Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MK848_RS05150 Genome accession   NZ_CP092824
Coordinates   1035926..1036336 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain S1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1030926..1041336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK848_RS05120 (MK848_05125) - 1031013..1031153 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  MK848_RS05125 (MK848_05130) yqeF 1031459..1032190 (-) 732 WP_101172379.1 SGNH/GDSL hydrolase family protein -
  MK848_RS05130 (MK848_05135) cwlH 1032442..1033194 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  MK848_RS05135 (MK848_05140) yqeD 1033381..1034007 (+) 627 WP_101172378.1 TVP38/TMEM64 family protein -
  MK848_RS05140 (MK848_05145) gnd 1034026..1034919 (-) 894 WP_101172377.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  MK848_RS05145 (MK848_05150) yqeB 1035171..1035893 (+) 723 WP_010886572.1 hypothetical protein -
  MK848_RS05150 (MK848_05155) nucA/comI 1035926..1036336 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  MK848_RS05155 (MK848_05160) - 1036532..1036882 (+) 351 Protein_1026 sigma-70 family RNA polymerase sigma factor -
  MK848_RS05160 (MK848_05165) spoIVCA 1036910..1038370 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  MK848_RS05165 (MK848_05170) - 1038328..1038506 (-) 179 Protein_1028 hypothetical protein -
  MK848_RS05170 (MK848_05175) arsC 1038861..1039280 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  MK848_RS05175 (MK848_05180) acr3 1039292..1040332 (-) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  MK848_RS05180 (MK848_05185) arsK 1040355..1040795 (-) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  MK848_RS05185 (MK848_05190) arsR 1040854..1041171 (-) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=660420 MK848_RS05150 WP_009967785.1 1035926..1036336(-) (nucA/comI) [Bacillus subtilis strain S1]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=660420 MK848_RS05150 WP_009967785.1 1035926..1036336(-) (nucA/comI) [Bacillus subtilis strain S1]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529