Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MNU53_RS10750 Genome accession   NZ_CP093185
Coordinates   2275166..2276539 (-) Length   457 a.a.
NCBI ID   WP_001833080.1    Uniprot ID   A0AAE5QZG5
Organism   Staphylococcus epidermidis strain TMDU-300     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2270166..2281539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNU53_RS10735 (MNU53_10720) cysE 2271176..2271805 (-) 630 WP_002438669.1 serine O-acetyltransferase -
  MNU53_RS10740 (MNU53_10725) gltX 2272190..2273644 (-) 1455 WP_001832310.1 glutamate--tRNA ligase -
  MNU53_RS10745 (MNU53_10730) - 2274054..2275112 (-) 1059 WP_001832313.1 PIN/TRAM domain-containing protein -
  MNU53_RS10750 (MNU53_10735) radA 2275166..2276539 (-) 1374 WP_001833080.1 DNA repair protein RadA Machinery gene
  MNU53_RS10755 (MNU53_10740) - 2277030..2279483 (-) 2454 WP_001832300.1 ATP-dependent Clp protease ATP-binding subunit -
  MNU53_RS10760 (MNU53_10745) - 2279497..2280504 (-) 1008 WP_077755964.1 protein arginine kinase -
  MNU53_RS10765 (MNU53_10750) - 2280494..2281057 (-) 564 WP_001832265.1 UvrB/UvrC motif-containing protein -
  MNU53_RS10770 (MNU53_10755) - 2281078..2281539 (-) 462 WP_001832288.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50258.88 Da        Isoelectric Point: 7.5278

>NTDB_id=662267 MNU53_RS10750 WP_001833080.1 2275166..2276539(-) (radA) [Staphylococcus epidermidis strain TMDU-300]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKVAHPKHGVKTKETAGKVQKLNSVKHEATPRLLTESKEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICAALSQNKNVLYITGEESINQTKLRADRLEEDSSHLNVLAETDLEVIHQTVK
EERPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVLNPSEMFLEERSTNVPGSTIVPTMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSIIVATASSFKDQAVDGLDCFVGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRAIIPQTNIGGWTFPEGIQVVGVSSVHEALKYALHSKLR

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=662267 MNU53_RS10750 WP_001833080.1 2275166..2276539(-) (radA) [Staphylococcus epidermidis strain TMDU-300]
ATGGCCAAGAAAAAAGTAATATTCGAATGTATGGCATGTGGCTATCAATCTCCTAAGTGGATGGGCAAGTGCCCTAACTG
TGGTGCGTGGAATCAAATGGAAGAAATAGTAGAAAAGGTTGCTCATCCTAAACATGGTGTAAAAACAAAAGAAACAGCAG
GTAAGGTACAAAAGTTAAATAGCGTTAAGCATGAAGCTACCCCTAGATTACTTACAGAATCAAAGGAATTTAACCGTGTT
TTAGGTGGAGGTATTGTAAGTGGTTCGTTAGTACTAATAGGTGGAGACCCAGGTATTGGTAAGTCAACTTTATTGCTACA
AATTTGTGCTGCGCTGTCACAAAATAAGAATGTTTTATATATTACTGGTGAGGAATCTATTAATCAAACTAAGTTGCGCG
CTGATCGTTTGGAAGAAGATTCTAGTCATCTTAATGTTTTAGCCGAAACTGATTTAGAAGTGATACATCAAACTGTAAAA
GAAGAGAGACCTGACTTACTTGTTGTTGATTCGATTCAAACAATCTATCATCCGGAAATTAGTTCCGCACCTGGATCGGT
ATCACAAGTAAGAGAGAGTACGCAAAGTTTAATGAACATTGCTAAACAAATGAATATTGCCACATTTATTGTGGGACACG
TAACAAAAGAAGGACAAATCGCCGGACCAAGATTATTGGAACATATGGTTGATACAGTTCTTTATTTTGAAGGAGATGAG
CATCACGCATATCGTATCCTTAGAGCAGTAAAAAATAGATTTGGTTCTACAAATGAGATGGGGATTTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTACTTAATCCTTCTGAAATGTTTTTAGAAGAACGTTCTACAAATGTTCCGGGCTCTACAATCG
TCCCCACTATGGAAGGAACAAGACCACTACTCATTGAAGTCCAAGCGCTTGTTACACCAACAACATTTAATAATCCTAGA
CGAATGGCTACAGGTATAGATCATAATCGATTAAGTTTACTTATGGCGGTTCTAGAAAAAAAGGAAAACTATTTACTCCA
ACAACAAGATGCCTATATTAAAGTAGCAGGTGGCGTCAAATTAACAGAACCTGCTGTTGATTTAAGCATTATTGTTGCGA
CAGCTTCAAGTTTTAAAGATCAAGCTGTTGATGGATTAGATTGTTTTGTGGGTGAAGTTGGATTAACAGGTGAAGTACGC
AGAGTATCACGCATAGAGCAACGTGTTCAAGAAGCGGCCAAACTAGGGTTTAAAAGAGCTATTATTCCACAGACAAATAT
TGGAGGTTGGACATTCCCAGAAGGCATCCAAGTCGTTGGTGTTTCATCAGTACATGAAGCTTTGAAATATGCATTACATT
CAAAACTGCGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(71-218)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.009

99.781

0.659
  radA Streptococcus pneumoniae Rx1

60.802

98.249

0.597
  radA Streptococcus pneumoniae D39

60.802

98.249

0.597
  radA Streptococcus pneumoniae R6

60.802

98.249

0.597
  radA Streptococcus pneumoniae TIGR4

60.802

98.249

0.597
  radA Streptococcus mitis NCTC 12261

60.802

98.249

0.597
  radA Streptococcus mitis SK321

60.802

98.249

0.597