Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   MLD62_RS22020 Genome accession   NZ_CP092833
Coordinates   4306396..4307142 (-) Length   248 a.a.
NCBI ID   WP_002088669.1    Uniprot ID   R8MN78
Organism   Bacillus mycoides strain LX30     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4301396..4312142
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MLD62_RS22000 dnaG 4302123..4303925 (-) 1803 WP_002067179.1 DNA primase -
  MLD62_RS22005 - 4304007..4304150 (-) 144 WP_162836445.1 hypothetical protein -
  MLD62_RS22010 - 4304815..4305627 (-) 813 WP_241138622.1 pyruvate, water dikinase regulatory protein -
  MLD62_RS22015 - 4305657..4306289 (-) 633 WP_088034669.1 helix-turn-helix transcriptional regulator -
  MLD62_RS22020 recO 4306396..4307142 (-) 747 WP_002088669.1 DNA repair protein RecO Machinery gene
  MLD62_RS22025 - 4307176..4307319 (-) 144 WP_000883924.1 YqzL family protein -
  MLD62_RS22030 era 4307496..4308401 (-) 906 WP_002088672.1 GTPase Era -
  MLD62_RS22035 - 4308394..4308792 (-) 399 WP_002015184.1 cytidine deaminase -
  MLD62_RS22040 - 4308892..4309245 (-) 354 WP_002143170.1 diacylglycerol kinase family protein -
  MLD62_RS22045 ybeY 4309242..4309712 (-) 471 WP_002165062.1 rRNA maturation RNase YbeY -
  MLD62_RS22050 - 4309709..4311853 (-) 2145 WP_085312392.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28649.18 Da        Isoelectric Point: 7.7708

>NTDB_id=660725 MLD62_RS22020 WP_002088669.1 4306396..4307142(-) (recO) [Bacillus mycoides strain LX30]
MFQKVEGIVIRTTDYGETNKIVTIYSREFGKVSAMARGAKKPKSRLAAISQLMTHGHFLIQMGSGLGTLQQGEIISSMKE
IREDIFLTAYASFIIELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMHPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDPYRIPVGEAVHKLLRLFFHFDLHRLGNVSVKDSTKKQMRTVLNTYYDEYCGIYLKTRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=660725 MLD62_RS22020 WP_002088669.1 4306396..4307142(-) (recO) [Bacillus mycoides strain LX30]
ATGTTTCAAAAAGTTGAGGGCATTGTTATCCGTACGACAGATTACGGAGAAACAAATAAGATTGTTACAATATACTCACG
GGAATTTGGTAAGGTAAGTGCAATGGCAAGAGGGGCGAAAAAGCCGAAGAGTCGGTTAGCAGCTATTTCGCAACTTATGA
CACATGGTCATTTTCTTATTCAAATGGGATCTGGACTTGGAACTTTGCAACAAGGCGAGATTATTTCATCTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTATCGAATTAACTGATAAAGCAACAGAAGATAAGAAGCA
TAATCCGTATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGACCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCACCCGTACTTTGATACATGTGCTATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTCAGGGAAGGCGGTTTTCTGTGCTCTCGTCATGCGGAACAAGATCCGTATCGTATTCCGGTGGG
AGAGGCTGTTCATAAATTATTACGTCTCTTTTTTCATTTTGATTTACACCGACTCGGTAATGTATCGGTGAAGGATAGTA
CAAAAAAACAAATGCGCACAGTATTGAATACATATTATGATGAATATTGTGGGATTTATTTGAAAACAAGGCGTTTCTTG
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8MN78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552