Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MK616_RS13180 Genome accession   NZ_CP092778
Coordinates   2701277..2703637 (-) Length   786 a.a.
NCBI ID   WP_100261918.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain WF2020     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2696277..2708637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK616_RS13160 (MK616_13160) - 2696349..2697914 (+) 1566 WP_240680067.1 SH3 domain-containing protein -
  MK616_RS13165 (MK616_13165) dtd 2697941..2698384 (-) 444 WP_007408194.1 D-aminoacyl-tRNA deacylase -
  MK616_RS13170 (MK616_13170) relA 2698397..2700601 (-) 2205 WP_003152716.1 GTP diphosphokinase -
  MK616_RS13175 (MK616_13175) - 2700759..2701271 (-) 513 WP_003152714.1 adenine phosphoribosyltransferase -
  MK616_RS13180 (MK616_13180) recJ 2701277..2703637 (-) 2361 WP_100261918.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MK616_RS13185 (MK616_13185) - 2703693..2704019 (-) 327 WP_014418528.1 LapA family protein -
  MK616_RS13190 (MK616_13190) - 2704083..2704580 (-) 498 WP_014418529.1 cation:proton antiporter regulatory subunit -
  MK616_RS13195 (MK616_13195) secDF 2704712..2706931 (-) 2220 WP_022552888.1 protein translocase subunit SecDF -
  MK616_RS13200 (MK616_13200) - 2706968..2707264 (-) 297 WP_014418531.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 87267.65 Da        Isoelectric Point: 4.9172

