Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MK974_RS14090 Genome accession   NZ_CP092843
Coordinates   3069537..3071042 (+) Length   501 a.a.
NCBI ID   WP_105832999.1    Uniprot ID   -
Organism   Burkholderia ambifaria strain Q53     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3064537..3076042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK974_RS14065 (MK974_14055) kdsD 3065770..3066753 (+) 984 WP_126228526.1 arabinose 5-phosphate isomerase KdsD -
  MK974_RS14070 (MK974_14060) - 3066753..3067289 (+) 537 WP_105833002.1 HAD family hydrolase -
  MK974_RS14075 (MK974_14065) lptC 3067293..3067907 (+) 615 WP_126228527.1 LPS export ABC transporter periplasmic protein LptC -
  MK974_RS14080 (MK974_14070) lptA 3067928..3068593 (+) 666 WP_011657963.1 lipopolysaccharide transport periplasmic protein LptA -
  MK974_RS14085 (MK974_14075) lptB 3068590..3069366 (+) 777 WP_011657964.1 LPS export ABC transporter ATP-binding protein -
  MK974_RS14090 (MK974_14080) rpoN/rpoN1 3069537..3071042 (+) 1506 WP_105832999.1 RNA polymerase factor sigma-54 Machinery gene
  MK974_RS14095 (MK974_14085) raiA 3071200..3071559 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  MK974_RS14100 (MK974_14090) - 3071729..3072268 (+) 540 WP_011657966.1 PTS sugar transporter subunit IIA -
  MK974_RS14105 (MK974_14095) hprK 3072398..3073366 (+) 969 WP_011657967.1 HPr(Ser) kinase/phosphatase -
  MK974_RS14110 (MK974_14100) rapZ 3073442..3074350 (+) 909 WP_041489859.1 RNase adapter RapZ -
  MK974_RS14115 (MK974_14105) - 3074377..3075012 (+) 636 WP_006752630.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54712.79 Da        Isoelectric Point: 4.8796

>NTDB_id=660761 MK974_RS14090 WP_105832999.1 3069537..3071042(+) (rpoN/rpoN1) [Burkholderia ambifaria strain Q53]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENEDEWITSPLRVASDGSLIAQGPSGQNIEPGP
ANGSSPSSERAERDEPAGADEYDGYGSGDAGDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLSDLPPELEVDGDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=660761 MK974_RS14090 WP_105832999.1 3069537..3071042(+) (rpoN/rpoN1) [Burkholderia ambifaria strain Q53]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACCCCGCAACTGCAGCAGTCGATCCGGCTCCTGCA
ACTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAAAATCCGTTGCTCGAAAACGAAGACGAATGGA
TCACGAGTCCGCTGCGTGTCGCGTCGGACGGATCGCTGATCGCGCAGGGACCTTCCGGACAGAACATCGAGCCGGGCCCC
GCGAACGGCAGCAGCCCGTCGAGCGAGCGCGCCGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGGCTACGGGTC
GGGCGACGCCGGCGACGGCCCGCAGTGGAATCTGGACGAGTACGGCCGCTCGAGCGGCGCATCGGACGATGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAATTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCACTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCATCGCTCGACGAAGTCCT
CTCCGACCTGCCGCCGGAACTGGAAGTCGACGGCGACGAACTGAATGCGGCGCTCGCGCTGCTGCACAGTTTCGACCCGG
CCGGGGTCGGGGCCCGCTCGGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCCACCCGCACGCTC
GCGCTGGAGATCGTGTCGCAGCACCTCGAGCTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTATGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAACTCAATCCGGAAGTGATGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGGTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCCATTGTCG
AACGTCAAAAGAATTTCTTCGCGCATGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACAGGCAAGTACATGCTGACCCCGTTCGGTACGCTGGAATTCAAGTACTT
CTTCGGGTCACACGTTTCGACCGACACCGGAGGCGCCGCATCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTTGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAAATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.515

100

0.691