Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   H7R00_RS01930 Genome accession   NZ_AP022105
Coordinates   427341..428018 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain WP5-S17-ESBL-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422341..433018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R00_RS01915 (WP5S17E10_03850) chiA 423709..426402 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  H7R00_RS01920 (WP5S17E10_03860) bfd 426570..426764 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  H7R00_RS01925 (WP5S17E10_03870) bfr 426836..427312 (+) 477 WP_000675504.1 bacterioferritin -
  H7R00_RS01930 (WP5S17E10_03880) pilD 427341..428018 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  H7R00_RS01935 (WP5S17E10_03890) gspM 428018..428479 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  H7R00_RS01940 (WP5S17E10_03900) gspL 428476..429639 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  H7R00_RS01945 (WP5S17E10_03910) gspK 429654..430637 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  H7R00_RS01950 (WP5S17E10_03920) gspJ 430630..431217 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  H7R00_RS01955 (WP5S17E10_03930) gspI 431210..431587 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  H7R00_RS01960 (WP5S17E10_03940) gspH 431584..432093 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  H7R00_RS01965 (WP5S17E10_03950) gspG 432101..432538 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=66022 H7R00_RS01930 WP_000178161.1 427341..428018(-) (pilD) [Escherichia coli strain WP5-S17-ESBL-10]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=66022 H7R00_RS01930 WP_000178161.1 427341..428018(-) (pilD) [Escherichia coli strain WP5-S17-ESBL-10]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment