Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   MNU53_RS00200 Genome accession   NZ_CP093185
Coordinates   45276..46625 (-) Length   449 a.a.
NCBI ID   WP_176430037.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain TMDU-300     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 43627..99998 45276..46625 within 0


Gene organization within MGE regions


Location: 43627..99998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNU53_RS00195 (MNU53_00195) - 43627..45254 (-) 1628 Protein_36 recombinase family protein -
  MNU53_RS00200 (MNU53_00200) ccrA 45276..46625 (-) 1350 WP_176430037.1 recombinase family protein Machinery gene
  MNU53_RS00205 (MNU53_00205) - 46859..48646 (-) 1788 WP_031880882.1 DUF927 domain-containing protein -
  MNU53_RS00210 (MNU53_00210) - 48766..50196 (-) 1431 WP_031880883.1 DUF6038 family protein -
  MNU53_RS00215 (MNU53_00215) - 50737..51786 (+) 1050 WP_031880884.1 ATP-binding protein -
  MNU53_RS00220 (MNU53_00220) - 51779..53992 (+) 2214 WP_031880885.1 S8 family peptidase -
  MNU53_RS00225 (MNU53_00225) - 54317..56332 (+) 2016 WP_002487819.1 hypothetical protein -
  MNU53_RS00230 (MNU53_00230) - 56357..57175 (-) 819 WP_002487821.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  MNU53_RS00235 (MNU53_00235) - 57172..57978 (-) 807 WP_002487840.1 site-specific DNA-methyltransferase -
  MNU53_RS00240 (MNU53_00240) - 58097..58318 (-) 222 WP_002487836.1 hypothetical protein -
  MNU53_RS00245 (MNU53_00245) - 58333..58836 (-) 504 WP_032604766.1 DUF1643 domain-containing protein -
  MNU53_RS00250 (MNU53_00250) - 58851..59162 (-) 312 WP_002487841.1 DUF960 family protein -
  MNU53_RS00255 (MNU53_00255) - 59249..59599 (-) 351 WP_002487822.1 SAUGI family uracil-DNA glycosylase inhibitor -
  MNU53_RS00260 (MNU53_00260) - 60139..60756 (-) 618 WP_002487818.1 hypothetical protein -
  MNU53_RS00265 (MNU53_00265) ccrB 60883..62508 (-) 1626 WP_254104479.1 recombinase family protein Machinery gene
  MNU53_RS00270 (MNU53_00270) ccrA 62505..63866 (-) 1362 WP_002487826.1 recombinase family protein Machinery gene
  MNU53_RS00275 (MNU53_00275) - 64053..65822 (-) 1770 WP_002487833.1 DUF927 domain-containing protein -
  MNU53_RS00280 (MNU53_00280) - 65822..66112 (-) 291 WP_002487823.1 DUF1413 domain-containing protein -
  MNU53_RS00285 (MNU53_00285) - 66283..67353 (+) 1071 WP_002487837.1 hypothetical protein -
  MNU53_RS00290 (MNU53_00290) - 67447..69387 (+) 1941 WP_002487831.1 ATP-dependent DNA helicase -
  MNU53_RS00295 (MNU53_00295) - 69685..72219 (-) 2535 WP_252911808.1 heavy metal translocating P-type ATPase -
  MNU53_RS00300 (MNU53_00300) - 72228..72542 (-) 315 WP_002487824.1 helix-turn-helix transcriptional regulator -
  MNU53_RS00305 (MNU53_00305) - 72693..73940 (+) 1248 WP_002487828.1 MFS transporter -
  MNU53_RS00310 (MNU53_00310) - 74015..74659 (+) 645 WP_002487838.1 metalloregulator ArsR/SmtB family transcription factor -
  MNU53_RS00315 (MNU53_00315) - 75039..75365 (+) 327 WP_012085243.1 helix-turn-helix domain-containing protein -
  MNU53_RS00320 (MNU53_00320) - 75402..76220 (+) 819 WP_002484875.1 CPBP family intramembrane glutamic endopeptidase -
  MNU53_RS00325 (MNU53_00325) - 76321..76995 (-) 675 Protein_62 IS6 family transposase -
  MNU53_RS00330 (MNU53_00330) - 77271..78412 (+) 1142 WP_089418807.1 IS3 family transposase -
  MNU53_RS00335 (MNU53_00335) - 78481..79050 (-) 570 WP_002487775.1 TetR/AcrR family transcriptional regulator -
  MNU53_RS00340 (MNU53_00340) - 79400..80344 (-) 945 WP_002484621.1 glycine betaine ABC transporter substrate-binding protein -
  MNU53_RS00345 (MNU53_00345) - 80368..83049 (-) 2682 WP_002484615.1 MMPL family transporter -
  MNU53_RS00350 (MNU53_00350) - 83425..83778 (+) 354 WP_002487778.1 hypothetical protein -
  MNU53_RS00355 (MNU53_00355) - 84023..84877 (-) 855 WP_223297891.1 sensor histidine kinase KdpD -
  MNU53_RS00360 (MNU53_00360) - 84968..85723 (-) 756 WP_002487777.1 ABC transporter permease -
  MNU53_RS00365 (MNU53_00365) - 85716..86630 (-) 915 WP_002487779.1 ATP-binding cassette domain-containing protein -
  MNU53_RS00370 (MNU53_00370) - 86721..87389 (-) 669 WP_002487782.1 response regulator transcription factor -
  MNU53_RS00375 (MNU53_00375) - 87999..89417 (-) 1419 WP_012087795.1 aspartate aminotransferase family protein -
  MNU53_RS11875 - 89506..89718 (-) 213 WP_002498809.1 DUF1958 domain-containing protein -
  MNU53_RS00380 (MNU53_00380) - 90364..90567 (+) 204 WP_002487774.1 hypothetical protein -
  MNU53_RS00385 (MNU53_00385) - 90995..92251 (+) 1257 WP_000566670.1 DUF3578 domain-containing protein -
  MNU53_RS00390 (MNU53_00390) - 92554..93228 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  MNU53_RS00395 (MNU53_00395) - 93312..93653 (+) 342 WP_001797647.1 hydroxymethylglutaryl-CoA synthase -
  MNU53_RS00400 (MNU53_00400) - 94450..95193 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  MNU53_RS00405 (MNU53_00405) - 95290..95718 (+) 429 WP_000872606.1 MaoC family dehydratase -
  MNU53_RS00410 (MNU53_00410) mecA 95764..97773 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  MNU53_RS00415 (MNU53_00415) mecR1 97870..99627 (+) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  MNU53_RS00420 (MNU53_00420) mecI 99627..99998 (+) 372 WP_000369214.1 mecA-type methicillin resistance repressor MecI -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52605.32 Da        Isoelectric Point: 9.9534

