Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M3B_RS01060 Genome accession   NZ_AP014596
Coordinates   190949..192310 (+) Length   453 a.a.
NCBI ID   WP_011054159.1    Uniprot ID   A0A5S4TL01
Organism   Streptococcus pyogenes strain M3-b     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 185949..197310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3B_RS01045 (M3B_0211) - 187643..189427 (+) 1785 WP_011054157.1 ABC transporter ATP-binding protein -
  M3B_RS01050 (M3B_0212) - 189545..190312 (+) 768 WP_011054158.1 epoxyqueuosine reductase QueH -
  M3B_RS01055 (M3B_0213) - 190422..190868 (+) 447 WP_002986111.1 dUTP diphosphatase -
  M3B_RS01060 (M3B_0214) radA 190949..192310 (+) 1362 WP_011054159.1 DNA repair protein RadA Machinery gene
  M3B_RS01065 (M3B_0215) - 192499..192996 (+) 498 WP_011054160.1 beta-class carbonic anhydrase -
  M3B_RS01070 (M3B_0216) - 193127..193837 (+) 711 WP_011017311.1 TIGR00266 family protein -
  M3B_RS01075 (M3B_0217) gltX 194020..195465 (+) 1446 WP_011054161.1 glutamate--tRNA ligase -
  M3B_RS01080 (M3B_0218) - 195860..197206 (+) 1347 WP_002991186.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49348.58 Da        Isoelectric Point: 6.0962

>NTDB_id=66082 M3B_RS01060 WP_011054159.1 190949..192310(+) (radA) [Streptococcus pyogenes strain M3-b]
MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKNARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRV
LGGGVVPGSLILIGGDPGIGKSTLLLQVSTQLANKGTVLYASGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKVYAPKNALQGIDISQGIEVVGVTTVGQVLKAVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=66082 M3B_RS01060 WP_011054159.1 190949..192310(+) (radA) [Streptococcus pyogenes strain M3-b]
ATGGCAAAGAAAAAAGCAACCTTTATCTGTCAGGAGTGTGGCTACCAGTCGCCAAAGTATTTAGGGCGCTGTCCAAATTG
CTCGGCCTGGTCTTCTTTTGTGGAAGAAGTAGAGGTCAAAGAAGTCAAAAATGCCCGTGTTAGTCTAGCTGGAGAAAAGT
CCAGACCGGTTAAACTAAAAGATGTGGATAATATTAGTTATCACAGAACACAGACTGACATGTCTGAATTTAATAGAGTT
CTTGGAGGGGGTGTGGTTCCTGGAAGCTTGATTTTAATTGGTGGTGATCCAGGTATTGGAAAATCCACCTTACTCTTGCA
GGTATCTACGCAGCTCGCTAATAAGGGAACGGTTCTCTATGCGTCTGGGGAAGAATCAGCAGAGCAGATTAAACTGCGGA
GTGAGCGTCTGGGTGATATTGACAATGAATTTTACCTCTATGCCGAAACCAATATGCAAGCTATTCGCACAGAAATTGAA
AATATTAAACCAGATTTTTTAATTATTGACTCCATTCAGACCATCATGAGCCCTGATATTACAGGTGTTCAAGGATCAGT
CAGTCAGGTGAGAGAAGTCACTGCAGAATTGATGCAGCTTGCAAAGACGAACAACATTGCAACCTTTATTGTGGGTCATG
TCACCAAGGAAGGAACCTTGGCTGGGCCTCGTATGTTAGAACACATGGTGGATACCGTTCTCTACTTTGAAGGGGAAAGG
CATCACACCTTTCGCATTCTTCGGGCTGTGAAAAACCGCTTTGGCTCCACTAACGAGATTGGCATCTTTGAAATGCAGTC
TGGCGGCTTGGTAGAGGTCTTAAATCCTAGTCAAGTCTTTTTAGAGGAACGCTTAGATGGGGCAACAGGATCGGCTGTCG
TTGTAACGATGGAAGGAAGTCGTCCTATCTTAGCAGAGGTGCAATCTTTAGTGACACCAACCGTTTTTGGCAATGCCAGA
CGAACCACTACGGGACTTGATTTCAATCGCGTAAGCCTAATAATGGCAGTTTTAGAAAAGCGTTGTGGTTTACTCTTACA
AAATCAAGATGCCTACCTCAAGTCGGCTGGTGGTGTCAAACTAGACGAACCTGCCATTGATTTGGCAGTCGCTGTGGCTA
TCGCATCCAGTTATAAGGAAAAACCAACCAGCCCTCAAGAAGCTTTCTTAGGCGAGATTGGGTTAACAGGTGAGATTCGG
CGAGTGACCCGTATTGAGCAACGGATTAATGAAGCAGCAAAGCTTGGCTTTACCAAGGTCTATGCTCCTAAAAATGCCTT
GCAAGGCATTGATATCTCTCAAGGTATTGAGGTTGTTGGAGTGACTACGGTTGGTCAGGTTTTAAAGGCAGTTTTTAGCT
AG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(56-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4TL01

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

85.43

100

0.854
  radA Streptococcus pneumoniae Rx1

85.21

100

0.852
  radA Streptococcus pneumoniae D39

85.21

100

0.852
  radA Streptococcus pneumoniae R6

85.21

100

0.852
  radA Streptococcus pneumoniae TIGR4

85.21

100

0.852
  radA Streptococcus mitis SK321

85.21

100

0.852
  radA Bacillus subtilis subsp. subtilis str. 168

61.148

100

0.611

Multiple sequence alignment