Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BRPE67_RS01260 Genome accession   NZ_AP014576
Coordinates   253300..254817 (-) Length   505 a.a.
NCBI ID   WP_045450299.1    Uniprot ID   A0A158EUL2
Organism   Caballeronia cordobensis strain RPE67     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 248300..259817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BRPE67_RS01235 (BRPE67_ACDS02420) - 249093..249725 (-) 633 WP_014190332.1 LON peptidase substrate-binding domain-containing protein -
  BRPE67_RS01240 (BRPE67_ACDS02430) rapZ 249785..250657 (-) 873 WP_014190333.1 RNase adapter RapZ -
  BRPE67_RS01245 (BRPE67_ACDS02440) hprK 250739..251707 (-) 969 WP_008351692.1 HPr(Ser) kinase/phosphatase -
  BRPE67_RS01250 (BRPE67_ACDS02450) ptsN 251893..252348 (-) 456 WP_045451816.1 PTS IIA-like nitrogen regulatory protein PtsN -
  BRPE67_RS42095 - 252381..252605 (-) 225 WP_144403865.1 hypothetical protein -
  BRPE67_RS01255 (BRPE67_ACDS02460) hpf 252756..253115 (-) 360 WP_014190335.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BRPE67_RS01260 (BRPE67_ACDS02470) rpoN/rpoN1 253300..254817 (-) 1518 WP_045450299.1 RNA polymerase factor sigma-54 Machinery gene
  BRPE67_RS01265 (BRPE67_ACDS02480) lptB 254974..255741 (-) 768 WP_085952622.1 LPS export ABC transporter ATP-binding protein -
  BRPE67_RS01270 (BRPE67_ACDS02490) lptA 255753..256418 (-) 666 WP_045450300.1 lipopolysaccharide transport periplasmic protein LptA -
  BRPE67_RS01275 (BRPE67_ACDS02500) lptC 256448..257050 (-) 603 WP_014190339.1 LPS export ABC transporter periplasmic protein LptC -
  BRPE67_RS01280 (BRPE67_ACDS02510) - 257055..257594 (-) 540 WP_045450301.1 KdsC family phosphatase -
  BRPE67_RS01285 (BRPE67_ACDS02520) kdsD 257594..258577 (-) 984 WP_014190341.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55576.65 Da        Isoelectric Point: 4.8824

>NTDB_id=66028 BRPE67_RS01260 WP_045450299.1 253300..254817(-) (rpoN/rpoN1) [Caballeronia cordobensis strain RPE67]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDDWIASPLRVAADGSLIAQQGAPSTPSEPM
MSNGSTSSTSSSERSESSEPQGVDEYNGLGSDSGQDSSSWNLEDYGRANNASDDDDLPPLQIHESTTTLRDHLTAQLRMT
AANQRDRALVTFLIESLDEDGYLTSSLDEVQTDLPEELEVEIEELSAALALLQSFDPPGVGGRSASECLKLQLLRLDGSP
TRTLALEIVMNHLELLAARDFTRLRKQLKASDDSLRDAHLLIKSLEPFPGAAYGKSEADYVVPDILVRKTASGWTAELNP
EIVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDTKTPLSDSRIAELLAEQGF
VVARRTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=66028 BRPE67_RS01260 WP_045450299.1 253300..254817(-) (rpoN/rpoN1) [Caballeronia cordobensis strain RPE67]
ATGAAAGCCAGCCTCCAACTCCGCCTCTCGCAGCATCTTGCGCTGACCCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCTACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCCCAGAACCCGCTGCTCGAGAACGACGACGACTGGA
TCGCGAGCCCGCTGCGCGTGGCCGCCGACGGCTCGCTGATCGCGCAGCAAGGTGCGCCCAGCACGCCTTCCGAGCCGATG
ATGAGCAACGGCTCGACATCGTCCACGTCGAGTTCCGAACGTTCGGAAAGCAGCGAGCCGCAGGGCGTCGACGAATACAA
CGGGCTGGGTTCGGATAGCGGACAAGATTCCAGTTCGTGGAATCTCGAAGACTACGGCCGCGCGAACAACGCATCCGACG
ACGACGATCTCCCGCCGCTGCAAATCCACGAATCGACCACGACGCTGCGCGACCATCTGACCGCGCAGTTGCGCATGACT
GCGGCGAATCAGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAGTCGCTGGACGAGGACGGGTATCTGACTTCGTCGCT
CGACGAGGTGCAAACCGATCTGCCGGAAGAACTCGAAGTCGAGATCGAAGAACTCAGTGCGGCACTCGCATTGCTGCAAA
GTTTCGATCCGCCCGGCGTCGGCGGGCGATCGGCGTCGGAATGCCTGAAGCTGCAATTGCTGCGGCTCGACGGCTCGCCC
ACGCGCACGCTCGCGCTCGAGATCGTCATGAATCATCTGGAATTGCTCGCGGCGCGCGACTTCACGCGTCTGCGCAAGCA
ACTGAAGGCGAGCGACGACTCCCTCCGGGACGCGCATTTGCTGATCAAGTCGCTGGAGCCATTTCCGGGCGCGGCGTATG
GCAAGAGCGAAGCGGACTACGTGGTGCCGGACATTCTCGTCAGAAAGACCGCGAGCGGATGGACAGCCGAGCTCAATCCG
GAAATCGTGCCGAAGCTGCGCATCAATCATTTGTACGCGAACATTCTGCGCAACAACCGGGGCGATCCCGGCAGTGGCTC
GCTGCGTCAGCAGCTCCAGGAAGCGCGCTGGCTGATCAAGAATATTCAGCAGAGGTTTGAAACGATCCTGCGCGTCGCGC
AGGCGATCGTGGAGCGTCAGAAGAACTTTTTTGCACATGGCGAAATCGCCATGCGGCCCTTGGTTTTGCGGGAGATTGCT
GATACGCTGGGATTACACGAGTCGACGGTTTCTCGTGTGACCACAGGCAAGTACATGCTCACCCCATTCGGGACGCTTGA
ATTTAAGTACTTCTTTGGATCGCACGTTTCGACCGACACAGGAGGCGCGGCATCGTCAACGGCGATTCGCGCACTCATCA
AGCAACTGATAGGAGCGGAAGACACCAAAACTCCTCTTTCAGACAGCCGCATCGCCGAACTGCTGGCGGAGCAAGGATTC
GTGGTCGCGCGGCGAACCGTCGCCAAGTATCGCGAAGCCCTCAGGATCCCCGCAGTGAATCTGCGCAAGTCTCTATGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(142-332)

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A158EUL2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.208

100

0.667

Multiple sequence alignment