Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   MK860_RS12055 Genome accession   NZ_CP092829
Coordinates   2373637..2374407 (-) Length   256 a.a.
NCBI ID   WP_034661344.1    Uniprot ID   -
Organism   Bacillus pumilus strain MS32     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2368637..2379407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK860_RS12035 (MK860_11985) - 2368730..2369551 (-) 822 WP_044139409.1 pyruvate, water dikinase regulatory protein -
  MK860_RS12040 (MK860_11990) - 2369577..2370215 (-) 639 WP_034620854.1 helix-turn-helix transcriptional regulator -
  MK860_RS12045 (MK860_11995) glyS 2370382..2372427 (-) 2046 WP_265208158.1 glycine--tRNA ligase subunit beta -
  MK860_RS12050 (MK860_12000) glyQ 2372420..2373307 (-) 888 WP_003217487.1 glycine--tRNA ligase subunit alpha -
  MK860_RS12055 (MK860_12005) recO 2373637..2374407 (-) 771 WP_034661344.1 DNA repair protein RecO Machinery gene
  MK860_RS12060 (MK860_12010) - 2374442..2374585 (-) 144 WP_065098441.1 YqzL family protein -
  MK860_RS12065 (MK860_12015) era 2374734..2375639 (-) 906 WP_065098416.1 GTPase Era -
  MK860_RS12070 (MK860_12020) - 2375632..2376030 (-) 399 WP_003217296.1 cytidine deaminase -
  MK860_RS12075 (MK860_12025) - 2376144..2376530 (-) 387 WP_044139413.1 diacylglycerol kinase family protein -
  MK860_RS12080 (MK860_12030) ybeY 2376508..2376987 (-) 480 WP_044139414.1 rRNA maturation RNase YbeY -
  MK860_RS12085 (MK860_12035) - 2376988..2379075 (-) 2088 WP_050943969.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29244.92 Da        Isoelectric Point: 8.4348

>NTDB_id=660602 MK860_RS12055 WP_034661344.1 2373637..2374407(-) (recO) [Bacillus pumilus strain MS32]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRT
IREDLFLTAYAAYMTELLDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYEEYSGLYLKSKKFM
NQMESMKKLMGGENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=660602 MK860_RS12055 WP_034661344.1 2373637..2374407(-) (recO) [Bacillus pumilus strain MS32]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGGAAAATTGGTGTGATGGCAAGAGGCGCAAAAAAATCAAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGAACCTTCCTGATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGAACC
ATTCGGGAGGATTTATTCTTGACCGCATATGCCGCATATATGACAGAGTTACTCGATAAAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTCCTGCTTCAATCTTTGCGTCATCTGAATGAAGGCACAGATGCTGATATTATCTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTGATGGGAATGAAGCCCGAACTGGATCAATGTGTCCACTGTGGTCAAAAAGAAGGG
CAGTTTCATTTCTCGATTAGAGACAACGGCTTTATTTGCCAAAATTGCTTTTCTAAAGATCCGTATAAACTGCCCTTATC
TCCAGCAGCTGCAAGGCTGCTCCGTTTATTTCATTATTTTGATTTATCAAGGCTCGGTCAGGTTGATGTGAAACCGCAGA
CGAAACAAGAAATTCGTCAAGTGCTCGATCACTACTATGAAGAGTATTCAGGCCTTTATTTGAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTCATGGGTGGGGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.781

100

0.758