Detailed information NTG0661119

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: MME54_RS04605
• Genome accession: NZ_CP092918
• Coordinates: 1019484..1020851 (+)
• Length: 455 a.a.
• NCBI ID: WP_054984806.1
• Uniprot ID: -
• Organism: Pseudomonas syringae pv. helianthi strain LMG 5067
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1014484..1025851

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MME54_RS04575 (MME54_04575)	cydB	1014640..1015647 (+)	1008	WP_054984802.1	cytochrome d ubiquinol oxidase subunit II	-
MME54_RS04580 (MME54_04580)	-	1015669..1015836 (+)	168	WP_061447419.1	DUF2474 domain-containing protein	-
MME54_RS04585 (MME54_04585)	-	1015923..1017047 (-)	1125	WP_054984803.1	methyltransferase	-
MME54_RS04590 (MME54_04590)	-	1017179..1017412 (-)	234	WP_082426990.1	helix-turn-helix domain-containing protein	-
MME54_RS04595 (MME54_04595)	-	1017749..1018525 (+)	777	WP_054984804.1	ferredoxin--NADP reductase	-
MME54_RS04600 (MME54_04600)	mscL	1018842..1019288 (-)	447	WP_054984805.1	large-conductance mechanosensitive channel protein MscL	-
MME54_RS04605 (MME54_04605)	radA	1019484..1020851 (+)	1368	WP_054984806.1	DNA repair protein RadA	Machinery gene
MME54_RS04610 (MME54_04610)	-	1020858..1021226 (-)	369	WP_054984822.1	PilZ domain-containing protein	-
MME54_RS04615 (MME54_04615)	-	1021512..1023587 (+)	2076	WP_054984807.1	carbon starvation CstA family protein	-
MME54_RS04620 (MME54_04620)	-	1023615..1023812 (+)	198	WP_003313802.1	YbdD/YjiX family protein	-
MME54_RS04625 (MME54_04625)	yjiA	1023908..1024885 (+)	978	WP_054984808.1	GTPase	-
MME54_RS04630 (MME54_04630)	-	1024944..1025207 (-)	264	Protein_912	PAAR domain-containing protein	-

Sequence

Protein

• Length: 455 a.a.
• Molecular weight: 48869.43 Da
• Isoelectric Point: 7.1316

>NTDB_id=661119 MME54_RS04605 WP_054984806.1 1019484..1020851(+) (radA) [Pseudomonas syringae pv. helianthi strain LMG 5067]
MAKAKRLYGCTECGATFPKWAGQCGECGAWNTLVETMLESGAAAPPSGRTGWTGSQAQIRTLAEVSVEEIPRFSTNSTEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAQRMPALYVTGEESQQQVAMRARRLGLPQDKLRVMTETCIETIIA
TAKVEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHMS
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE

Nucleotide

• Length: 1368 bp

>NTDB_id=661119 MME54_RS04605 WP_054984806.1 1019484..1020851(+) (radA) [Pseudomonas syringae pv. helianthi strain LMG 5067]
ATGGCCAAGGCCAAACGCTTGTACGGCTGCACCGAGTGCGGCGCGACATTTCCCAAGTGGGCGGGCCAGTGCGGCGAATG
CGGTGCGTGGAACACACTGGTCGAAACCATGCTTGAAAGTGGTGCCGCCGCGCCACCCAGCGGGCGTACCGGCTGGACCG
GTTCTCAGGCGCAGATCAGGACTCTGGCGGAAGTCAGCGTTGAAGAAATTCCGCGCTTTTCCACCAACTCCACCGAACTG
GACAGAGTGCTGGGCGGTGGTCTGGTCGATGGTTCGGTGGTGTTGATCGGCGGCGACCCCGGCATCGGCAAATCCACGAT
CCTCTTGCAGACGCTGTGCAATATCGCCCAGCGCATGCCTGCGTTGTATGTCACCGGTGAGGAATCCCAGCAGCAGGTGG
CCATGCGCGCCCGTCGTCTGGGGCTGCCGCAGGACAAGCTGCGGGTCATGACCGAAACCTGCATCGAGACCATCATTGCC
ACGGCAAAGGTCGAGAAGCCCAAGGTCATGGTGATCGATTCGATCCAGACAATTTTCACCGAGCAATTGCAATCCGCACC
GGGCGGCGTGTCTCAGGTTCGTGAAAGCGCCGCCTTACTGGTGCGTTATGCCAAACAAAGCGGTACTGCGATCTTCCTGG
TTGGTCATGTCACCAAGGAAGGCGCCCTGGCCGGCCCGCGTGTGCTTGAGCACATGGTCGACACCGTATTGTATTTCGAG
GGCGAGTCGGATGGTCGCCTGCGGCTGCTGCGGGCGGTGAAGAACCGCTTTGGCGCGGTAAACGAGCTGGGCGTATTCGG
CATGACCGACAAGGGGCTTAAAGAAGTCTCCAACCCGTCGGCGATTTTTCTTACTCGTGCGCAGGAAGAAGTGCCGGGCA
GTGTGGTCATGGCTACCTGGGAAGGCACGCGGCCGATGCTGGTGGAGGTTCAGGCACTGGTCGACGATAGCCACATGTCC
AATCCGCGCCGTGTGACGCTGGGCCTGGATCAGAATCGCCTAGCCATGCTGCTGGCGGTGCTGCATCGACACGGCGGTAT
TCCGACCCATGATCAGGACGTGTTTCTCAACGTGGTGGGCGGTGTGAAAGTGCTGGAAACCGCATCCGATCTGGCCTTGA
TGGCGGCAGTGATGTCCAGCCTGCGCAATCGTCCGTTGCCGCACGACTTGCTGGTGTTTGGCGAAGTAGGGCTGTCCGGT
GAGGTCAGGCCGGTGCCCAGCGGGCAGGAGCGCCTCAAGGAGGCCGCCAAGCACGGCTTCAAACGTGCCATCGTGCCTAA
AGGCAACGCGCCGAAGGAAGCACCACCCGGCCTGCAGATCATCGCCGTCACGCGCCTTGAGCAGGCACTCGATGCGCTGT
TCGAGTAG

Domains

Predicted by InterproScan.

• AAA domain (AAA_25) (73-221)

• Subunit ChlI of Mg-chelatase (ChlI) (353-429)

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Bacillus subtilis subsp. subtilis str. 168	48.904	100	0.49
radA	Streptococcus pneumoniae Rx1	46.739	100	0.473
radA	Streptococcus pneumoniae R6	46.739	100	0.473
radA	Streptococcus pneumoniae TIGR4	46.739	100	0.473
radA	Streptococcus pneumoniae D39	46.739	100	0.473
radA	Streptococcus mitis SK321	46.93	100	0.47
radA	Streptococcus mitis NCTC 12261	46.711	100	0.468