Detailed information NTG0066226

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NCGM1900_RS02040
• Genome accession: NZ_AP014622
• Coordinates: 438700..439848 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain NCGM1900
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 433700..444848

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NCGM1900_RS02015	-	434851..435147 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
NCGM1900_RS02020 (NCGM1900_0399)	-	435144..435737 (-)	594	WP_003084544.1	YggT family protein	-
NCGM1900_RS02025 (NCGM1900_0400)	proC	435748..436569 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
NCGM1900_RS02030 (NCGM1900_0401)	-	436581..437273 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
NCGM1900_RS02035 (NCGM1900_0402)	pilT	437488..438522 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
NCGM1900_RS02040 (NCGM1900_0403)	pilU	438700..439848 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
NCGM1900_RS02045 (NCGM1900_0404)	czcD	439856..440755 (-)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
NCGM1900_RS02050 (NCGM1900_0405)	-	440910..441314 (+)	405	WP_003084560.1	NINE protein	-
NCGM1900_RS02055 (NCGM1900_0406)	-	441557..442930 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
NCGM1900_RS02060 (NCGM1900_0407)	-	442927..444111 (+)	1185	WP_003084564.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=66226 NCGM1900_RS02040 WP_003100959.1 438700..439848(+) (pilU) [Pseudomonas aeruginosa strain NCGM1900]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=66226 NCGM1900_RS02040 WP_003100959.1 438700..439848(+) (pilU) [Pseudomonas aeruginosa strain NCGM1900]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382