Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   MKW31_RS05505 Genome accession   NZ_CP092715
Coordinates   1103516..1107205 (+) Length   1229 a.a.
NCBI ID   WP_349403733.1    Uniprot ID   -
Organism   Bacillus velezensis strain 37-1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1098516..1112205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKW31_RS05490 (MKW31_05480) - 1098597..1099106 (-) 510 WP_007409173.1 ferritin-like domain-containing protein -
  MKW31_RS05495 (MKW31_05485) - 1099175..1099888 (-) 714 WP_079891285.1 DUF421 domain-containing protein -
  MKW31_RS05500 (MKW31_05490) addB 1100029..1103529 (+) 3501 WP_244805638.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MKW31_RS05505 (MKW31_05495) addA 1103516..1107205 (+) 3690 WP_349403733.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MKW31_RS05510 (MKW31_05500) sbcD 1107270..1108442 (+) 1173 WP_003155146.1 exonuclease subunit SbcD -
  MKW31_RS05515 (MKW31_05505) sbcC 1108439..1111831 (+) 3393 WP_094032925.1 exonuclease subunit SbcC -
  MKW31_RS05520 (MKW31_05510) - 1111847..1112140 (+) 294 WP_007409167.1 hypothetical protein -

Sequence

Protein


Download         Length: 1229 a.a.        Molecular weight: 140645.81 Da        Isoelectric Point: 5.1529

>NTDB_id=659824 MKW31_RS05505 WP_349403733.1 1103516..1107205(+) (addA) [Bacillus velezensis strain 37-1]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITAEEDPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FFELADRYTTDRHDLDLQDLVKRVYEYSRSHPDPEAWLQSFVRLYDVTEESKMEELPFYQYVREDAEMALFGAKQKLEKA
LELTKAPGGPASRADNFLDDLQQIEELISCRHDFDALYERVPAVSFKRAKAVKGDEFDKATDLRNGAKKLIEKVKTDYFT
RSPQDHLKSLTDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAVDEEGRRVPSEAAVYYQDQFHEVL
VDEYQDTNLVQESILQLVKSGNEETGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTESGEGAGQKIDLNQNFRSRSDIL
DSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNDAAKTELLLIDSADGADSSEDAEDFETVHWEAKAIAGEIRKLVSS
PFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDIPLASV
LRSPIVGCDENELALIRLEKKKAPFYEALKAYIANADRHDDLYQKLRTFYDSLQKWRSFSTNHSVSELIWEVYRDTGYFD
YAGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARTLSEQEDVVRLMTIHSSKGLEFPVVF
TAGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFLVGSCK
NREKQLAKWQAQADRADWLLSEFDRYQASSYLDFIGPALIRHRDMEAHRTPGLSSSEDIARDPSRFHIRMLQQSELLEEN
PKERAEEKSTRLKAIQQGEPIPDSFSFDDQARRLLEWEYPYRELTAIRTKQSVSELKRKQEYEDEYSGRSLIKPSGDTLL
YRRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTAEEAERTVRMLYEKELLTEEQQEAIDIEEIVQFFGTEIGKDLLGA
LRIDREVPFSMALPAGEVYKDAETAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYETQIEL
YTKAVEQITKTKVKGRALYFFDGGHVLTL

