Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   MK616_RS18125 Genome accession   NZ_CP092778
Coordinates   3688213..3689358 (+) Length   381 a.a.
NCBI ID   WP_099721980.1    Uniprot ID   A0AAP3YDZ8
Organism   Bacillus amyloliquefaciens strain WF2020     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3683213..3694358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK616_RS18105 (MK616_18105) - 3683688..3684404 (-) 717 WP_014419126.1 Crp/Fnr family transcriptional regulator -
  MK616_RS18110 (MK616_18110) narK 3684502..3685692 (-) 1191 WP_007407632.1 nitrate transporter NarK -
  MK616_RS18115 (MK616_18115) argS 3685826..3687496 (-) 1671 WP_007407633.1 arginine--tRNA ligase -
  MK616_RS18120 (MK616_18120) - 3687493..3687921 (-) 429 WP_014419128.1 DUF1934 domain-containing protein -
  MK616_RS18125 (MK616_18125) rapF 3688213..3689358 (+) 1146 WP_099721980.1 tetratricopeptide repeat protein Regulator
  MK616_RS18130 (MK616_18130) - 3689342..3689461 (+) 120 WP_021494790.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  MK616_RS18135 (MK616_18135) - 3689659..3689862 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  MK616_RS18140 (MK616_18140) - 3689876..3690079 (+) 204 WP_007407641.1 hypothetical protein -
  MK616_RS18145 (MK616_18145) - 3690114..3690299 (+) 186 WP_007407642.1 DUF4177 domain-containing protein -
  MK616_RS18150 (MK616_18150) - 3690486..3690767 (+) 282 WP_007407643.1 hypothetical protein -
  MK616_RS18155 (MK616_18155) - 3690811..3691323 (+) 513 WP_007614439.1 stage II sporulation protein M -
  MK616_RS18160 (MK616_18160) - 3691914..3692132 (-) 219 WP_003151056.1 helix-turn-helix transcriptional regulator -
  MK616_RS18165 (MK616_18165) speB 3692287..3693159 (-) 873 WP_007614448.1 agmatinase -
  MK616_RS18170 (MK616_18170) speE 3693218..3694048 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45567.62 Da        Isoelectric Point: 4.5769

>NTDB_id=660235 MK616_RS18125 WP_099721980.1 3688213..3689358(+) (rapF) [Bacillus amyloliquefaciens strain WF2020]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNTAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKLMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=660235 MK616_RS18125 WP_099721980.1 3688213..3689358(+) (rapF) [Bacillus amyloliquefaciens strain WF2020]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTACGGAGAGAAATCAAAGAAGAACTTGCAGGCCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAACAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAAGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGACTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATACGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAGTATGAATTTTTAGAATCTTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATTGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(189-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.478

100

0.785
  rapC Bacillus subtilis subsp. subtilis str. 168

56.842

99.738

0.567