Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MK860_RS01935 Genome accession   NZ_CP092829
Coordinates   358480..358908 (-) Length   142 a.a.
NCBI ID   WP_044140469.1    Uniprot ID   A0AB34QU23
Organism   Bacillus pumilus strain MS32     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 353480..363908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK860_RS01895 (MK860_01895) - 353603..354475 (-) 873 WP_185108286.1 transporter substrate-binding domain-containing protein -
  MK860_RS01900 (MK860_01900) - 354656..354979 (+) 324 WP_044140620.1 YckD family protein -
  MK860_RS01905 (MK860_01905) - 355035..355799 (-) 765 WP_044140464.1 MBL fold metallo-hydrolase -
  MK860_RS01910 (MK860_01910) - 355915..356331 (+) 417 WP_044140465.1 Lrp/AsnC family transcriptional regulator -
  MK860_RS01915 (MK860_01915) - 356360..357166 (+) 807 WP_065098771.1 AAC(3) family N-acetyltransferase -
  MK860_RS01920 (MK860_01920) - 357168..357554 (-) 387 WP_113768527.1 competence protein ComJ -
  MK860_RS01925 (MK860_01925) - 357675..358163 (-) 489 WP_231129532.1 methyl-accepting chemotaxis protein -
  MK860_RS01930 (MK860_01930) - 358103..358249 (-) 147 WP_231129527.1 hypothetical protein -
  MK860_RS01935 (MK860_01935) nucA/comI 358480..358908 (-) 429 WP_044140469.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MK860_RS01940 (MK860_01940) - 359276..361345 (+) 2070 WP_126741562.1 hydantoinase/oxoprolinase family protein -
  MK860_RS01945 (MK860_01945) - 361335..363320 (+) 1986 WP_278177752.1 hydantoinase B/oxoprolinase family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15927.84 Da        Isoelectric Point: 5.5491

>NTDB_id=660570 MK860_RS01935 WP_044140469.1 358480..358908(-) (nucA/comI) [Bacillus pumilus strain MS32]
MKLLKIILLLLLIVVGVATGYIQLEQSKQETTNSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSVCTIDRKHSDEQREQS
LHGIPTKRGYDRDEWPMAMCKEGGTGASVKYINPSDNRGAGSWVGHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=660570 MK860_RS01935 WP_044140469.1 358480..358908(-) (nucA/comI) [Bacillus pumilus strain MS32]
ATGAAACTTCTTAAAATCATTCTCTTGCTTTTACTGATCGTCGTTGGTGTAGCAACAGGCTATATTCAGCTGGAGCAAAG
TAAACAAGAGACGACGAATTCATCTTATGACAAAACGATTCACTTCCCATCAGATCGTTACCCCGAAACCGCTAAGCACA
TAGAAGAGGCCATTGATGAAGGCCATTCATCCGTATGTACGATTGACCGTAAACATAGCGATGAACAAAGAGAACAATCA
CTTCATGGCATCCCAACAAAGCGCGGGTATGATCGGGACGAATGGCCGATGGCGATGTGTAAGGAAGGAGGAACAGGAGC
TTCAGTCAAATATATTAATCCTTCAGATAATAGAGGAGCAGGCTCATGGGTCGGTCATCAATTATCGGATGATCCTGACG
GCACAAGAATACAATTTATCATCGATTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.116

85.211

0.563