Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MKF36_RS00585 Genome accession   NZ_CP092750
Coordinates   101827..103206 (+) Length   459 a.a.
NCBI ID   WP_014304212.1    Uniprot ID   -
Organism   Bacillus velezensis strain KS04AU     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 96827..108206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKF36_RS00565 (MKF36_00565) ctsR 97178..97642 (+) 465 WP_003156396.1 transcriptional regulator CtsR -
  MKF36_RS00570 (MKF36_00570) - 97656..98213 (+) 558 WP_007410387.1 UvrB/UvrC motif-containing protein -
  MKF36_RS00575 (MKF36_00575) - 98213..99304 (+) 1092 WP_003156398.1 protein arginine kinase -
  MKF36_RS00580 (MKF36_00580) clpC 99301..101733 (+) 2433 WP_015239036.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  MKF36_RS00585 (MKF36_00585) radA 101827..103206 (+) 1380 WP_014304212.1 DNA repair protein RadA Machinery gene
  MKF36_RS00590 (MKF36_00590) disA 103210..104292 (+) 1083 WP_007410390.1 DNA integrity scanning diadenylate cyclase DisA -
  MKF36_RS00595 (MKF36_00595) - 104408..105508 (+) 1101 WP_003156403.1 PIN/TRAM domain-containing protein -
  MKF36_RS00600 (MKF36_00600) ispD 105521..106219 (+) 699 WP_020955218.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MKF36_RS00605 (MKF36_00605) ispF 106212..106688 (+) 477 WP_003156407.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49538.97 Da        Isoelectric Point: 7.1316

>NTDB_id=659990 MKF36_RS00585 WP_014304212.1 101827..103206(+) (radA) [Bacillus velezensis strain KS04AU]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVQTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQMLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPEANADGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=659990 MKF36_RS00585 WP_014304212.1 101827..103206(+) (radA) [Bacillus velezensis strain KS04AU]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAATGGATGGGAAAGTGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCGGAAGAACCCCGCGTTCAAACAAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTGAAAGGCTCGTTAGTATTAATCGGCGGCGATCCCGGGATCGGAAAATCAACGCT
CCTTCTCCAAGTATCCGCACAATTAGCTGATACGGCCGGCAGCGTGCTTTACATTTCAGGGGAAGAATCCGTGAAGCAAA
CGAAGCTGCGCGCCGACAGACTTGGAATCAACAGCCAAATGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTTTACCAAAGTGATATCACCTCAGC
TCCCGGCAGTGTATCACAAGTAAGAGAGTGTACGGCCGAATTGATGAAAATTGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGGCACGTCACAAAAGAAGGATCGATCGCGGGGCCGAGATTGCTTGAGCACATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCACCATACATTCCGTATTTTGCGGGCCGTAAAAAACCGTTTCGGCTCCACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAGGGGCTTACGGAAGTGCTGAACCCTTCTGAAATTTTCTTGGAAGAGCGTTCTGCCGGGGCATCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGAACGAGACCGATTCTGGTTGAAATCCAGGCGCTCATTTCACCGACCAGTTTT
GGAAACCCGCGCCGCATGGCTACAGGCATTGACCACAACAGGGTATCGTTAATTATGGCTGTGCTTGAGAAGCGGGTGGG
TCTTTTGCTTCAAAATCAAGATGCGTATTTAAAGGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCCTCAAGCTTCAGAGATACACCGCCGAATCCCGCAGATTGCTTTATCGGTGAAGTGGGTCTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAGGAAGCGGCAAAACTCGGCTTCAAGCGCATGATTATACC
TGAGGCGAATGCAGACGGGTGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(71-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

93.45

99.782

0.932
  radA Streptococcus pneumoniae Rx1

63.797

98.693

0.63
  radA Streptococcus pneumoniae D39

63.797

98.693

0.63
  radA Streptococcus pneumoniae R6

63.797

98.693

0.63
  radA Streptococcus pneumoniae TIGR4

63.797

98.693

0.63
  radA Streptococcus mitis NCTC 12261

63.797

98.693

0.63
  radA Streptococcus mitis SK321

63.576

98.693

0.627