Nucleotide


Download         Length: 3690 bp        

>NTDB_id=659824 MKW31_RS05505 WP_349403733.1 1103516..1107205(+) (addA) [Bacillus velezensis strain 37-1]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCTGCCGCAGCCGGATCAGGTAAAACGGCCGTGCTCGTGGAACGGATGATCAGAAAGATAACCGCGGAGGAAGATC
CGGTTGATGTTGACCGGCTCCTTGTGGTGACCTTTACAAACGCCTCAGCAGCGGAGATGAAGCATCGGATCGCGGAAGCA
TTAGAAAAAGAGCTCGCCAAAAATCCGGGATCTCTGCATATCAGACGGCAGCTCTCTCTATTAAATCGGGCGAGCATTTC
GACCCTTCACTCTTTCTGTCTGCAAGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCCGGTTTTCGGATGGCTG
ACCAGACGGAAGGCGAATTGCTCGGAGACGAAGTGCTTGATGAGCTGTTTGAAGATGAATACGCAAAAGGGAATCAGGCG
TTTTTTGAGCTTGCAGACAGATACACGACAGACAGGCATGATCTGGATCTTCAAGACCTTGTCAAACGGGTGTACGAGTA
TTCCCGGTCGCATCCTGACCCGGAAGCGTGGCTGCAAAGCTTTGTCCGCCTGTATGACGTGACGGAAGAAAGCAAAATGG
AAGAGCTTCCGTTTTACCAATATGTGAGAGAAGATGCTGAAATGGCGCTCTTCGGGGCAAAACAAAAGCTTGAGAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCCTCGCGCGCTGATAATTTTCTGGATGACCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTCGATGCGCTGTATGAACGGGTTCCCGCAGTCTCCTTTAAGCGGGCGAAGGCGGTAAAGG
GCGATGAATTTGACAAAGCGACCGATTTGCGAAACGGCGCGAAGAAACTGATCGAAAAGGTAAAGACCGATTACTTTACA
AGAAGTCCGCAAGATCACTTAAAGAGTTTAACAGATATGAAGCCTGTCATCGAAACGCTCGTTCAGCTCGTGATATCGTA
CGGAAAACGATTTGAAGCGGCGAAACAAGAAAAATCAATTATTGATTTCTCAGATTTGGAACATTACTGCCTGGCGATTT
TAACTGCGGTTGATGAAGAAGGCCGGCGAGTGCCGAGCGAAGCGGCCGTTTATTATCAAGACCAGTTCCATGAAGTTCTG
GTTGATGAATATCAAGATACGAATCTTGTGCAAGAGTCTATCCTGCAGCTTGTTAAAAGCGGGAATGAAGAGACGGGAAA
TCTGTTTATGGTAGGCGACGTTAAACAATCGATCTACCGTTTCAGACTTGCTGAGCCGCTGCTGTTTCTCGGGAAATATA
AAAGGTTTACAGAAAGCGGGGAAGGCGCGGGGCAGAAAATTGACCTGAACCAAAATTTCCGCAGCCGCTCCGATATTTTG
GACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGAAAAATCGGCGAAGTTGATTACGATGAACAGGCGGCATTGAA
GCTCGGCGCATCGTATCCGCCGAATGACGCGGCAAAAACGGAACTGCTGCTCATTGACAGCGCTGATGGCGCAGACAGCT
CCGAGGATGCCGAAGACTTTGAAACTGTGCATTGGGAAGCGAAAGCGATAGCCGGGGAGATCCGAAAGCTTGTCTCATCT
CCGTTTAAGGTATATGACGGCAAAACAAAAACACACCGGAATATCCAGTACCGGGATATCGTAATTTTGCTGCGCTCCAT
GCCGTGGGCGCCGCAATTAATGGAAGAGCTGAAAAACCAGGGCATTCCGGTGTACGCCAATTTGACGTCGGGTTACTTTG
AAGCCGTGGAGGTAGCTGCGGCGCTTTCAGTCTTGAAGGTGATTGATAATCCGTATCAGGATATTCCGCTTGCTTCCGTG
CTGCGCTCGCCCATCGTCGGCTGTGACGAAAATGAACTTGCGCTGATACGCCTTGAAAAGAAAAAAGCGCCGTTTTATGA
AGCGCTGAAAGCATATATAGCGAACGCTGACAGGCATGATGACCTGTATCAAAAACTGAGAACCTTTTATGACAGTCTGC
AAAAGTGGCGGTCATTTTCGACGAATCATTCGGTGTCTGAGCTGATTTGGGAAGTATACCGCGATACCGGATATTTTGAC
TATGCAGGCGGCATGCCTGGCGGAAAGCAGCGCCAGGCCAATCTGCGCGTCTTGTATGACCGGGCGCGCTCGTACGAAGC
GACGGCGTTCCGCGGGTTATTCCGCTTTTTGCGGTTTATTGAACGGATGCAGGAGCGCGGCGATGATCTGGGGACGGCCC
GGACGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATCCACAGCAGCAAAGGACTTGAATTCCCCGTCGTTTTC
ACCGCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATACCTGCTTGATAAAGAGCTCGGTTTCGGCAC
GAAGTATATTCATCCGGAACTCAGAATCAGCTATCCGACTCTTCCGCTTGTGGCGATGAAGAAAAAGATGCGCAGAGAGC
TCTTATCCGAAGAATTGCGCGTATTGTATGTGGCGCTGACCAGAGCGAAGGAAAAGCTGTTTCTCGTCGGTTCCTGCAAA
AACCGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCAGACCGGGCCGATTGGCTTCTTTCTGAATTCGACCGCTATCA
GGCGTCATCTTATTTGGACTTTATCGGGCCGGCGCTCATCCGCCACCGTGACATGGAGGCGCACAGGACGCCGGGACTTT
CCTCATCTGAGGATATCGCGCGCGACCCGTCGCGTTTTCACATCCGGATGCTGCAGCAAAGTGAATTGCTGGAAGAGAAT
CCGAAAGAACGTGCTGAAGAAAAGAGCACACGTCTCAAAGCGATTCAGCAGGGCGAGCCGATTCCTGATTCGTTTTCATT
TGATGATCAGGCGCGCCGTCTGCTTGAATGGGAATATCCGTACCGAGAGCTGACGGCTATCAGAACGAAGCAGTCAGTTT
CTGAGCTGAAGAGAAAGCAAGAATACGAGGATGAATACAGCGGCCGCTCTCTCATCAAACCGTCAGGAGACACGCTTTTA
TACAGGCGTCCCGGTTTTATGATGAAAAAAGGGCTGACAGCCGCCGAAAAAGGGACGGCGATGCATACGGTGATGCAGCA
CATACCGCTGACGCACGTTCCGACTGCCGAAGAAGCGGAGCGGACGGTCCGGATGCTTTACGAAAAAGAGCTGCTGACAG
AAGAACAGCAGGAAGCTATTGATATAGAAGAAATTGTCCAGTTTTTCGGAACTGAAATCGGGAAAGACCTTCTCGGGGCG
CTGCGTATTGACCGGGAAGTGCCGTTCAGTATGGCGCTTCCGGCCGGCGAGGTGTACAAGGATGCCGAAACGGCCGGTGA
ACCGCTGCTCGTGCAGGGGATTATTGACTGCCTCTACGAAACTGCGGACGGTCTCTATTTGCTGGACTATAAAACAGATC
GGATAGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCTCCGATTCTGCAAAAAAGGTATGAAACACAAATCGAATTG
TATACAAAAGCGGTTGAACAAATTACAAAAACGAAAGTGAAAGGGCGTGCGCTCTATTTCTTCGATGGAGGTCACGTTCT
TACACTGTAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-456)

UvrD-like helicase C-terminal domain (UvrD_C)

(467-876)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1050-1221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

81.929

100

0.823