Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   MKF36_RS11530 Genome accession   NZ_CP092750
Coordinates   2369800..2370600 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain KS04AU     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2364800..2375600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKF36_RS11500 (MKF36_11500) - 2365339..2365971 (+) 633 WP_007408365.1 helix-turn-helix domain-containing protein -
  MKF36_RS11505 (MKF36_11505) - 2366268..2366405 (+) 138 WP_162839613.1 hypothetical protein -
  MKF36_RS11510 (MKF36_11510) - 2366499..2367617 (+) 1119 WP_015240192.1 NADH-dependent flavin oxidoreductase -
  MKF36_RS11515 (MKF36_11515) - 2367744..2367956 (+) 213 WP_007408362.1 spore germination protein -
  MKF36_RS11520 (MKF36_11520) - 2368082..2368294 (+) 213 WP_003153181.1 spore germination protein -
  MKF36_RS11525 (MKF36_11525) alr 2368470..2369651 (+) 1182 WP_007408361.1 alanine racemase -
  MKF36_RS11530 (MKF36_11530) spo0A 2369800..2370600 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  MKF36_RS11535 (MKF36_11535) spoIVB 2370881..2372161 (-) 1281 WP_007408360.1 SpoIVB peptidase -
  MKF36_RS11540 (MKF36_11540) recN 2372325..2374055 (-) 1731 WP_042635350.1 DNA repair protein RecN Machinery gene
  MKF36_RS11545 (MKF36_11545) argR 2374087..2374536 (-) 450 WP_003153173.1 transcriptional regulator ArgR -
  MKF36_RS11550 (MKF36_11550) - 2374633..2375478 (-) 846 WP_007612503.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=660024 MKF36_RS11530 WP_003153177.1 2369800..2370600(-) (spo0A) [Bacillus velezensis strain KS04AU]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=660024 MKF36_RS11530 WP_003153177.1 2369800..2370600(-) (spo0A) [Bacillus velezensis strain KS04AU]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCAGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATTCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCTGCGCAAAGCAGCATTATCCGCAGCCAGCCTGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAGCCAACCAATTCAGAGTTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97