Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MND42_RS02165 Genome accession   NZ_CP093154
Coordinates   462690..464123 (-) Length   477 a.a.
NCBI ID   WP_045403547.1    Uniprot ID   -
Organism   Enterobacter asburiae strain 5549     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 457690..469123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MND42_RS02135 (MND42_02135) elbB 459222..459875 (+) 654 WP_045403552.1 isoprenoid biosynthesis glyoxalase ElbB -
  MND42_RS02140 (MND42_02140) mtgA 459872..460597 (+) 726 WP_045403550.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  MND42_RS02145 (MND42_02145) npr 460637..460909 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  MND42_RS02150 (MND42_02150) rapZ 460906..461760 (-) 855 WP_014885397.1 RNase adapter RapZ -
  MND42_RS02155 (MND42_02155) ptsN 461806..462297 (-) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  MND42_RS02160 (MND42_02160) hpf 462380..462667 (-) 288 WP_008501444.1 ribosome hibernation promoting factor -
  MND42_RS02165 (MND42_02165) rpoN/rpoN1 462690..464123 (-) 1434 WP_045403547.1 RNA polymerase factor sigma-54 Machinery gene
  MND42_RS02170 (MND42_02170) lptB 464171..464896 (-) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  MND42_RS02175 (MND42_02175) lptA 464903..465457 (-) 555 WP_023309358.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  MND42_RS02180 (MND42_02180) lptC 465426..466001 (-) 576 WP_023309357.1 LPS export ABC transporter periplasmic protein LptC -
  MND42_RS02185 (MND42_02185) kdsC 465998..466564 (-) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  MND42_RS02190 (MND42_02190) kdsD 466579..467565 (-) 987 WP_023309355.1 arabinose-5-phosphate isomerase KdsD -
  MND42_RS02195 (MND42_02195) - 467579..468556 (-) 978 WP_023309354.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54183.92 Da        Isoelectric Point: 4.3173

>NTDB_id=662002 MND42_RS02165 WP_045403547.1 462690..464123(-) (rpoN/rpoN1) [Enterobacter asburiae strain 5549]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWIDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=662002 MND42_RS02165 WP_045403547.1 462690..464123(-) (rpoN/rpoN1) [Enterobacter asburiae strain 5549]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
GCTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTACCAGGGTGAGACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGTGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATTCTGGAAAGCATGGGCGACGAAGAGATTGAGCTTGAGGAAGTCGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGATCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCCAACCACGATTTCCGCAGCCTGATGCGCGTCACGCGG
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAGTACGTCATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGTAT
TGTTGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.545

100

0.482