU37_RS00250 (MNU37_00250) - 60100..61086 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  MNU37_RS00255 (MNU37_00255) - 61058..61318 (+) 261 WP_001549961.1 type I restriction enzyme endonuclease domain-containing protein -
  MNU37_RS00260 (MNU37_00260) - 61309..62916 (-) 1608 Protein_49 IS1182 family transposase -
  MNU37_RS00265 (MNU37_00265) - 62968..63474 (-) 507 WP_001092189.1 DUF1643 domain-containing protein -
  MNU37_RS00270 (MNU37_00270) - 63489..63800 (-) 312 WP_000700851.1 DUF960 family protein -
  MNU37_RS00275 (MNU37_00275) - 63887..64237 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  MNU37_RS00280 (MNU37_00280) ccrB 64757..66385 (-) 1629 WP_002495147.1 recombinase family protein Machinery gene
  MNU37_RS00285 (MNU37_00285) ccrA 66407..67756 (-) 1350 WP_020368079.1 recombinase family protein Machinery gene

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52505.24 Da        Isoelectric Point: 9.8772

>NTDB_id=662309 MNU37_RS00285 WP_020368079.1 66407..67756(-) (ccrA) [Staphylococcus epidermidis strain TMDU-323]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVKNGLQEKAKQGRLITTHAPFGYDYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDDTFINRQPYQVRNIIMNPNYCGRVINQYGQFDNMFPSIVSANV
YEQAQRLRSQKQTKQTPSDNQLKQKIKCPCCNATLTNMTVRKKNHILRYYVCPKNLNASRFVCDFKGINAQTLEDKVLKV
CQDFYQDHHIYAKIKNAIDKRLKRQRNTETHHTLTQEKLIEKLAQGIIDAETFRKQMQSLHQQPQRITSISEHQIQHTIQ
NIIQKRFTLNILYPYIETIHIAKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=662309 MNU37_RS00285 WP_020368079.1 66407..67756(-) (ccrA) [Staphylococcus epidermidis strain TMDU-323]
ATGAAACAAGTTATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATAACACAATTAATTCAACAAGGACAATGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACTTACCATGTTCATATCTTAAGCGTACATGATGGTTATTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCAGATAATATTGGAGAAC
AAGTCAAAAATGGCCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACACATGCACCTTTTGGCTACGACTATCAC
AACGGAACATTCATCATCAATCAAAATGAATCACCAACGGTGAAGGCTGTATTCAATTATTACATTAAAGGCCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATGATACGTTCATCAATCGACAACCTTATCAAGTTCGTAATATTATCATGA
ACCCTAATTATTGTGGTCGTGTTATCAACCAATACGGTCAATTCGACAATATGTTCCCTTCTATTGTTTCAGCAAATGTA
TATGAACAAGCGCAGAGACTTCGATCGCAAAAACAAACCAAACAGACACCTTCAGATAATCAACTAAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCGTTAGAAAAAAGAATCATATATTACGTTACTACGTCTGTCCTA
AAAACTTGAATGCTTCACGCTTTGTCTGTGATTTCAAAGGCATCAATGCACAGACATTGGAAGATAAAGTATTAAAAGTG
TGCCAAGACTTTTATCAAGACCACCATATCTACGCAAAAATTAAAAATGCGATTGACAAACGCTTAAAAAGACAACGAAA
CACAGAAACACATCACACACTGACTCAAGAAAAACTAATAGAAAAGTTAGCCCAAGGCATCATCGATGCAGAAACGTTCA
GAAAACAAATGCAATCATTACATCAACAACCGCAACGCATCACATCCATCAGTGAGCATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCTTATATTGAAACCATTCACATTGCGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCTCTCAATATCGTCAATCAAACCATGCAATCATCAATTGCATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase (Recombinase)

(169-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

92.205

100

0.922
  ccrA Staphylococcus aureus COL

75.278

100

0.753