Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MLD62_RS21745 Genome accession   NZ_CP092833
Coordinates   4251790..4252833 (-) Length   347 a.a.
NCBI ID   WP_002014996.1    Uniprot ID   R8I3I9
Organism   Bacillus mycoides strain LX30     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4246790..4257833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MLD62_RS21700 - 4247199..4247399 (-) 201 WP_002015009.1 YqzE family protein -
  MLD62_RS21705 aroK 4247438..4247935 (-) 498 WP_137057331.1 shikimate kinase AroK -
  MLD62_RS21710 - 4248077..4248727 (-) 651 WP_201031782.1 2OG-Fe(II) oxygenase -
  MLD62_RS21715 comGG 4248901..4249272 (-) 372 WP_016096113.1 competence type IV pilus minor pilin ComGG -
  MLD62_RS21720 comGF 4249269..4249730 (-) 462 WP_002088620.1 competence type IV pilus minor pilin ComGF -
  MLD62_RS21725 - 4249714..4250010 (-) 297 WP_002088621.1 hypothetical protein -
  MLD62_RS21730 comGD 4250003..4250458 (-) 456 WP_137059084.1 competence type IV pilus minor pilin ComGD -
  MLD62_RS21735 comGC 4250455..4250754 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  MLD62_RS21740 comGB 4250766..4251803 (-) 1038 WP_016096112.1 competence type IV pilus assembly protein ComGB -
  MLD62_RS21745 comGA 4251790..4252833 (-) 1044 WP_002014996.1 competence type IV pilus ATPase ComGA Machinery gene
  MLD62_RS21750 - 4253039..4253734 (+) 696 WP_002014995.1 helix-turn-helix domain-containing protein -
  MLD62_RS21755 - 4254015..4254257 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  MLD62_RS21760 - 4254365..4255759 (+) 1395 WP_002138338.1 L-cystine transporter -
  MLD62_RS21765 - 4255840..4256232 (-) 393 WP_002138339.1 hypothetical protein -
  MLD62_RS21770 - 4256320..4256535 (-) 216 WP_002015115.1 DUF3912 family protein -
  MLD62_RS21775 - 4256788..4257099 (+) 312 WP_002165070.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39264.73 Da        Isoelectric Point: 9.3368

>NTDB_id=660724 MLD62_RS21745 WP_002014996.1 4251790..4252833(-) (comGA) [Bacillus mycoides strain LX30]
MNSVELFANMIMKEACRVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKWQTRRIITLEDPVEQRKDGLLQIQINEKAGITYKTGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCATLCKSMRKVRQASIYELLYGYELKQAIKEASGEH
VTYHYKTLESSVRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=660724 MLD62_RS21745 WP_002014996.1 4251790..4252833(-) (comGA) [Bacillus mycoides strain LX30]
ATGAATAGTGTCGAGCTTTTTGCAAATATGATTATGAAAGAAGCTTGTAGGGTGCAAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCGATTCAATTACGTATTGGAAAAGATTTAATTACGAAACGGTGTATTGAGAAAGAATTTGGAG
AAAAGCTTGTTTCGCACTTTAAATTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTACAGCCGTTGTCTCACCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCGCATGGGTTACTCGTATTTACTGGGCCGACTGGTTCTGGAAAAACAACAACAATGTATGCATTATTAGAAGTA
GCTAGAAAATGGCAAACACGTCGCATCATTACACTGGAAGATCCAGTTGAGCAAAGAAAAGACGGTTTATTACAAATTCA
AATAAATGAAAAAGCTGGTATCACATATAAAACAGGATTAAAGGCTATTTTGCGTCATGATCCAGATATTATTTTAGTTG
GCGAAATTCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGGGCCAGTTTGACGGGACATTTAGTAATGACAACCTTGCAT
ACAAATGATGCGAAAGGAGCGATACTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTAGC
GGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCATTTTGCAGAGGGAAGTGTGCAACTTTATGTAAATCAATGAGGA
AAGTGAGACAGGCAAGCATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGTGGAGAACAT
GTTACGTATCACTATAAAACGTTGGAATCGTCGGTTCGAAAAGGGTATGCTTTAGGCTTTTTAGAAGAAGATGTATATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R8I3I9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573
  pilB Glaesserella parasuis strain SC1401

43.151

84.15

0.363