Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   MK860_RS10535 Genome accession   NZ_CP092829
Coordinates   2101880..2102491 (+) Length   203 a.a.
NCBI ID   WP_065098285.1    Uniprot ID   -
Organism   Bacillus pumilus strain MS32     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2096880..2107491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK860_RS10495 (MK860_10450) - 2097043..2098161 (+) 1119 WP_278175400.1 NADH-dependent flavin oxidoreductase -
  MK860_RS10500 (MK860_10455) - 2098214..2098720 (-) 507 WP_003216086.1 PTS glucose transporter subunit IIA -
  MK860_RS10505 (MK860_10460) - 2098888..2099307 (+) 420 WP_003215994.1 Hsp20/alpha crystallin family protein -
  MK860_RS10510 (MK860_10465) - 2099328..2099783 (-) 456 WP_113767350.1 YppG family protein -
  MK860_RS10515 (MK860_10470) - 2099890..2100261 (-) 372 WP_044141136.1 YppE family protein -
  MK860_RS10520 (MK860_10475) - 2100319..2100555 (-) 237 WP_044141135.1 DUF5446 family protein -
  MK860_RS10525 (MK860_10480) sspM 2100749..2100853 (+) 105 WP_012010346.1 acid-soluble spore protein SspM -
  MK860_RS10530 (MK860_10485) - 2100877..2101833 (-) 957 WP_185108933.1 DUF2515 domain-containing protein -
  MK860_RS10535 (MK860_10490) recU 2101880..2102491 (+) 612 WP_065098285.1 Holliday junction resolvase RecU Machinery gene
  MK860_RS10540 (MK860_10495) - 2102535..2105261 (+) 2727 WP_268453952.1 PBP1A family penicillin-binding protein -
  MK860_RS10545 (MK860_10500) - 2105298..2105807 (-) 510 WP_065098287.1 YpoC family protein -
  MK860_RS10550 (MK860_10505) nth 2105804..2106466 (-) 663 WP_034661035.1 endonuclease III -
  MK860_RS10555 (MK860_10510) - 2106484..2107188 (-) 705 WP_065098288.1 DnaD domain-containing protein -

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 23203.32 Da        Isoelectric Point: 8.6102

>NTDB_id=660596 MK860_RS10535 WP_065098285.1 2101880..2102491(+) (recU) [Bacillus pumilus strain MS32]
MIRYPNGKSYQPIQPIGTKKGISGESSYSNRGMTLEADLNETNQYYLVNGIAVIHKKPTPVQIVNVDYPKRSAAVIKEAY
FKQSSTTDYNGVYKGRYIDFEAKETKSTTSFPLKNFHDHQIEHMKQVEKQGGICFVIISAFGSVYYLPASDLFFFWERQK
LNGRKSISKDELMEAGHLMTLGYSPRIDYIKVVETLHFSDESE

Nucleotide


Download         Length: 612 bp        

>NTDB_id=660596 MK860_RS10535 WP_065098285.1 2101880..2102491(+) (recU) [Bacillus pumilus strain MS32]
ATGATTCGGTATCCAAACGGAAAATCGTATCAGCCCATTCAACCAATTGGGACAAAAAAAGGAATCAGCGGCGAATCCTC
TTATAGTAACCGCGGGATGACGCTTGAGGCTGACTTAAATGAAACCAACCAGTACTATTTGGTCAACGGGATCGCAGTCA
TTCATAAAAAGCCTACCCCCGTTCAAATTGTGAATGTGGATTATCCAAAAAGAAGCGCTGCTGTGATCAAAGAAGCCTAT
TTTAAACAATCATCCACAACAGACTATAATGGAGTGTACAAAGGGCGCTATATCGATTTTGAAGCAAAGGAAACCAAAAG
CACTACCTCGTTTCCGCTCAAAAATTTTCACGACCATCAAATTGAGCATATGAAACAGGTTGAAAAGCAAGGCGGTATTT
GTTTCGTTATTATATCCGCATTTGGCTCCGTTTATTATTTGCCCGCTTCAGATCTCTTTTTCTTCTGGGAGCGGCAGAAG
CTAAACGGCCGGAAATCCATTAGTAAAGACGAATTAATGGAAGCCGGCCACTTAATGACACTTGGATATTCGCCAAGAAT
TGATTATATTAAAGTAGTGGAGACACTTCATTTTTCGGATGAAAGTGAGTAG

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

72.772

99.507

0.724