Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   MK616_RS12515 Genome accession   NZ_CP092778
Coordinates   2573644..2574411 (-) Length   255 a.a.
NCBI ID   WP_007408287.1    Uniprot ID   A0AAP3YCF1
Organism   Bacillus amyloliquefaciens strain WF2020     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2568644..2579411
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK616_RS12495 (MK616_12495) - 2568846..2569658 (-) 813 WP_053284929.1 pyruvate, water dikinase regulatory protein -
  MK616_RS12500 (MK616_12500) ccpN 2569684..2570322 (-) 639 WP_003152989.1 transcriptional regulator CcpN -
  MK616_RS12505 (MK616_12505) glyS 2570423..2572462 (-) 2040 WP_029973407.1 glycine--tRNA ligase subunit beta -
  MK616_RS12510 (MK616_12510) glyQ 2572455..2573342 (-) 888 WP_003152984.1 glycine--tRNA ligase subunit alpha -
  MK616_RS12515 (MK616_12515) recO 2573644..2574411 (-) 768 WP_007408287.1 DNA repair protein RecO Machinery gene
  MK616_RS12520 (MK616_12520) - 2574448..2574591 (-) 144 WP_007408286.1 YqzL family protein -
  MK616_RS12525 (MK616_12525) era 2574747..2575652 (-) 906 WP_007408285.1 GTPase Era -
  MK616_RS12530 (MK616_12530) - 2575633..2576043 (-) 411 WP_003152976.1 cytidine deaminase -
  MK616_RS12535 (MK616_12535) - 2576167..2576541 (-) 375 WP_003152974.1 diacylglycerol kinase family protein -
  MK616_RS12540 (MK616_12540) ybeY 2576522..2576995 (-) 474 WP_003152973.1 rRNA maturation RNase YbeY -
  MK616_RS12545 (MK616_12545) - 2576996..2579128 (-) 2133 WP_053284931.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29292.91 Da        Isoelectric Point: 8.5625

>NTDB_id=660211 MK616_RS12515 WP_007408287.1 2573644..2574411(-) (recO) [Bacillus amyloliquefaciens strain WF2020]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPISPQTARLLRLFYYFDIGRLGSVSLKQETKNEIKRVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=660211 MK616_RS12515 WP_007408287.1 2573644..2574411(-) (recO) [Bacillus amyloliquefaciens strain WF2020]
ATGCTGACTAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGACGTGATCACCTTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGGTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATTTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CCAAAAACGAAATTAAACGGGTCATTGATCTGTATTACGAGGAATATTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GATCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

89.02

100

0.89