Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MM522_RS20745 Genome accession   NZ_CP093067
Coordinates   4014883..4015332 (-) Length   149 a.a.
NCBI ID   WP_003246436.1    Uniprot ID   A0AAE2SFQ0
Organism   Bacillus subtilis strain ATC-3     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4009883..4020332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MM522_RS20710 (MM522_20710) yckA 4010155..4010835 (-) 681 WP_024572176.1 amino acid ABC transporter permease -
  MM522_RS20715 (MM522_20715) yckB 4010845..4011708 (-) 864 WP_009966510.1 transporter substrate-binding domain-containing protein -
  MM522_RS20720 (MM522_20720) - 4011870..4012066 (+) 197 Protein_4028 hypothetical protein -
  MM522_RS20725 (MM522_20725) - 4012106..4012420 (+) 315 Protein_4029 RDD family protein -
  MM522_RS20730 (MM522_20730) yckD 4012502..4012834 (+) 333 WP_003246247.1 YckD family protein -
  MM522_RS20735 (MM522_20735) bglC 4012988..4014421 (+) 1434 WP_003246242.1 6-phospho-beta-glucosidase -
  MM522_RS20740 (MM522_20740) nin/comJ 4014458..4014856 (-) 399 WP_003246342.1 DNA-entry nuclease inhibitor Regulator
  MM522_RS20745 (MM522_20745) nucA/comI 4014883..4015332 (-) 450 WP_003246436.1 DNA-entry nuclease Machinery gene
  MM522_RS20750 (MM522_20750) tlpC 4015500..4017221 (-) 1722 WP_003246498.1 methyl-accepting chemotaxis protein TlpC -
  MM522_RS20755 (MM522_20755) hxlB 4017332..4017889 (-) 558 WP_003246343.1 6-phospho-3-hexuloisomerase -
  MM522_RS20760 (MM522_20760) hxlA 4017895..4018527 (-) 633 WP_003246452.1 3-hexulose-6-phosphate synthase -
  MM522_RS20765 (MM522_20765) hxlR 4018760..4019122 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16486.48 Da        Isoelectric Point: 4.6274

>NTDB_id=661886 MM522_RS20745 WP_003246436.1 4014883..4015332(-) (nucA/comI) [Bacillus subtilis strain ATC-3]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHRLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=661886 MM522_RS20745 WP_003246436.1 4014883..4015332(-) (nucA/comI) [Bacillus subtilis strain ATC-3]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATGGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
GGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

100

100

1