Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MK860_RS00590 Genome accession   NZ_CP092829
Coordinates   109216..110595 (+) Length   459 a.a.
NCBI ID   WP_278177481.1    Uniprot ID   -
Organism   Bacillus pumilus strain MS32     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 104216..115595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK860_RS00570 (MK860_00570) - 104558..105022 (+) 465 WP_003217184.1 CtsR family transcriptional regulator -
  MK860_RS00575 (MK860_00575) - 105037..105594 (+) 558 WP_034620770.1 UvrB/UvrC motif-containing protein -
  MK860_RS00580 (MK860_00580) - 105599..106690 (+) 1092 WP_065097645.1 protein arginine kinase -
  MK860_RS00585 (MK860_00585) clpC 106687..109122 (+) 2436 WP_034665649.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  MK860_RS00590 (MK860_00590) radA 109216..110595 (+) 1380 WP_278177481.1 DNA repair protein RadA Machinery gene
  MK860_RS00595 (MK860_00595) disA 110598..111677 (+) 1080 WP_065097644.1 DNA integrity scanning diadenylate cyclase DisA -
  MK860_RS00600 (MK860_00600) - 111831..112931 (+) 1101 WP_003217214.1 PIN/TRAM domain-containing protein -
  MK860_RS00605 (MK860_00605) ispD 112945..113634 (+) 690 WP_034665643.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MK860_RS00610 (MK860_00610) ispF 113638..114114 (+) 477 WP_003216928.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50038.57 Da        Isoelectric Point: 7.9305

>NTDB_id=660569 MK860_RS00590 WP_278177481.1 109216..110595(+) (radA) [Bacillus pumilus strain MS32]
MAKTKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPANRRSAFNHSVQTIQKPSPISAIETSDEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDKNQNVLYISGEESIKQTKLRADRLGIKSTSLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLTEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANIEGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=660569 MK860_RS00590 WP_278177481.1 109216..110595(+) (radA) [Bacillus pumilus strain MS32]
ATGGCTAAGACAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGTCCAGGCTG
CGGCACGTGGAACAGTATGACAGAAGAGGTCGTTCGCAAAGAGCCGGCAAACCGTCGAAGTGCTTTTAATCATTCTGTTC
AAACCATTCAAAAACCTTCACCTATATCTGCAATTGAAACATCTGATGAACCCCGAATCAAAACGAATTTAGAAGAATTT
AACCGAGTATTAGGAAGTGGAATTGTCAAAGGCTCTCTTGTTCTCATTGGCGGAGATCCTGGGATTGGGAAGTCCACATT
ATTATTACAAGTATCAGCACAGCTCTCAGACAAAAATCAGAATGTATTATACATATCTGGTGAGGAGTCCATTAAACAAA
CGAAGCTAAGAGCGGACCGCCTTGGCATAAAAAGCACCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTGCTATACAAGAGATGAAACCCGCTTTTGTCGTGGTGGATTCGATTCAAACCGTTTATCAAAGCGATATTACGTCAGC
TCCTGGGTCTGTATCTCAAGTGAGAGAATGTACAGCACAGCTGATGAAGATTGCCAAGACAAATGGTATTCCAATATTTA
TCGTTGGTCACGTGACCAAAGAAGGCTCGATCGCAGGTCCACGCCTTTTAGAGCATATGGTGGACACGGTTCTTTATTTT
GAAGGCGAGCGTCACCATACGTTTCGTATCTTGCGTGCAGTGAAAAACCGATTTGGTTCGACGAATGAACTAGGGATTTT
TGAAATGAGAGAGGAAGGGCTCACGGAAGTATTAAACCCATCCGAAATTTTCTTAGAAGAACGTTCGGCAGGTGTATCTG
GTTCGTGTGTTGTTGCCTCAATGGAAGGAACAAGACCTGTTCTTGTCGAGATACAAGCATTAATTTCACCGACAAGCTTC
GGAAATCCGCGTAGAATGGCCACTGGTCTTGATCATAATCGAGTGTCACTACTCATGGCGGTTTTAGAAAAACGTGTTGG
ACTACTGCTGCAAAACCAGGATGCGTATTTAAAGGTCGCAGGCGGTGTGAAGCTTGACGAACCAGCAATTGACTTGGCCA
TTGCGGTCAGCATTGCATCGAGCTTTAAAGACGCAGCGCCGCATCAAGCGGATTGCTTTATAGGAGAGGTCGGTTTGACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAGCGTGTGCAGGAAGCTGCGAAACTAGGATTTAAGCGAATGTTTATTCC
TCAGGCGAATATAGAGGGATGGAAAAAGCCGAGAGGGATTGAGTTAGTCGGTGTAGAAAATGTAGCGGAGGCACTTCGAA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

86.9

99.782

0.867
  radA Streptococcus mitis NCTC 12261

61.589

98.693

0.608
  radA Streptococcus pneumoniae Rx1

61.589

98.693

0.608
  radA Streptococcus pneumoniae D39

61.589

98.693

0.608
  radA Streptococcus pneumoniae R6

61.589

98.693

0.608
  radA Streptococcus pneumoniae TIGR4

61.589

98.693

0.608
  radA Streptococcus mitis SK321

61.589

98.693

0.608