Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MJ608_RS18900 Genome accession   NZ_CP092733
Coordinates   4054253..4055638 (-) Length   461 a.a.
NCBI ID   WP_256629456.1    Uniprot ID   -
Organism   Comamonas thiooxydans strain CHJ01     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4049253..4060638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ608_RS18875 (MJ608_18845) - 4050332..4051096 (+) 765 WP_256629447.1 glycosyltransferase family 25 protein -
  MJ608_RS18880 (MJ608_18850) - 4051086..4052165 (-) 1080 WP_256629449.1 glycosyltransferase -
  MJ608_RS18885 (MJ608_18855) - 4052246..4052455 (-) 210 WP_003059801.1 zinc-finger domain-containing protein -
  MJ608_RS18890 (MJ608_18860) - 4052600..4053538 (-) 939 WP_256629452.1 branched-chain amino acid transaminase -
  MJ608_RS18895 (MJ608_18865) - 4053620..4054072 (-) 453 WP_256629454.1 glycerate kinase -
  MJ608_RS18900 (MJ608_18870) radA 4054253..4055638 (-) 1386 WP_256629456.1 DNA repair protein RadA Machinery gene
  MJ608_RS18910 (MJ608_18880) - 4056061..4057368 (-) 1308 WP_256629457.1 Glu/Leu/Phe/Val dehydrogenase -
  MJ608_RS18915 (MJ608_18885) - 4057578..4058501 (+) 924 WP_256629459.1 fumarylacetoacetate hydrolase family protein -
  MJ608_RS18920 (MJ608_18890) - 4058656..4059765 (-) 1110 WP_256629461.1 PPK2 family polyphosphate kinase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 48059.37 Da        Isoelectric Point: 7.6670

>NTDB_id=659916 MJ608_RS18900 WP_256629456.1 4054253..4055638(-) (radA) [Comamonas thiooxydans strain CHJ01]
MAKEKTTFTCNACGGTSPRWLGKCPGCGAWNTLVETVADSASGGKNRLSQPQGYAGLANAQPVTPLSAIEAQDVARTPSG
IEELDRVLGGGVVEGGVVLIGGDPGIGKSTLLLQAMDALHRIGLPTLYVTGEESGAQVALRSRRLGLDNSQVNVLAEIQL
EKILATVEATQPAVVVIDSIQTVYSDQLSSAPGSVAQVRECAAHLTRAAKTTGITIILVGHVTKDGALAGPRVLEHMVDT
VLYFEGDTHSNFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVTNPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQALVD
QGGPAARRLSVGLDRDRLAMLLAVLNRHAGVACADQDVFVNAVGGVRINEPAADLAVMLAITSSLRGKSLPKGFIAFGEV
GLAGEVRPAPRGQERLKEAAKLGFTVAVVPKANAPKKPIAGLTIHAVERVDEAINIVRGIG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=659916 MJ608_RS18900 WP_256629456.1 4054253..4055638(-) (radA) [Comamonas thiooxydans strain CHJ01]
ATGGCCAAAGAGAAAACCACTTTCACCTGCAACGCCTGTGGCGGCACCAGCCCGCGCTGGCTGGGCAAATGCCCGGGCTG
CGGCGCCTGGAACACACTGGTCGAGACCGTGGCCGACTCCGCCTCGGGCGGCAAGAACCGCCTGAGCCAGCCTCAAGGCT
ATGCAGGTCTGGCCAATGCCCAGCCCGTCACCCCGCTGTCGGCCATCGAGGCCCAGGATGTGGCGCGCACGCCCAGCGGC
ATCGAGGAGCTGGACCGCGTGCTGGGCGGCGGCGTGGTCGAAGGCGGCGTGGTGTTGATTGGCGGAGACCCAGGAATAGG
TAAGTCCACACTTCTTTTGCAGGCCATGGATGCCTTGCATCGTATTGGCCTGCCCACGCTCTATGTGACGGGCGAGGAAA
GCGGCGCCCAGGTGGCACTGCGCTCGCGCCGGCTGGGGCTGGACAACAGCCAGGTCAATGTGCTGGCCGAGATCCAGCTC
GAGAAAATCCTGGCCACCGTGGAGGCCACTCAGCCCGCCGTGGTGGTGATTGACTCGATCCAGACGGTGTATTCGGACCA
GCTCTCCTCGGCTCCAGGATCCGTGGCCCAAGTACGCGAATGCGCGGCCCATCTGACGCGCGCGGCCAAGACCACGGGCA
TCACCATCATTTTGGTGGGCCATGTGACCAAGGACGGCGCGCTGGCCGGCCCGCGCGTGCTCGAGCACATGGTGGACACG
GTGCTCTACTTCGAAGGCGACACGCACAGCAACTTCCGCCTGGTGCGCGCGATCAAGAACCGCTTTGGCGCCGTCAACGA
GATCGGCGTCTTCGCGATGACGGAAAAAGGCCTCAAGGGCGTGACCAACCCCAGCGCCATCTTTCTGAGTCAGCACAGCG
AGCCCGTGCCCGGCAGTTGCGTGCTGGTGACGCTGGAGGGCACGCGCCCCATGCTGGTGGAAATCCAGGCCCTTGTGGAC
CAGGGCGGCCCGGCCGCGCGCCGCCTCTCGGTCGGCCTGGACCGCGACCGGCTGGCCATGCTGCTGGCGGTGCTCAACCG
CCATGCGGGCGTGGCCTGCGCCGACCAGGATGTCTTCGTCAACGCCGTGGGCGGTGTGCGCATCAATGAGCCTGCGGCTG
ACCTGGCGGTGATGTTGGCAATCACTTCCAGCCTGCGCGGCAAGTCGCTGCCCAAGGGCTTTATTGCGTTTGGCGAGGTC
GGTCTGGCCGGTGAGGTGCGCCCAGCACCTCGCGGCCAGGAGCGACTCAAGGAGGCGGCAAAGCTGGGATTCACCGTCGC
AGTGGTCCCCAAGGCCAATGCCCCCAAGAAGCCCATCGCCGGCCTGACCATTCATGCCGTGGAGCGCGTGGATGAAGCCA
TCAACATCGTGCGCGGCATTGGTTGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-226)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(364-455)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.02

99.566

0.488
  radA Streptococcus mitis NCTC 12261

47.598

99.349

0.473
  radA Streptococcus pneumoniae D39

48.438

97.18

0.471
  radA Streptococcus pneumoniae TIGR4

48.438

97.18

0.471
  radA Streptococcus pneumoniae R6

48.438

97.18

0.471
  radA Streptococcus pneumoniae Rx1

48.438

97.18

0.471
  radA Streptococcus mitis SK321

49.425

94.36

0.466