Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   MK860_RS00020 Genome accession   NZ_CP092829
Coordinates   3057..4169 (+) Length   370 a.a.
NCBI ID   WP_044139026.1    Uniprot ID   A0AB34R1W9
Organism   Bacillus pumilus strain MS32     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK860_RS00005 (MK860_00005) dnaA 1..1341 (+) 1341 WP_003214781.1 chromosomal replication initiator protein DnaA -
  MK860_RS00010 (MK860_00010) dnaN 1538..2674 (+) 1137 WP_044139028.1 DNA polymerase III subunit beta -
  MK860_RS00015 (MK860_00015) yaaA 2825..3040 (+) 216 WP_003215257.1 S4 domain-containing protein YaaA -
  MK860_RS00020 (MK860_00020) recF 3057..4169 (+) 1113 WP_044139026.1 DNA replication/repair protein RecF Machinery gene
  MK860_RS00025 (MK860_00025) - 4187..4432 (+) 246 WP_041815080.1 extracellular matrix/biofilm biosynthesis regulator RemA family protein -
  MK860_RS00030 (MK860_00030) gyrB 4490..6406 (+) 1917 WP_065097624.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  MK860_RS00035 (MK860_00035) gyrA 6637..9138 (+) 2502 WP_065097564.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42609.70 Da        Isoelectric Point: 7.7388

>NTDB_id=660565 MK860_RS00020 WP_044139026.1 3057..4169(+) (recF) [Bacillus pumilus strain MS32]
MYIQSLALTSYRNYEHTELQFDNKVNVMIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDQDYAKIEGRVIKKNGP
LPMQLVISKKGKRGKVNHIEQQKLSHYVGALNTIMFAPEDLSLVKGSPQIRRRFLDMEIGQVSAVYLHDLSLYQKILSQR
NHYLKQLQTRKQKDQAMLEVLTEQLIDAAAKVVKRRLTFTKQLEKWAQPLHFGISRELETLTLQYHTAIEVSEASDLSKI
KNSYEESFQKLRDREIDRGVTLWGPHRDDLLFFVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVEGIDHATLKEAEIFRVASGKVID

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=660565 MK860_RS00020 WP_044139026.1 3057..4169(+) (recF) [Bacillus pumilus strain MS32]
ATGTACATTCAAAGTCTGGCGTTAACTTCATACCGAAACTATGAACACACCGAGCTTCAATTCGACAACAAGGTGAATGT
CATGATCGGTGAGAATGCCCAAGGTAAAACGAACTTGATGGAAGCGATCTATGTATTGTCGATGGCAAAGTCGCATCGTA
CGTCAAATGATAAAGAACTTATCCGATGGGACCAAGACTATGCTAAAATAGAAGGTAGAGTCATCAAAAAAAATGGTCCA
CTCCCAATGCAGCTCGTGATTTCAAAAAAAGGGAAAAGGGGCAAGGTCAATCACATTGAACAACAGAAGCTCAGTCATTA
TGTTGGTGCGTTAAACACCATCATGTTTGCACCAGAGGACTTAAGTCTTGTAAAGGGCAGCCCGCAAATCCGCAGAAGAT
TTCTCGATATGGAGATTGGACAAGTTTCTGCTGTCTACTTGCATGATTTATCGCTCTATCAAAAAATTCTCTCTCAGCGG
AATCATTACTTGAAACAATTGCAGACAAGAAAGCAAAAGGATCAAGCGATGCTGGAGGTTTTAACAGAGCAGTTGATTGA
TGCGGCAGCGAAAGTTGTCAAAAGACGACTGACTTTTACGAAACAGCTTGAAAAATGGGCGCAGCCGTTGCATTTTGGCA
TTTCTAGAGAGCTAGAAACACTCACGCTCCAATACCACACGGCGATAGAGGTATCAGAAGCGTCAGACTTGTCGAAAATA
AAGAATAGCTATGAAGAATCGTTTCAGAAACTAAGAGACAGAGAAATAGACCGAGGAGTGACGCTGTGGGGACCTCACAG
AGATGACCTTCTTTTCTTTGTGAATGGTCGGGATGTTCAGACATATGGCTCTCAAGGGCAGCAAAGAACAACAGCTCTTT
CACTAAAGCTGGCAGAAATCGACTTGATACACGAAGAGATTGGAGAATATCCCATTCTTCTACTCGATGATGTTTTATCT
GAACTTGATGATTACAGACAGTCTCATTTGCTCCATACCATTCAGGGACGTGTACAGACCTTCGTCACCACAACAAGTGT
TGAAGGCATCGATCACGCCACCTTGAAAGAAGCGGAAATTTTCAGAGTAGCCAGTGGAAAAGTAATTGACTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

83.469

99.73

0.832