Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   MK860_RS07840 Genome accession   NZ_CP092829
Coordinates   1587292..1588194 (+) Length   300 a.a.
NCBI ID   WP_065097271.1    Uniprot ID   -
Organism   Bacillus pumilus strain MS32     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1582292..1593194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK860_RS07820 (MK860_07800) - 1582958..1584694 (+) 1737 WP_268508243.1 glycosyltransferase -
  MK860_RS07825 (MK860_07805) - 1584691..1584966 (+) 276 WP_065097270.1 FlhB-like flagellar biosynthesis protein -
  MK860_RS07830 (MK860_07810) sucC 1585143..1586303 (+) 1161 WP_012009945.1 ADP-forming succinate--CoA ligase subunit beta -
  MK860_RS07835 (MK860_07815) sucD 1586326..1587228 (+) 903 WP_003212080.1 succinate--CoA ligase subunit alpha -
  MK860_RS07840 (MK860_07820) dprA 1587292..1588194 (+) 903 WP_065097271.1 DNA-processing protein DprA Machinery gene
  MK860_RS07845 (MK860_07825) topA 1588388..1590463 (+) 2076 WP_044141928.1 type I DNA topoisomerase -
  MK860_RS07850 (MK860_07830) trmFO 1590534..1591838 (+) 1305 WP_003212444.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  MK860_RS07855 (MK860_07835) xerC 1591901..1592818 (+) 918 WP_003211704.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34133.60 Da        Isoelectric Point: 9.1124

>NTDB_id=660591 MK860_RS07840 WP_065097271.1 1587292..1588194(+) (dprA) [Bacillus pumilus strain MS32]
MYNVSERMIFHRLKGLISPSLLTKWWKVDPKLYINEETHHFKQDRSLQTIDFTRLKQAEEKEFPIFQHIVQAYLKQNIHM
IPITSPLYPSTLKHIYDPPPVLFLKGNVSYLNEEKSLGVVGTRVPSSYGEACVKKIVGELVKEDWMIVSGLAKGIDGLAH
KECIRNKGKTIGIIAGGFQHLYPKEHVQMAEYMGEHHLLLSEHPPYVKPEKWHFPLRNRLISALTSGTIVIQCKEKSGSL
ITAYQALEQGKEVFAVAGSIFDPNSTGPARLIQQGAKLVHSTKDILEEFSFRSVQYTELL

Nucleotide


Download         Length: 903 bp        

>NTDB_id=660591 MK860_RS07840 WP_065097271.1 1587292..1588194(+) (dprA) [Bacillus pumilus strain MS32]
ATGTACAACGTGTCCGAAAGAATGATTTTTCACCGCTTAAAAGGCCTCATCTCACCCTCTTTGTTAACAAAATGGTGGAA
AGTCGATCCTAAGCTATATATAAATGAAGAAACACATCATTTCAAACAGGATCGATCATTACAAACAATCGACTTTACCC
GCTTAAAACAAGCCGAAGAAAAGGAATTCCCCATTTTTCAACACATCGTTCAAGCCTATTTAAAGCAAAACATTCACATG
ATTCCCATCACATCACCCTTATATCCCAGCACACTAAAACATATTTATGATCCTCCCCCTGTGTTATTCCTAAAAGGAAA
CGTGTCATATTTAAATGAAGAAAAAAGTTTAGGTGTAGTAGGCACGCGTGTTCCATCGTCTTATGGAGAAGCATGTGTGA
AGAAAATTGTTGGTGAACTTGTTAAGGAAGATTGGATGATTGTCAGTGGCTTAGCAAAAGGCATTGATGGACTTGCACAT
AAAGAGTGCATTAGGAATAAAGGGAAAACGATCGGTATTATAGCAGGCGGATTTCAGCATTTATACCCAAAGGAACATGT
GCAAATGGCTGAATACATGGGCGAGCATCATTTGCTTTTATCCGAGCATCCGCCTTATGTCAAACCAGAAAAGTGGCATT
TCCCTTTGAGGAATCGTTTAATTAGTGCGCTGACAAGTGGGACCATCGTGATTCAGTGCAAAGAAAAGAGCGGTTCACTC
ATTACCGCGTATCAAGCACTTGAGCAAGGCAAAGAGGTATTTGCGGTTGCAGGATCAATCTTTGATCCTAATTCCACAGG
TCCAGCCAGACTTATACAGCAGGGAGCAAAGCTTGTTCATTCAACGAAGGATATTTTAGAGGAATTCTCCTTCCGCAGCG
TTCAATATACTGAACTCTTATAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

54.704

95.667

0.523
  dprA Lactococcus lactis subsp. cremoris KW2

38.542

96

0.37