>NTDB_id=662235 MNU53_RS00200 WP_176430037.1 45276..46625(-) (ccrA) [Staphylococcus epidermidis strain TMDU-300]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYRVHILSVHDGYFDMDQAFDRFKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGAFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRSQKQIKQTSSDNQLKQKIKCPCCNATLTNMTVRKKNHILRYYVCPKNLNASRFVCDFKGINAQTLEDKVLKV
CQDFYQDHHIYAKIKNAIDKRLKRQRNTETHHTLTQEKLIEKLAQGIIDAETFRKQMQSLHQQPQRITSISEHQIQHTIQ
NIIQKRFTLNILYPYIETIHIAKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=662235 MNU53_RS00200 WP_176430037.1 45276..46625(-) (ccrA) [Staphylococcus epidermidis strain TMDU-300]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATGACACAATTAATTCAACAAGGACAATGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
TTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACATATCGTGTTCATATCTTAAGTGTGCATGATGGTTATTTTGA
TATGGATCAAGCTTTCGACCGATTCAAGCTTAATATTTTTATCAGCTTGGCCGAACTTGAATCAGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACCCATGCGCCCTTTGGTTACGAATATCAC
AACGGAGCATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATCGCAAAAACAAATCAAACAGACATCTTCGGATAACCAACTAAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCGTTAGAAAAAAGAATCATATATTACGTTACTACGTCTGTCCTA
AAAACTTGAATGCTTCACGCTTTGTCTGTGATTTCAAAGGCATCAATGCACAGACATTGGAAGATAAAGTATTAAAAGTG
TGCCAAGACTTTTATCAAGACCACCATATCTACGCAAAAATTAAAAATGCGATTGACAAACGCTTAAAAAGACAACGAAA
CACAGAAACACATCACACACTGACTCAAGAAAAACTAATAGAAAAGTTAGCCCAAGGCATCATCGATGCAGAAACGTTCA
GAAAACAAATGCAATCATTACATCAACAACCGCAACGCATCACATCCATCAGTGAGCATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTGAACATATTGTACCCCTATATTGAAACCATTCACATTGCGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAACCATGCAATCATCAATTGCATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)

Recombinase (Recombinase)

(169-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

95.1

100

0.951
  ccrA Staphylococcus aureus COL

73.719

100

0.737