Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MKF36_RS01710 Genome accession   NZ_CP092750
Coordinates   313721..314158 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain KS04AU     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 308721..319158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKF36_RS01685 (MKF36_01685) - 309743..310877 (+) 1135 Protein_327 zinc-dependent alcohol dehydrogenase -
  MKF36_RS01690 (MKF36_01690) - 310892..311326 (+) 435 WP_007409358.1 RDD family protein -
  MKF36_RS01695 (MKF36_01695) - 311398..311721 (+) 324 WP_007409357.1 YckD family protein -
  MKF36_RS01700 (MKF36_01700) - 311825..313261 (+) 1437 WP_042634804.1 family 1 glycosylhydrolase -
  MKF36_RS01705 (MKF36_01705) nin/comJ 313302..313700 (-) 399 WP_020955304.1 competence protein ComJ Regulator
  MKF36_RS01710 (MKF36_01710) nucA/comI 313721..314158 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  MKF36_RS01715 (MKF36_01715) hxlB 314516..315073 (-) 558 WP_007409354.1 6-phospho-3-hexuloisomerase -
  MKF36_RS01720 (MKF36_01720) hxlA 315070..315705 (-) 636 WP_015239130.1 3-hexulose-6-phosphate synthase -
  MKF36_RS01725 (MKF36_01725) - 315937..316299 (+) 363 WP_007409352.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=659993 MKF36_RS01710 WP_003156588.1 313721..314158(-) (nucA/comI) [Bacillus velezensis strain KS04AU]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=659993 MKF36_RS01710 WP_003156588.1 313721..314158(-) (nucA/comI) [Bacillus velezensis strain KS04AU]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641