Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MK974_RS10165 Genome accession   NZ_CP092843
Coordinates   2213196..2214572 (-) Length   458 a.a.
NCBI ID   WP_011657301.1    Uniprot ID   Q0BDZ7
Organism   Burkholderia ambifaria strain Q53     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2208196..2219572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK974_RS10145 (MK974_10150) cls 2208495..2209934 (+) 1440 WP_269015714.1 cardiolipin synthase -
  MK974_RS10150 (MK974_10155) - 2210055..2210534 (-) 480 WP_011657298.1 glutathione peroxidase -
  MK974_RS10155 (MK974_10160) - 2210671..2212602 (+) 1932 WP_269015715.1 ATP-binding cassette domain-containing protein -
  MK974_RS10160 (MK974_10165) - 2212783..2213049 (-) 267 WP_011657300.1 DUF2866 domain-containing protein -
  MK974_RS10165 (MK974_10170) radA 2213196..2214572 (-) 1377 WP_011657301.1 DNA repair protein RadA Machinery gene
  MK974_RS10170 (MK974_10175) alr 2214584..2215654 (-) 1071 WP_011657302.1 alanine racemase -
  MK974_RS10175 (MK974_10180) lplT 2215873..2217180 (+) 1308 WP_269015716.1 lysophospholipid transporter LplT -
  MK974_RS10180 (MK974_10185) thiD 2217239..2218048 (+) 810 WP_175668920.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  MK974_RS10185 (MK974_10190) - 2218067..2219026 (-) 960 WP_269015717.1 DUF1853 family protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 48820.25 Da        Isoelectric Point: 6.9501

>NTDB_id=660757 MK974_RS10165 WP_011657301.1 2213196..2214572(-) (radA) [Burkholderia ambifaria strain Q53]
MAKQKTVYVCSECGGQTPKWQGQCPSCQAWNTLVESVAESPAAHRFQSLAKRAPVQRLVDIEAADVPRFSTGIGEFDRVL
GGGLVPGGVVLIGGDPGIGKSTLLLQSLADIANERRALYISGEESAAQIALRAQRLALLDGGAPAAELKLLAEIQLEKIQ
AAIDAERPDVAVIDSIQTVYSEALTSAPGSVAQVRECAAQLTRIAKQSGTAIIMVGHVTKEGNLAGPRVLEHIVDTVLYF
EGDTHSSFRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHEQVVPGSCVLVTQEGTRPLLVEVQALVDTAHV
PNPRRLAVGLEQNRLAMLLAVLHRHAGIACFDQDVFLNAVGGVKITEPAADVAVLLAIHSSMRNKALPKGLIVFGEVGLA
GEIRPSPRGQERLREAAKLGFTSALIPKANAPKQPIEGLNVMAVERIEQAIDRVRDLE

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=660757 MK974_RS10165 WP_011657301.1 2213196..2214572(-) (radA) [Burkholderia ambifaria strain Q53]
GTGGCGAAACAGAAAACCGTCTATGTCTGCAGCGAGTGCGGCGGACAAACCCCGAAGTGGCAAGGGCAATGCCCGTCGTG
CCAGGCCTGGAACACCCTCGTCGAATCGGTCGCCGAGTCGCCGGCCGCCCATCGCTTCCAGTCGCTCGCGAAGCGGGCGC
CGGTGCAGCGGCTCGTCGACATCGAGGCGGCCGACGTGCCGCGCTTCTCCACCGGCATCGGCGAATTCGACCGTGTGCTG
GGTGGCGGGCTGGTGCCGGGCGGCGTCGTGCTGATCGGCGGCGATCCGGGCATCGGCAAGTCGACGCTGCTGCTGCAGTC
GCTCGCGGACATCGCGAACGAGCGGCGCGCGCTCTATATCAGCGGTGAGGAATCGGCCGCGCAGATCGCGTTGCGCGCGC
AACGGCTCGCGCTGCTCGACGGCGGCGCGCCGGCGGCCGAGCTGAAGCTGCTCGCCGAGATCCAGCTCGAGAAGATCCAG
GCCGCGATCGACGCGGAACGGCCGGACGTCGCCGTCATCGACTCGATCCAGACCGTCTATTCGGAAGCGCTGACGTCGGC
GCCCGGCTCGGTCGCGCAGGTGCGCGAATGCGCGGCGCAGCTCACGCGCATCGCGAAGCAGTCCGGCACCGCGATCATCA
TGGTCGGGCACGTGACAAAGGAGGGCAACCTCGCCGGCCCGCGCGTGCTCGAGCACATCGTCGACACGGTGCTGTACTTC
GAGGGCGACACCCATTCGTCGTTCCGGCTCGTGCGCGCGTTCAAGAACCGCTTCGGCGCGGTCAACGAGCTCGGCGTGTT
CGCGATGACCGAGCGCGGGCTGCGCGGCGTCGCGAACCCGTCCGCGCTGTTCCTGTCGCAGCATGAGCAGGTCGTGCCGG
GTTCGTGCGTGCTCGTCACGCAGGAGGGCACGCGCCCGCTGCTCGTCGAAGTCCAGGCGCTCGTCGATACGGCGCACGTG
CCGAACCCGCGCCGGCTCGCGGTCGGCCTCGAGCAGAACCGGCTCGCGATGCTGCTCGCGGTGCTGCACCGGCACGCGGG
CATCGCGTGTTTCGACCAGGACGTGTTCCTGAACGCGGTGGGCGGCGTGAAGATCACCGAACCGGCCGCTGACGTCGCCG
TGCTGCTCGCGATCCATTCGTCGATGCGCAACAAGGCGCTGCCGAAGGGCCTGATCGTATTCGGGGAGGTGGGGCTGGCG
GGCGAGATCCGGCCGTCCCCGCGTGGCCAGGAACGGTTGCGCGAGGCCGCGAAGCTCGGCTTCACGTCTGCGCTGATCCC
GAAGGCGAATGCGCCGAAGCAGCCGATCGAAGGGCTGAACGTGATGGCCGTCGAGCGAATCGAGCAGGCGATCGACCGCG
TGCGCGATCTCGAATGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(78-222)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(363-451)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0BDZ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.024

100

0.493
  radA Streptococcus mitis SK321

47.162

100

0.472
  radA Streptococcus pneumoniae Rx1

46.855

100

0.472
  radA Streptococcus pneumoniae D39

46.855

100

0.472
  radA Streptococcus pneumoniae R6

46.855

100

0.472
  radA Streptococcus pneumoniae TIGR4

46.855

100

0.472
  radA Streptococcus mitis NCTC 12261

46.827

99.782

0.467