>NTDB_id=660216 MK616_RS13180 WP_100261918.1 2701277..2703637(-) (recJ) [Bacillus amyloliquefaciens strain WF2020]
MLASKMRWEIQQPDQEKVKSLTEQLDITPLVASLLVKRGFDTAESARLFLHTKDADFYDPYELKGMREAVDRIKLAVSQG
EPIMIYGDYDADGVTSTSVMLTALRQLSADVDFYIPDRFTEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAEVAKEL
GLDVIITDHHEPGPELPDVFAIIHPKQPGCGYPFKELAGVGVAFKLAHALTGSLPEAMLDLAAIGTVADLVPLHDENRLI
ASLGIERLRTTNRPGLKALIKLSGGDIGQADEETIGFQVAPRLNAVGRIEQADPAVHLLMTEDQFEAEELASDIDLLNKE
RQKMVKTMTEEAVEMVQQQGGNPSAIVVAKAGWNPGVVGIVASKLAERFYRPAVVLGIDEEQGIAKGSARSIRGFNLFTS
LSQCRDILPHFGGHPMAAGMTLKAEDVAELRDRLNQMAEETLTEEDFIPVQEVDLVCGVEDITVESITELNMLSPFGMLN
PKPHVMVKNAVLDDVRLIGANKNHVKMTVKSDSAVLDCVGFNKGELEAGVVPGSTISIVGEMSINEWNNRKKPQLMIKDA
AVPEWQLFDIRGKRTWEDTVTALPKEKRVLISFNEKTAQKLREKELSGELAGITSESEAEALQLDGKYAVFLDVPPSADR
LQHLLDGKKPERIYFVFINHEEHFLSSFPSRNDFKWFYAFLLKRGAFDFGKHGSELARHRGWSIDTINFMTKVFFDLGFV
KIEDGVLSVVSGAKKRDLADSQTYQVKQQLMELDQKLNYSAAHELKEWLNKLMNQGSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=660216 MK616_RS13180 WP_100261918.1 2701277..2703637(-) (recJ) [Bacillus amyloliquefaciens strain WF2020]
ATGTTAGCGTCAAAAATGCGCTGGGAAATCCAGCAGCCGGATCAGGAAAAAGTCAAATCACTCACAGAACAATTGGACAT
AACACCTCTTGTGGCATCACTGCTTGTAAAAAGAGGTTTTGATACTGCCGAAAGCGCAAGGCTGTTTTTACATACAAAGG
ATGCAGACTTTTATGACCCGTATGAATTAAAGGGAATGCGGGAAGCAGTTGATCGGATAAAACTGGCGGTTTCTCAAGGG
GAACCGATTATGATTTACGGAGATTACGATGCAGACGGCGTTACAAGCACGTCAGTGATGCTGACGGCCCTCAGACAGCT
GTCGGCGGACGTTGACTTTTATATTCCCGACCGTTTTACAGAAGGCTACGGCCCGAATGAACAAGCTTTCCGTTCCATAA
AAGAGCGGGGATTTTCGCTGATCATTACAGTGGATACGGGAATCGCCGCCGTCCATGAAGCTGAAGTCGCAAAAGAGCTG
GGCTTAGACGTCATTATTACCGACCACCATGAGCCGGGACCCGAGCTTCCTGACGTATTTGCGATCATCCACCCGAAGCA
GCCGGGCTGCGGCTATCCGTTTAAAGAATTGGCCGGCGTCGGTGTGGCGTTTAAGCTTGCCCATGCGCTTACGGGTTCTT
TGCCGGAAGCTATGCTGGACTTGGCGGCAATCGGTACGGTTGCAGATCTGGTGCCTCTTCATGATGAAAACAGATTAATT
GCGTCACTTGGGATCGAAAGGCTTCGAACGACAAATCGGCCGGGCCTCAAAGCTCTGATTAAGCTTTCCGGCGGAGATAT
CGGACAAGCTGATGAGGAAACGATCGGATTTCAGGTCGCGCCGAGGCTCAATGCCGTCGGAAGAATAGAACAGGCCGATC
CCGCCGTGCATCTGCTGATGACCGAAGATCAGTTTGAAGCGGAGGAACTGGCTTCGGATATTGACCTGCTGAATAAAGAG
CGGCAGAAAATGGTGAAAACCATGACCGAAGAGGCTGTTGAAATGGTTCAGCAGCAAGGCGGAAACCCGTCTGCCATTGT
AGTGGCAAAAGCCGGCTGGAATCCGGGAGTCGTCGGTATTGTCGCATCGAAGCTGGCTGAACGTTTTTACCGGCCCGCTG
TTGTGCTTGGGATAGATGAGGAGCAGGGCATCGCTAAAGGGTCGGCGCGAAGCATCAGAGGCTTCAACCTTTTTACGAGC
CTTTCACAGTGCAGAGACATTCTTCCTCATTTCGGCGGGCATCCGATGGCGGCAGGAATGACGTTGAAGGCTGAAGATGT
GGCGGAATTAAGGGACAGGCTGAATCAAATGGCTGAAGAAACCCTTACGGAAGAGGATTTTATTCCCGTTCAGGAGGTTG
ATCTTGTCTGCGGCGTTGAAGACATAACCGTTGAAAGCATTACAGAATTGAATATGCTGTCGCCGTTCGGCATGCTTAAC
CCTAAACCGCATGTGATGGTGAAAAATGCCGTGCTTGATGATGTGCGCCTGATCGGAGCGAATAAAAATCACGTGAAAAT
GACGGTGAAAAGTGATTCCGCCGTCCTTGACTGCGTCGGCTTTAACAAAGGAGAGCTTGAAGCCGGAGTTGTCCCCGGAT
CAACGATTTCCATTGTCGGCGAGATGTCCATTAATGAATGGAACAACAGAAAGAAACCACAGCTAATGATTAAAGATGCT
GCGGTGCCGGAATGGCAGCTTTTTGATATCAGGGGCAAGCGGACGTGGGAAGACACGGTCACCGCGCTGCCGAAGGAAAA
ACGGGTGCTCATCAGCTTTAACGAAAAAACCGCTCAGAAGCTTCGTGAAAAAGAGCTGTCGGGCGAGCTGGCCGGGATCA
CTTCGGAATCCGAGGCGGAAGCTCTCCAGCTTGACGGAAAATATGCCGTTTTTCTGGATGTGCCGCCGTCAGCGGATCGC
CTGCAGCATTTGCTTGACGGAAAGAAGCCGGAACGGATTTATTTTGTTTTTATCAATCATGAAGAACATTTCCTGTCCTC
ATTTCCATCGCGGAATGATTTCAAGTGGTTTTACGCATTTCTCTTAAAAAGGGGCGCTTTTGACTTCGGCAAGCATGGTT
CAGAGCTTGCGAGACACAGAGGATGGTCAATTGACACAATCAATTTCATGACAAAGGTGTTTTTTGATCTCGGTTTTGTT
AAAATAGAAGATGGTGTGCTGTCTGTCGTGAGCGGCGCGAAAAAACGCGACTTGGCAGATTCGCAAACCTATCAGGTAAA
ACAGCAGCTGATGGAATTAGACCAGAAGCTCAATTATTCCGCAGCCCATGAGCTGAAGGAATGGCTGAATAAGCTGATGA
ACCAAGGTTCAGAAGCTTATGAAAGTACGAGGAGGACATAG

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(453-559)

DHHA1 domain (DHHA1)

(316-437)

DHH family (DHH)

(83-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

82.061

100

0.821