Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MJ608_RS16940 Genome accession   NZ_CP092733
Coordinates   3619602..3621203 (-) Length   533 a.a.
NCBI ID   WP_256629047.1    Uniprot ID   -
Organism   Comamonas thiooxydans strain CHJ01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3614602..3626203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ608_RS16915 (MJ608_16885) - 3614762..3615253 (-) 492 WP_256629041.1 putative toxin-antitoxin system toxin component, PIN family -
  MJ608_RS16920 (MJ608_16890) - 3615250..3616548 (-) 1299 WP_003068206.1 class I SAM-dependent RNA methyltransferase -
  MJ608_RS16925 (MJ608_16895) - 3616728..3617066 (+) 339 WP_039050220.1 tRNA-binding protein -
  MJ608_RS16930 (MJ608_16900) - 3617124..3618356 (+) 1233 WP_256629044.1 DUF711 family protein -
  MJ608_RS16935 (MJ608_16905) - 3618492..3619412 (+) 921 WP_080746150.1 aspartyl/asparaginyl beta-hydroxylase domain-containing protein -
  MJ608_RS16940 (MJ608_16910) rpoN/rpoN1 3619602..3621203 (-) 1602 WP_256629047.1 RNA polymerase factor sigma-54 Machinery gene
  MJ608_RS16945 (MJ608_16915) lptB 3621349..3622131 (-) 783 WP_012840179.1 LPS export ABC transporter ATP-binding protein -
  MJ608_RS16950 (MJ608_16920) lptA 3622128..3622784 (-) 657 WP_003068194.1 lipopolysaccharide transport periplasmic protein LptA -
  MJ608_RS16955 (MJ608_16925) - 3622915..3623565 (-) 651 WP_256629049.1 thiol:disulfide interchange protein DsbA/DsbL -
  MJ608_RS16960 (MJ608_16930) - 3623726..3624427 (-) 702 WP_039050223.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 533 a.a.        Molecular weight: 59122.37 Da        Isoelectric Point: 4.6610

>NTDB_id=659912 MJ608_RS16940 WP_256629047.1 3619602..3621203(-) (rpoN/rpoN1) [Comamonas thiooxydans strain CHJ01]
MKPGLSLRVSQHLSLTPQLQQSIQLLQLSTLELAQEVEQMLVDNPFLERAGDEAETAGDAPVDVAASASEYSTSDGIHAE
RDASQAQQATETVAETSNPETPETLNWEADGMDGQDGEWGGESSSEWDTPSASSSTRNRDGDDETDAIDLAREHQSLTDF
LHRQALSLHLGAADMAALRFLIESLSDDGYLEDSLQELAQSLAGDDLEEREELEQHFAIALKLLQSLEPVGVGARDLAEC
LQLQLRDLIRTAEDEGTTEAHMSRLLLAQAICAQPMDLLARRDLRKLTSLCGAREEQLREAMALIARLEPRPGRRFVDVE
RHIVVPDVIVRPVRSGSGRVDFSVQLNPEVMPRLRVHDIYASVLRRHKNSDGHAALQQQLQEARWFIKNIQQRFDTILRV
SEAIVERQKNFFVHGELAMRPLVLRDIADTLGLHESTISRVTTAKYMATPQGTFELKYFFGSGLGTETGGSASSTAVRAL
IKQFVDAEDARKPLSDAKLADMLKEQGIECARRTVAKYREQLKIPTTTLRKAL

Nucleotide


Download         Length: 1602 bp        

>NTDB_id=659912 MJ608_RS16940 WP_256629047.1 3619602..3621203(-) (rpoN/rpoN1) [Comamonas thiooxydans strain CHJ01]
ATGAAGCCAGGTCTCTCGCTACGCGTTTCGCAGCATTTATCGCTTACGCCGCAGCTGCAGCAATCCATTCAGCTGCTGCA
GCTCTCCACCCTGGAGCTGGCGCAGGAAGTCGAGCAGATGCTGGTCGACAACCCTTTTCTGGAACGTGCGGGCGATGAGG
CAGAAACTGCGGGGGATGCTCCTGTAGATGTAGCTGCTAGCGCCAGTGAATACAGCACTTCAGATGGTATTCATGCTGAA
AGAGATGCATCGCAAGCGCAACAAGCTACTGAAACGGTAGCGGAGACATCAAACCCCGAAACCCCGGAAACGCTGAACTG
GGAGGCCGACGGCATGGACGGCCAGGACGGGGAATGGGGCGGCGAATCCAGCAGCGAGTGGGATACGCCCTCGGCCAGCA
GCTCCACGCGCAACCGCGACGGCGATGACGAAACCGATGCCATCGATCTGGCGCGCGAGCACCAGAGCCTGACCGACTTT
CTGCACCGTCAGGCGCTGAGCCTGCATCTTGGTGCGGCGGACATGGCTGCACTGCGCTTTCTCATCGAGTCGCTCAGCGA
CGACGGCTATCTCGAGGATTCCCTGCAGGAGTTGGCGCAGAGCCTGGCTGGCGACGACTTGGAGGAGCGCGAGGAGCTGG
AGCAGCACTTTGCCATTGCGCTGAAGCTGCTGCAAAGCCTGGAGCCCGTGGGTGTGGGCGCGCGCGATCTGGCCGAGTGC
CTGCAGCTGCAGCTGCGCGATCTGATCCGCACGGCCGAGGACGAGGGCACGACCGAGGCGCATATGAGCCGGCTGCTGCT
GGCGCAGGCCATCTGTGCCCAGCCCATGGACCTGCTGGCGCGGCGCGATCTGCGCAAGCTCACCAGCCTGTGCGGTGCGC
GAGAGGAGCAACTGCGCGAAGCCATGGCCCTGATCGCCAGGCTGGAGCCGCGTCCGGGGCGCCGCTTTGTCGACGTGGAA
CGCCATATCGTGGTGCCCGATGTCATCGTGCGCCCGGTGCGGTCCGGCTCGGGCCGGGTGGACTTCTCCGTGCAGCTCAA
CCCCGAGGTCATGCCGCGTCTGCGCGTGCACGACATCTATGCCAGCGTGCTGCGCCGCCACAAGAACAGCGACGGCCATG
CGGCGCTGCAGCAGCAACTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCTTGCGTGTT
TCCGAAGCCATCGTGGAGCGGCAGAAGAACTTCTTTGTGCATGGCGAGCTGGCCATGCGCCCGCTGGTGCTGCGCGATAT
CGCCGACACCCTGGGCCTGCACGAGTCCACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCACGCCCCAGGGTACCT
TCGAGCTCAAGTATTTCTTCGGTTCGGGCCTGGGCACGGAAACCGGCGGCAGTGCTTCCAGCACGGCCGTGCGTGCGCTG
ATCAAGCAGTTTGTCGATGCCGAAGATGCCAGAAAGCCGCTGTCGGACGCCAAGCTGGCCGATATGCTCAAGGAGCAGGG
CATCGAGTGCGCGCGCCGCACCGTCGCCAAATACCGCGAGCAGCTCAAGATACCGACGACCACGCTGCGCAAGGCGCTGT
AG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-360)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(375-531)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.746

100

0.51